Results 61 to 70 of about 45,524 (260)

Physical Interaction between Recombinational Proteins Rhp51 and Rad22 in Schizosaccharomyces pombe [PDF]

Journal of Biological Chemistry, 2002
In eukaryotes, Rad51 and Rad52 are two key components of homologous recombination and recombinational repair. These two proteins interact with each other. Here we investigated the role of interaction between Rhp51 and Rad22, the fission yeast homologs of Rad51 and Rad52, respectively, on the function of each protein.
Woo Jae, Kim, Eon Joo, Park, Hyojin, Lee, Rho Hyun, Seong, Sang Dai, Park   +4 more
openaire   +2 more sources

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, EarlyView.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

Post-translational processing of Schizosaccharomyces pombe YPT proteins.

Journal of Biological Chemistry, 1992
ras proteins are post-translationally processed at their carboxyl-terminal CAAX motif by a triplet of modifications: prenylation of C with farnesyl, proteolytic trimming of AAX, and carboxyl-methylation. These modifications co-operate with palmitoylation of nearby sites or a polybasic region to target plasma membrane localization.
C M, Newman, T, Giannakouros, J F, Hancock, E H, Fawell, J, Armstrong, A I, Magee   +5 more
openaire   +2 more sources

Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei [PDF]

BIO-PROTOCOL, 2018
DNA damage repair proteins form foci in response to DNA damaging agents. The efficiency and integrity of the DNA repair pathway of a particular eukaryotic (mutant) strain is usually determined by the number of foci formed compared with their wild-type counterpart.
Lim, Kim Kiat, Chen, Ee Sin
openaire   +3 more sources

Role of Schizosaccharomyces pombe RecQ homolog recombination and checkpoint genes in UV Damage tolerance [PDF]

, 1997
The cellular responses to DNA damage are complex and include direct DNA repair pathways that remove the damage and indirect damage responses which allow cells to survive DNA damage that has not been, or cannot be, removed.
Carr, A M, Lindsay, H D, Munday, C A, Murray, J M   +3 more
core   +3 more sources

Global Proteome Turnover Analyses of the Yeasts S. cerevisiae and S. pombe

Cell Reports, 2014
Summary: How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant but ...
Romain Christiano, Nagarjuna Nagaraj, Florian Fröhlich, Tobias C. Walther   +3 more
doaj   +1 more source

Inter-species complementation of the translocon beta subunit requires only its transmembrane domain. [PDF]

PLoS ONE, 2008
In eukaryotes, proteins enter the secretory pathway through the translocon pore of the endoplasmic reticulum. This protein translocation channel is composed of three major subunits, called Sec61alpha, beta and gamma in mammals. Unlike the other subunits,
Alexandre Leroux, Luis A Rokeach
doaj   +1 more source

DNA damage repair proteins across the Tree of Life

iScience, 2023
Summary: Genome maintenance is orchestrated by a highly regulated DNA damage response with specific DNA repair pathways. Here, we investigate the phylogenetic diversity in the recognition and repair of three well-established DNA lesions, primarily ...
Emily Nischwitz, Vivien A.C. Schoonenberg, Albert Fradera-Sola, Mario Dejung, Olga Vydzhak, Michal Levin, Brian Luke, Falk Butter, Marion Scheibe   +8 more
doaj   +1 more source

Synthetic biology approaches to generate temperature‐sensitive alleles for the Sterile Insect Technique

Insect Science, EarlyView.
Abstract The Sterile Insect Technique (SIT) is an environmentally friendly, sustainable pest control approach, which uses large‐scale releases of sterile insects to suppress or eradicate target populations through infertile matings. The efficiency of SIT is enhanced by male‐only releases requiring genetic sexing strains (GSSs) that are classically ...
Chun Yin Leung, Ernst A. Wimmer, Hassan M. M. Ahmed   +2 more
wiley   +1 more source

Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe. [PDF]

PLoS ONE, 2016
Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the ...
Dirk Landgraf, Dann Huh, Erinc Hallacli, Susan Lindquist   +3 more
doaj   +1 more source