Results 61 to 70 of about 27,508 (229)

Rapid regulation of protein activity in fission yeast

, 2014
Background: The fission yeast Schizosaccharomyces pombe is widely-used as a model organism for the study of a broad range of eukaryotic cellular processes such as cell cycle, genome stability and cell morphology. Despite the availability of extensive set
Sharom, Jeffrey R., Boe, Cathrine A., Tyers, Michael D., Boye, Erik, Kearsey, Stephen, MacNeill, Stuart Andrew, Pai, Chen-Chun, Skjolberg, Henriette C., Grallert, Beata, Garcia, Ignacio   +9 more
core   +1 more source

S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules [PDF]

, 2012
The kinesins-8 were originally thought to be microtubule depolymerases, but are now emerging as more versatile catalysts of microtubule dynamics. We show here that S.
Muriel Erent (185011), Douglas R Drummond, Cross, R. A., Douglas R. Drummond (185016), Erent, Muriel, Cross Robert A., Drummond, Douglas R., Drummond Douglas R., Muriel Erent, Robert A. Cross (185019), Robert A Cross, Erent Muriel   +11 more
core   +1 more source

The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]

, 2005
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V., Hardwick, Kevin, Kadura, Sheila, Sazer, Shelley, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G   +6 more
core   +1 more source

Inter-species complementation of the translocon beta subunit requires only its transmembrane domain. [PDF]

PLoS ONE, 2008
In eukaryotes, proteins enter the secretory pathway through the translocon pore of the endoplasmic reticulum. This protein translocation channel is composed of three major subunits, called Sec61alpha, beta and gamma in mammals. Unlike the other subunits,
Alexandre Leroux, Luis A Rokeach
doaj   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

RNA interference-mediated co-transcriptional gene silencing in fission yeast [PDF]

, 2012
In the last decade or so, RNA interference (RNAi) has gained unanticipated recognition in the fields of RNA biology and gene regulation. It exists in a wide variety of eukaryotic organisms, and various forms of RNAi are involved in diverse biological ...
Woolcock, Katrina Jane
core   +1 more source

Global Proteome Turnover Analyses of the Yeasts S. cerevisiae and S. pombe

Cell Reports, 2014
Summary: How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant but ...
Romain Christiano, Nagarjuna Nagaraj, Florian Fröhlich, Tobias C. Walther   +3 more
doaj   +1 more source

Monoallelic POLR3A Variants Cause Early‐Onset Peripheral Neuropathy

Annals of Neurology, EarlyView.
Objective Biallelic variants in genes encoding the RNA polymerase III complex (Pol III) cause a spectrum of neurological disorders primarily affecting the central nervous system. Monoallelic variants have been reported in the POLR3B subunit only, associated with neurodevelopmental disorder, epilepsy, and peripheral neuropathy.
Luiza L. P. Ramos, Jevin M. Parmar, Robin Wijngaard, Bianca R. Grosz, Tamas Lazar, Ligia Mateiu, Steve Vucic, Kishore R. Kumar, Dennis Yeow, Laura I. Rudaks, Lonneke de Boer, Annemarie de Vreugd, David A. Koolen, Thatjana Gardeitchik, Anita Cairns, Krishnan Iyengar, Fernando Kok, Fernanda Barbosa Figueiredo, Alzira Alves de Siqueira Carvalho, Luiz S. Mageste Barbosa, Rodrigo Rezende Arantes, Tyler Rehbein, Jordan E. Bontrager, Elizabeth P. Wood, Janet E. Sowden, Gavin Monahan, Meutia Kumaheri, Ivy Cuijt, Melina Ellis, Gonzalo Perez‐Siles, Elyshia McNamara, Ronald van Beek, Celine B. Meijers, Ivaylo Tournev, Stephan Zuchner, Shoshana J. Wodak, Clara D. M. van Karnebeek, Nigel Laing, Liana N. Semcesen, David A. Stroud, David N. Herrmann, Velina Guergueltcheva, Marina L. Kennerson, Machteld M. Oud, Gianina Ravenscroft, Ayse Candayan, Albena Jordanova   +46 more
wiley   +1 more source

Improved tools for efficient mapping of fission yeast genes:identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 [PDF]

, 2008
Fission yeast genes identified in genetic screens are usually cloned by transformation of mutants with plasmid libraries. However, for some genes this can be difficult, and positional cloning approaches are required.
Sawin, Kenneth E, Watt, Stephen, Bähler, Jürg, Anders, Andreas   +3 more
core   +1 more source

Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe. [PDF]

PLoS ONE, 2016
Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the ...
Dirk Landgraf, Dann Huh, Erinc Hallacli, Susan Lindquist   +3 more
doaj   +1 more source