Results 71 to 80 of about 37,763 (207)

Molecular methods for diagnosing novel coronavirus infection: comparison of loop-mediated isothermal amplification and polymerase chain reaction

Вопросы вирусологии, 2022
Introduction. Currently, the basis for molecular diagnostics of most infections is the use of reverse transcription polymerase chain reaction (RT-PCR). Technologies based on reverse transcription isothermal loop amplification (RT-LAMP) can be used as an ...
V. G. Akimkin, V. V. Petrov, K. V. Krasovitov, N. I. Borisova, I. A. Kotov, E. N. Rodionova, A. S. Cherkashina, L. Yu. Kondrasheva, E. V. Tivanova, K. F. Khafizov   +9 more
doaj   +1 more source

Rapid and Reliable Detection of SARS-CoV-2 Using Direct RT-LAMP

Diagnostics, 2022
Background: The global pandemic coronavirus SARS-CoV-2 has a healthcare, social and economic burden. To limit the spread of the virus, the World Health Organization (WHO) urgently called for extensive screening of suspected individuals; thus, a quick, simple, and sensitive diagnostic assay is always in need.
Sherif A. El-Kafrawy, Mai M. El-Daly, Ahmed M. Hassan, Steve M. Harakeh, Thamir A. Alandijany, Esam I. Azhar   +5 more
openaire   +3 more sources

A Naked-Eye Visual Reverse Transcription Loop-Mediated Isothermal Amplification with Sharp Color Changes for Potential Pen-Side Test of Foot-and-Mouth Disease Virus

Viruses, 2022
Visual loop-mediated isothermal amplification (LAMP) is qualified to be applied in the field to detect pathogens due to its simplicity, rapidity and cost saving. However, the color changes in currently reported visual reverse transcription LAMP (RT-LAMP)
Jie Zhang, Qian Hou, Weimin Ma, Danian Chen, Weibing Zhang, Ashenafi Kiros Wubshet, Yaozhong Ding, Miaomiao Li, Qian Li, Jiao Chen, Junfei Dai, Guohua Wu, Ziteng Zhang, Alexei D. Zaberezhny, Zygmunt Pejsak, Kazimierz Tarasiuk, Muhammad Umar Zafar Khan, Yang Wang, Jijun He, Yongsheng Liu   +19 more
doaj   +1 more source

An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP

Journal of Infection in Developing Countries
Introduction: In the fight against virus-caused pandemics like COVID-19, diagnostic tests based on RT-qPCR are essential, but they are sometimes limited by their dependence on expensive, specialized equipment and skilled personnel.
Diem Hong Tran, Hau Thi Tran, Trang Nguyen Minh Pham, Le Minh Bui, Huong Thi Thu Phung   +4 more
doaj   +1 more source

A reverse transcription loop-mediated isothermal amplification for broad coverage detection of Asian and African Zika virus lineages

BMC Infectious Diseases, 2020
Background Early detection of Zika virus (ZIKV) infection during the viremia and viruria facilitates proper patient management and mosquito control measurement to prevent disease spread.
Boon-Teong Teoh, Kim-Ling Chin, Nur-Izyan Samsudin, Shih-Keng Loong, Sing-Sin Sam, Kim-Kee Tan, Chee-Sieng Khor, Juraina Abd-Jamil, Nurhafiza Zainal, Annelies Wilder-Smith, Keivan Zandi, Sazaly AbuBakar   +11 more
doaj   +1 more source

Artificial Synthesis of Conserved Segment S Gene Fragment of Rift Valley Fever Virus and Preliminary Study of Its Reverse Transcription Loop-Mediated Isothermal Amplification Detection Method [PDF]

, 2016
Purpose: To develop a rapid detection method for Rift Valley fever virus (RVFV) diagnosis.Methods: According to the reference sequences of RVFV published in GenBank, nine overlapping polymerase chain reaction (PCR) primers and four specific reverse ...
Hou, Yihong, Li, Guili, Lin, Xingyu, Peng, Shanzhen, Ren, Ranyang, Wang, Yin, Ya, Houxun, Yang, Zexiao, Yao, Xueping   +8 more
core   +2 more sources

Extraction-Free Colorimetric RT-LAMP Detection of SARS-CoV-2 in Saliva

Diagnostics, 2023
The pandemic situation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need of fast, simple, and cost-effective tests for the diagnosis of emerging pathogens. RT-qPCR has been established as the reference technique for the diagnosis of SARS-CoV-2 infections.
Ane Rivas-Macho, Ane Sorarrain, José M. Marimón, Felipe Goñi-de-Cerio, Garbiñe Olabarria   +4 more
openaire   +3 more sources

Performance of colorimetric reverse transcription loop-mediated isothermal amplification as a diagnostic tool for SARS-CoV-2 infection during the fourth wave of COVID-19 in Thailand

International Journal of Infectious Diseases, 2022
: Background: COVID-19, which is caused by SARS-CoV-2 and its variants, poses an ongoing global threat, particularly in low-immunization coverage regions.
Thanyarat Promlek, Maytawan Thanunchai, Uraporn Phumisantiphong, Tonsan Hansirisathit, Chayanit Phuttanu, Sunisa Dongphooyao, Wipawee Thongsopa, Pornlada Nuchnoi   +7 more
doaj   +1 more source

Self-partitioning SlipChip for slip-induced droplet formation and human papillomavirus viral load quantification with digital LAMP [PDF]

, 2020
Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide, and persistent HPV infection can cause warts and even cancer.
Han, Xu, Ismagilov, Rustem F., Lai, Dongmei, Lyu, Weiyuan, Qu, Haijun, Shen, Feng, Wang, Qian, Yu, Mengchao, Yu, Ziqing   +8 more
core  

The current landscape of nucleic acid tests for filovirus detection. [PDF]

, 2018
Nucleic acid testing (NAT) for pathogenic filoviruses plays a key role in surveillance and to control the spread of infection. As they share clinical features with other pathogens, the initial spread of these viruses can be misdiagnosed.
Agnandji, Agnandji, Al-Soud, Al-Soud, Andrew D. Sails, Benzine, Bergqvist, Biek, Boyd, Cai, Carinelli, Chua, Chughtai, Cnops, David J. Clark, De Santis, Deen, Drosten, Duy, Dye, Ewer, FDA, Gay-Andrieu, Geisbert, Gibb, Greninger, Haddock, Hayman, Henao-Restrepo, Henry M. Staines, Huttner, Jayme, John Tyson, Kibuuka, Koehler, Kurosaki, Kurosaki, Kurosaki, Laing, Ledgerwood, Leroy, Leroy, Leski, Loftis, Lu, Mate, Milligan, Ogawa, Ogawa, Oloniniyi, Park, Pattyn, Pettitt, Pinsky, Pourrut, Pyankov, Quick, Raftery, Regules, Rieger, Rodriguez, Rossi, Sanchez, Sanjeev Krishna, Schrader, Semper, Shah, Southern, Spengler, Swanepoel, Tapia, Towner, Towner, Trombley, Varkey, Waxman, Weidmann, Weingartl, Weller, WHO, WHO, Wong, Woolhouse, Xu, Zheng, Zhu   +85 more
core   +1 more source