Results 51 to 60 of about 2,171,089 (338)

BREAST CANCER TYPING USING RT-PCR ASSAY

open access: yesСибирский онкологический журнал, 2019
Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko   +6 more
doaj   +1 more source

Optimisation of COVID‐19 diagnostic pathways in acute hospital admissions to prevent nosocomial transmission

open access: yesThe Clinical Respiratory Journal, 2022
Introduction In the management of acute hospital admissions during the COVID‐19 pandemic, safe patient cohorting depends on robust admission diagnostic strategies.
Robert Livingstone   +6 more
doaj   +1 more source

Screening of the H69 and V70 deletions in the SARS-CoV-2 spike protein with a RT-PCR diagnosis assay reveals low prevalence in Lyon, France

open access: yesmedRxiv, 2020
The H69 and V70 deletions in SARS-CoV-2 spike protein have been detected in mink and human infections. We found that these deletions resulted in a false negative result for the spike target of a commercial RT-PCR assay.
A. Bal   +18 more
semanticscholar   +1 more source

RT-PCR for NoV v1 [PDF]

open access: yes, 2018
This assay amplified the partial VP1 gene (Region C) of the norovirus genome. The yield products could be sequenced.
Hengyun Guan   +3 more
openaire   +2 more sources

Modeling and analysis of competitive RT-PCR [PDF]

open access: yesNucleic Acids Research, 1998
The present studies demonstrate a theoretical and practical framework for the accurate quantitation of gene expression in RNA extracted from microscopic tissue samples. The approaches are developed around competitive RT-PCR techniques. Assay performance has been examined and validated at both the RT and PCR steps.
Peter J. Oefner   +5 more
openaire   +3 more sources

Estimating the false-negative test probability of SARS-CoV-2 by RT-PCR

open access: yesEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020
Background Reverse-transcription PCR (RT-PCR) assays are used to test for infection with the SARS-CoV-2 virus. RT-PCR tests are highly specific and the probability of false positives is low, but false negatives are possible depending on swab type and ...
Paul S. Wikramaratna   +3 more
semanticscholar   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

open access: yesFEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi   +5 more
wiley   +1 more source

West Nile Virus Lineage 2 in Free-Living Corvus cornix Birds in Poland

open access: yesTropical Medicine and Infectious Disease, 2023
The summer temperatures recorded in Poland in 2022 were among the highest in over 30 years and, combined with higher-than-expected rainfall, gave the impression of an almost tropical climate.
Jowita S. Niczyporuk   +6 more
doaj   +1 more source

ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling

open access: yesFEBS Letters, EarlyView.
In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li   +3 more
wiley   +1 more source

At what times during infection is SARS-CoV-2 detectable and no longer detectable using RT-PCR-based tests? A systematic review of individual participant data

open access: yesBMC Medicine, 2020
Background Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral ribonucleic acid (RNA) using reverse transcription polymerase chain reaction (RT-PCR) are pivotal to detecting current coronavirus disease (COVID-19) and duration of ...
S. Mallett   +19 more
semanticscholar   +1 more source

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