Results 121 to 130 of about 670,402 (263)

SDS-PAGE

open access: yesSDS-PAGE
3章 単離・精製・活性測定 6. タンパク質 a 調製した生体試料のタンパク質組成を解析する際などに,最初に行われる最も基礎的な実験のひとつがSDS-PAGEであろう.近年のプロテオミクス解析に於いて広く用いられる等電点二次元電気泳動でも,二次元目にはSDS-PAGEが行われるのが一般的である.比較的容易に行うことができるSDS-PAGEであるが,手元の生体試料に含まれているタンパク質群の中にある重要なタンパク質を見落とさないために留意しておくと良いことがある.本稿では,4種類のSDS-PAGE系を比較しながら,それぞれの実験方法を簡単に記述する. SDS-PAGE is one of the very basic techniques for the protein analysis.
openaire  

SDS-PAGE Cracking Buffer v1

open access: yes, 2016
Recipe to make 10ml of 2x stock
openaire   +1 more source

Expanding Chemical Space of Nucleic Acid Nanoparticles for Tunable Antiviral‐Like Immunomodulatory Responses and Potent Adjuvant Activity

open access: yesAdvanced Functional Materials, EarlyView.
We introduce a nucleic acid nanoparticle (NANP) platform designed to be rrecognized by the human innate immune system in a regulated manner. By changing chemical composition while maintaining constant architectural parameters, we identify key determinants of immunorecognition enabling the rational design of NANPs with tunable immune activation profiles
Martin Panigaj   +21 more
wiley   +1 more source

Spleen‐Targeting Biomimetic Hybrid Nanocarriers for Systemic Immune Reprogramming in Colitis: RBC Membrane Vesicle‐Fused Lipid Nanoparticles

open access: yesAdvanced Functional Materials, EarlyView.
A spleen‐targeting hybrid nanoplatform (RBCMV‐LNP‐RP) harnessing erythrophagocytosis mimics senescent red blood cell clearance to achieve spleen‐specific delivery of rapamycin. This biomimetic system enables selective accumulation in splenic macrophages, orchestrating systemic immune reprogramming and promoting mucosal healing in an inflammatory bowel ...
Jun Kwon   +10 more
wiley   +1 more source

SDS-PAGE [PDF]

open access: yes, 2019
openaire   +1 more source

Generating Cell Surface Nucleated Hydrogels with an Artificial Membrane‐Binding Transglutaminase

open access: yesAdvanced Functional Materials, EarlyView.
Cell‐based therapies require advanced strategies to enhance cell delivery and bioactivity. Cell membrane engineering offers an avenue to impart new functions to delivered cells to boost their viability and function. Here, an artificial membrane‐binding transglutaminase is generated and biophysically characterized.
Rosalia Cuahtecontzi Delint   +6 more
wiley   +1 more source

Determination of yolk:white ratio of egg using SDS-PAGE. [PDF]

open access: yesFood Sci Biotechnol, 2020
Uysal RS, Acar-Soykut E, Boyaci IH.
europepmc   +1 more source

DNA‐Templated 2D Heterostructures as Phototriggered Dynamic Nanohybrids: From Releasing Molecular Loads to Controlling Enzyme Biocatalytic Function

open access: yesAdvanced Functional Materials, EarlyView.
DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen   +3 more
wiley   +1 more source

Synthetic Nanobiology Actuated Lipometabolic Cell Factory for Autologous Tumor Immunotherapy

open access: yesAdvanced Functional Materials, EarlyView.
FA plays a crucial role in the interaction between tumor cells and the tumor microenvironment, especially for the immune response. A biocatalytic immunoenhancement strategy is developed to boost antitumor immunity by FA metabolic orientation to ceramide. Through the design of this delicate catalytic immunoenhancement strategy, the synthetic nanobiology
Shoujie Zhao   +8 more
wiley   +1 more source

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