STAR Protocols, 2022 Summary: Many cellular processes require the activities of complex molecular machines composed of several protein subunits. Insights into these systems can be gained by isolation of protein complexes followed by in vitro analyses determining the identity,
Lubos Cipak +7 more
doaj +2 more sources
Mitotic Phosphorylation of Swi6/HP1 Regulates Its Chromatin Binding and Chromosome Segregation [PDF]
The FASEB Journal, Volume 39, Issue 21, 15 November 2025.Heterochromatin protein 1 (HP1) plays an important role in heterochromatin assembly. HP1 proteins undergo additional phosphorylation during mitosis. However, the functional significance of the mitotic phosphorylation of HP1 remains unclear. We identified mitotic phosphorylation sites within fission yeast Swi6 and showed that this phosphorylation is ...
Yuriko Yoshimura +8 more
wiley +2 more sources
Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe [PDF]
Chromosoma, 2006 In fission yeast, meiotic prophase nuclei develop structures known as linear elements (LinEs), instead of a canonical synaptonemal complex. LinEs contain Rec10 protein. While Rec10 is essential for meiotic recombination, the precise role of LinEs in this process is unknown.
Lorenz, A. +3 more
openaire +5 more sources
Characterisation of the SUMO-like domains of Schizosaccharomyces pombe Rad60 [PDF]
, 2010 The S. pombe Rad60 protein is required for the repair of DNA double strand breaks, recovery from replication arrest, and is essential for cell viability.
Anja-Katrin Bielinsky +5 more
core +41 more sources
Protocol for development and use of spike-in control for chromatin immunoprecipitation of chromatin-binding proteins in Schizosaccharomyces pombe [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) assays provide quantitative information about the genomic localization of chromatin-binding proteins; however, their sensitivity is limited by several technical variables.
Jasbeer S. Khanduja, Mo Motamedi
doaj +2 more sources
Protocol for the analysis of proteasome activity, assembly state, and composition in fission yeast extracts using native gels [PDF]
STAR ProtocolsSummary: The proteasome is a macromolecular complex responsible for degrading short-lived or damaged proteins. Proteasome analysis presents challenges due to its high molecular weight and low stability.
Gabriel Ruiz-Romero +2 more
doaj +2 more sources
Mechanical Coupling With the Nuclear Envelope Shapes the Schizosaccharomyces pombe Mitotic Spindle [PDF]
Cytoskeleton, Volume 83, Issue 1, Page 40-52, January 2026.ABSTRACT The fission yeast Schizosaccharomyces pombe divides via closed mitosis, meaning that spindle elongation and chromosome segregation transpire entirely within the closed nuclear envelope. Both the spindle and nuclear envelope must undergo shape changes and exert varying forces on each other during this process.
Marcus A. Begley +8 more
wiley +2 more sources
Characterization of the Schizosaccharomyces pombe Cdk9/Pch1 Protein Kinase [PDF]
Journal of Biological Chemistry, 2003 Yi Pei, Stewart Shuman
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Elementary Protein Analysis in Schizosaccharomyces pombe [PDF]
Cold Spring Harbor Protocols, 2017 Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide ...
Ágnes Grallert, Iain Hagan
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The Schizosaccharomyces pombe PPR protein Ppr10 associates with a novel protein Mpa1 and acts as a mitochondrial translational activator [PDF]
Nucleic Acids Research, 2017 Ying Huang
exaly +2 more sources