Results 41 to 50 of about 37,763 (207)

Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay [PDF]

, 2011
Background Rapid identification and differentiation of mosquito-transmitted flaviviruses in acute-phase sera of patients and field-caught vector mosquitoes are important for the prediction and prevention of large-scale epidemics.
Shuhua Li, Meiyu Fang, Bin Zhou, Hongxia Ni, Qiuxia Shen, Hongwei Zhang, Yifang Han, Jianhua Yin, Wenjun Chang, Guozhang Xu, Guangwen Cao   +10 more
core   +1 more source

Initial evaluation of a mobile SARS-CoV-2 RT-LAMP testing strategy [PDF]

Journal of Biomolecular Techniques : JBT, 2020
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) control in the United States remains hampered, in part, by testing limitations. We evaluated a simple, outdoor, mobile, colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay workflow where self-collected saliva is tested for SARS-CoV-2 RNA.
Newman, Christina M., Ramuta, Mitchell D., McLaughlin, Matthew T., Wiseman, Roger W., Karl, Julie A., Dudley, Dawn M., Stauss, Miranda R., Maddox, Robert J., Weiler, Andrea M., Bliss, Mason I., Fauser, Katrina N., Haddock III, Luis A., Shortreed, Cecilia G., Haj, Amelia K., Accola, Molly A., Heffron, Anna S., Bussan, Hailey E., Reynolds, Matthew R., Harwood, Olivia E., Moriarty, Ryan V., Stewart, Laurel M., Crooks, Chelsea M., Prall, Trent M., Neumann, Emma K., Somsen, Elizabeth D., Burmeister, Corrie B., Hall, Kristi L., Rehrauer, William M., Friedrich, Thomas C., O’Connor, Shelby L., O’Connor, David H.   +30 more
openaire   +4 more sources

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane [PDF]

, 2019
In this work, we report digital loop-mediated isothermal amplification (LAMP) or reverse-transcription LAMP (RT-LAMP) on a commercial membrane, without the need for complex chip fabrication or use of specialized equipment.
Hoffmann, Michael R., Huang, Xiao, Lin, Xingyu, Urmann, Katharina, Xie, Xing   +4 more
core   +4 more sources

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

The Plant Pathology Journal, 2015
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its
Joojin Jeong, Sang-Yun Cho, Wang-Hyu Lee, Kui-jae Lee, Ho-Jong Ju   +4 more
doaj   +1 more source

Evaluation of a novel direct RT-LAMP assay for the detection of SARS-CoV-2 from saliva samples in asymptomatic individuals

Journal of Clinical Virology Plus, 2022
Large scale screening of health care workers and the general population for asymptomatic COVID-19 infection requires modalities that are amenable to testing at scale while retaining acceptable levels of sensitivity and specificity.This study evaluated a ...
Benjamin Brown, Robert William O'Hara, Malcolm Guiver, Emma Davies, Andrew Birtles, Hamzah Farooq, Arpana Verma, Hui Guo, Katharine Hayden, Nicholas Machin   +9 more
doaj   +1 more source

A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture-reverse transcriptase loop-mediated isothermal amplification assay.

PLoS ONE, 2019
Severe strains of Citrus tristeza virus (CTV) cause quick decline and stem pitting resulting in significant economic losses in citrus production. A immunocapture reverse-transcriptase loop-mediated amplification (IC-RT-LAMP) assay was developed in this ...
Vijayanandraj Selvaraj, Yogita Maheshwari, Subhas Hajeri, Raymond Yokomi   +3 more
doaj   +1 more source

Detektion des Kartoffelspindelknollen Viroids mit Hilfe der Loop Mediated Isothermal Amplification [PDF]

, 2011
PSTV ist ein hoch infektiöses Viroid, das in Kartoffeln verkleinerte und spindelähnliche Knollen verursacht. Um Ernteverlusten vorzubeugen, ist eine Detektion in frühen Infektionsstadien von großer Bedeutung.
Frohme, Marcus, Glökler, Jörn, Kadolsky, Marianne, Schulze, Katja   +3 more
core   +1 more source

Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification [PDF]

, 2011
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers
Hao-tai Chen, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu   +3 more
core   +1 more source

Multiplex Detection of Aspergillus fumigatus Mycoviruses. [PDF]

, 2018
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Mycoviruses are ...
Latge, Robert Coutts, Selin Özkan-Kotiloğlu   +2 more
core   +6 more sources

Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats [PDF]

PeerJ, 2019
Background Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV ...
Xin Sui, Xu Zhang, Dongliang Fei, Zhen Zhang, Mingxiao Ma   +4 more
doaj   +2 more sources