Interaction Between Rec8 and Mis4 Is Required for Axis-Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis. [PDF]
We identified a Mis4 cohesin loader mutant that is specifically defective in chromatin axis formation and homologous recombination while retaining normal sister chromatid cohesion. The mis4‐LR mutant, carrying the L1150S and R1159G amino acid substitutions, exhibited reduced interaction between Mis4 and Rec8, suggesting a meiosis‐specific role for the ...
Sakuno T +3 more
europepmc +2 more sources
Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. [PDF]
Telomeres of nuclear chromosomes are usually composed of an array of tandemly repeated sequences that are recognized by specific Myb domain containing DNA-binding proteins (telomere-binding proteins, TBPs).
Regina Sepsiova +8 more
doaj +3 more sources
CSL proteins of Schizosaccharomyces pombe [PDF]
The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in ...
Převorovský, Martin
core +7 more sources
Protocol for the analysis of proteasome activity, assembly state, and composition in fission yeast extracts using native gels [PDF]
Summary: The proteasome is a macromolecular complex responsible for degrading short-lived or damaged proteins. Proteasome analysis presents challenges due to its high molecular weight and low stability.
Gabriel Ruiz-Romero +2 more
doaj +2 more sources
A cDNA of Schizosaccharomyces pombe encoding a homologue of DnaJ-like protein
A Schizosaccharomyces pombe homologue, Psi, was cloned from Schizosaccharomyces pombe cDNA library. Deduced amino acid sequence of the cDNA has 55% sequence homology with the Saccharomyces cerevisiae Sis1 protein and contains the structural features of a family of DnaJ proteins.
S K, Park, S K, Chon, H S, Yoo
openaire +3 more sources
Objective: Metformin, a well-known anti-diabetic drug and a caloric restriction mimetic, seems to attenuate aging through myriad cellular processes, wherein most of its mode of action is still elusive.
Çağatay Tarhan +2 more
doaj +1 more source
Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics. [PDF]
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited.
Bassem Al-Sady +4 more
doaj +1 more source
Protein prenylation in Schizosaccharomyces pombe [PDF]
S. pombe is shown to be a powerful system for studies concerning attachment or polyisoprenoid moieties to proteins, due to its ability to take up exogenous mevalonic acid efficiently. The fission yeast can take up about 5% of the exogenously added mevalonic acid and incorporate ∼10% of this into protein.
Giannakouros, Thomas +2 more
openaire +2 more sources
Budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe are good models for heterochromatin study. In S. pombe, H3K9 methylation and Swi6, an ortholog of mammalian HP1, lead to heterochromatin formation. However, S.
Junsoo Oh +3 more
doaj +1 more source
Explaining lengths and shapes of yeast by scaling arguments. [PDF]
Lengths and shapes are approached in different ways in different fields: they serve as a read-out for classifying genes or proteins in cell biology whereas they result from scaling arguments in condensed matter physics.
Daniel Riveline
doaj +1 more source

