Results 41 to 50 of about 37,268 (281)

Initial evaluation of a mobile SARS-CoV-2 RT-LAMP testing strategy [PDF]

Journal of Biomolecular Techniques : JBT, 2020
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) control in the United States remains hampered, in part, by testing limitations. We evaluated a simple, outdoor, mobile, colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay workflow where self-collected saliva is tested for SARS-CoV-2 RNA.
Newman, Christina M., Ramuta, Mitchell D., McLaughlin, Matthew T., Wiseman, Roger W., Karl, Julie A., Dudley, Dawn M., Stauss, Miranda R., Maddox, Robert J., Weiler, Andrea M., Bliss, Mason I., Fauser, Katrina N., Haddock III, Luis A., Shortreed, Cecilia G., Haj, Amelia K., Accola, Molly A., Heffron, Anna S., Bussan, Hailey E., Reynolds, Matthew R., Harwood, Olivia E., Moriarty, Ryan V., Stewart, Laurel M., Crooks, Chelsea M., Prall, Trent M., Neumann, Emma K., Somsen, Elizabeth D., Burmeister, Corrie B., Hall, Kristi L., Rehrauer, William M., Friedrich, Thomas C., O’Connor, Shelby L., O’Connor, David H.   +30 more
openaire   +4 more sources

Comparative Diagnostic Performance of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Kit for the Rapid Detection of SARS-CoV-2

Pathogens, 2021
Although the reverse transcription-polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described.
Alexander Domnich, Andrea Orsi, Donatella Panatto, Vanessa De Pace, Valentina Ricucci, Patrizia Caligiuri, Giulia Guarona, Valerio Chessa, Diego Ferone, Simona Boccotti, Bianca Bruzzone, Giancarlo Icardi   +11 more
doaj   +1 more source

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

The Plant Pathology Journal, 2015
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its
Joojin Jeong, Sang-Yun Cho, Wang-Hyu Lee, Kui-jae Lee, Ho-Jong Ju   +4 more
doaj   +1 more source

Evaluation of a novel direct RT-LAMP assay for the detection of SARS-CoV-2 from saliva samples in asymptomatic individuals

Journal of Clinical Virology Plus, 2022
Large scale screening of health care workers and the general population for asymptomatic COVID-19 infection requires modalities that are amenable to testing at scale while retaining acceptable levels of sensitivity and specificity.This study evaluated a ...
Benjamin Brown, Robert William O'Hara, Malcolm Guiver, Emma Davies, Andrew Birtles, Hamzah Farooq, Arpana Verma, Hui Guo, Katharine Hayden, Nicholas Machin   +9 more
doaj   +1 more source

Detektion des Kartoffelspindelknollen Viroids mit Hilfe der Loop Mediated Isothermal Amplification [PDF]

, 2011
PSTV ist ein hoch infektiöses Viroid, das in Kartoffeln verkleinerte und spindelähnliche Knollen verursacht. Um Ernteverlusten vorzubeugen, ist eine Detektion in frühen Infektionsstadien von großer Bedeutung.
Frohme, Marcus, Glökler, Jörn, Kadolsky, Marianne, Schulze, Katja   +3 more
core   +1 more source

A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture-reverse transcriptase loop-mediated isothermal amplification assay.

PLoS ONE, 2019
Severe strains of Citrus tristeza virus (CTV) cause quick decline and stem pitting resulting in significant economic losses in citrus production. A immunocapture reverse-transcriptase loop-mediated amplification (IC-RT-LAMP) assay was developed in this ...
Vijayanandraj Selvaraj, Yogita Maheshwari, Subhas Hajeri, Raymond Yokomi   +3 more
doaj   +1 more source

Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats [PDF]

PeerJ, 2019
Background Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV ...
Xin Sui, Xu Zhang, Dongliang Fei, Zhen Zhang, Mingxiao Ma   +4 more
doaj   +2 more sources

Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP [PDF]

, 2015
In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions.
Ismagilov, Rustem F., Karymov, Mikhail A., Khorosheva, Eugenia M., Selck, David A.   +3 more
core   +2 more sources

Detection of Acute HIV-1 Infection by RT-LAMP

PLOS ONE, 2015
A rapid, cost-effective diagnostic test for the detection of acute HIV-1 infection is highly desired. Isothermal amplification techniques, such as reverse-transcription loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for the development of a rapid nucleic acid amplification test (NAAT) because they are quick ...
Donna L Rudolph, Vickie Sullivan, S Michele Owen, Kelly A Curtis   +3 more
openaire   +4 more sources

Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Scientific Reports, 2023
The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, a high false-positive rate due to misamplification is one of the major limitations.
Galyah Alhamid, Huseyin Tombuloglu, Ebtesam Al-Suhaimi   +2 more
doaj   +1 more source