Results 1 to 10 of about 2,171,365 (292)

Rapid sample delivery for megahertz serial crystallography at X-ray FELs. [PDF]

IUCrJ, 2018
Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements.
Wiedorn MO, Awel S, Morgan AJ, Ayyer K, Gevorkov Y, Fleckenstein H, Roth N, Adriano L, Bean R, Beyerlein KR, Chen J, Coe J, Cruz-Mazo F, Ekeberg T, Graceffa R, Heymann M, Horke DA, Knoška J, Mariani V, Nazari R, Oberthür D, Samanta AK, Sierra RG, Stan CA, Yefanov O, Rompotis D, Correa J, Erk B, Treusch R, Schulz J, Hogue BG, Gañán-Calvo AM, Fromme P, Küpper J, Rode AV, Bajt S, Kirian RA, Chapman HN.   +37 more
europepmc   +10 more sources

Possibilities for serial femtosecond crystallography sample delivery at future light sources. [PDF]

Struct Dyn, 2015
Serial femtosecond crystallography (SFX) uses X-ray pulses from free-electron laser (FEL) sources that can outrun radiation damage and thereby overcome long-standing limits in the structure determination of macromolecular crystals.
Chavas LM, Gumprecht L, Chapman HN.
europepmc   +6 more sources

3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers. [PDF]

IUCrJ, 2023
X-ray free-electron lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial and temporal resolution. A principal challenge in this pursuit involves the delivery of samples to the X-ray interaction point in ...
Konold PE, You T, Bielecki J, Valerio J, Kloos M, Westphal D, Bellisario A, Varma Yenupuri T, Wollter A, Koliyadu JCP, Koua FHM, Letrun R, Round A, Sato T, Mészáros P, Monrroy L, Mutisya J, Bódizs S, Larkiala T, Nimmrich A, Alvarez R, Adams P, Bean R, Ekeberg T, Kirian RA, Martin AV, Westenhoff S, Maia FRNC.   +27 more
europepmc   +2 more sources

Recent Advances and Future Perspectives on Microfluidic Mix-and-Jet Sample Delivery Devices. [PDF]

Micromachines (Basel), 2021
The integration of the Gas Dynamic Virtual Nozzle (GDVN) and microfluidic technologies has proven to be a promising sample delivery solution for biomolecular imaging studies and has the potential to be transformative for a range of applications in ...
Hejazian M, Balaur E, Abbey B.
europepmc   +2 more sources

3D printed devices and infrastructure for liquid sample delivery at the European XFEL. [PDF]

J Synchrotron Radiat, 2022
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Vakili M, Bielecki J, Knoška J, Otte F, Han H, Kloos M, Schubert R, Delmas E, Mills G, de Wijn R, Letrun R, Dold S, Bean R, Round A, Kim Y, Lima FA, Dörner K, Valerio J, Heymann M, Mancuso AP, Schulz J.   +20 more
europepmc   +2 more sources

Sample Delivery Media for Serial Crystallography. [PDF]

Int J Mol Sci, 2019
X-ray crystallographic methods can be used to visualize macromolecules at high resolution. This provides an understanding of molecular mechanisms and an insight into drug development and rational engineering of enzymes used in the industry. Although conventional synchrotron-based X-ray crystallography remains a powerful tool for understanding molecular
Nam KH.
europepmc   +4 more sources

Strategies for sample delivery for femtosecond crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2019
Highly efficient data-collection methods are required for successful macromolecular crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is scarce, and the high peak brightness of each XFEL pulse destroys the exposed crystal volume. It is therefore necessary to combine diffraction images from a large number of crystals (
Martiel I, Müller-Werkmeister HM, Cohen AE.   +2 more
europepmc   +5 more sources

Programmed sample delivery on a pressurized paper. [PDF]

Biomicrofluidics, 2014
This paper reports a method to control the fluid flow in paper-based microfluidic devices simply by pressing over the channel surface of paper, thereby decreasing the pore size and permeability of a non-woven polypropylene sheet. As a result, fluid resistance is increased in the pressed region and causes flow rate to decrease.
Shin JH, Park J, Kim SH, Park JK.
europepmc   +5 more sources

A fixed-target platform for serial femtosecond crystallography in a hydrated environment [PDF]

IUCrJ, 2020
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while ...
M. L. Shelby, D. Gilbile, T. D. Grant, C. Seuring, B. W. Segelke, W. He, A. C. Evans, T. Pakendorf, P. Fischer, M. S. Hunter, A. Batyuk, M. Barthelmess, A. Meents, M. A. Coleman, T. L. Kuhl, M. Frank   +15 more
doaj   +3 more sources

Liquid sample delivery techniques for serial femtosecond crystallography. [PDF]

Philos Trans R Soc Lond B Biol Sci, 2014
X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at ...
Weierstall U.
europepmc   +4 more sources