Results 81 to 90 of about 61,645 (272)

Microfluidic Modeling of Macrophage‐Induced Cardiac Inflammation Using NF‐κB Reporter Cardiomyocytes

Advanced Healthcare Materials, EarlyView.
A cardiac inflammation model was developed to enable real‐time analysis of macrophage‐cardiomyocyte interactions using NF‐κB‐reporter cardiomyocytes and a microfluidic co‐culture system. A MATLAB‐based model was implemented to optimize cell density and medium resupply based on TNFα diffusion kinetics. Activation thresholds and stress responses in NF‐κB‐
Chao Liu, Xi Rui Qiang Lu, Seoyoung Jeong, Douglas H. Wu, Rodrigo A. Somoza, Parameswaran Ramakrishnan, Samuel E. Senyo   +6 more
wiley   +1 more source

Quantitative secretome analysis establishes the cell type-resolved mouse brain secretome [PDF]

, 2020
AbstractTo understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the high-performance secretome-protein-enrichment-with-click-sugars method (hiSPECS). To demonstrate its
Johanna Tüshaus, Stephan A. Müller, Evans Sioma Kataka, Jan Zaucha, Laura Sebastian Monasor, Minhui Su, Gökhan Güner, Georg Jocher, Sabina Tahirovic, Dmitrij Frishman, Mikael Simons, Stefan F. Lichtenthaler   +11 more
openaire   +1 more source

A Synovium‐on‐Chip Platform to Study Multicellular Interactions in Arthritis

Advanced Healthcare Materials, EarlyView.
The Synovium‐on‐Chip comprises a thin microporous PDMS membrane to support co‐culture of fibroblast‐like synoviocytes (FLS), THP‐1‐derived macrophages, and endothelial cells, enabling real‐time analysis of synovial‐vascular interactions. FLS migration through the pores drives endothelial remodeling, while TNF‐α stimulation induces robust inflammatory ...
Laurens R. Spoelstra, Fleur R. Semmekrot, Nuno Araújo‐Gomes, Daniël Wijnperle, Monique M. A. Helsen, Martijn H.J. Van Den Bosch, Loes. I. Segerink, Séverine Le Gac, Marcel Karperien   +8 more
wiley   +1 more source

Secret3D Workflow for Secretome Analysis

STAR Protocols, 2020
Secretome analysis is crucial to unravel extracellular processes. However, secreted proteins are difficult to detect in mass-spectrometry-based analysis due to contamination of serum proteins deriving from cell culture media and to high glycosylation, which hampers tryptic digestion. Secret3D workflow is an optimized protocol for the global analysis of
Vittoria Matafora, Angela Bachi
openaire   +3 more sources

Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae. [PDF]

, 2017
The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect different hosts, including humans and plants.
Almeida, Nalvo F, Assis, Renata de AB, Dandekar, Abhaya M, Diaz-Caballero, Julio, Digiampietri, Luciano Antonio, Felestrino, Érica B, Goulart, Luiz Ricardo, Guttman, David S, Moreira, Leandro Marcio, Nascimento, Rafael, Patané, José SL, Polloni, Lorraine Cristina, Setubal, João C, Thakur, Shalabh, Zaini, Paulo A   +14 more
core   +2 more sources

A Superhydrophobic 3D Cell Culture System Reveals the Mechanobiological Role of Cancer‐Associated Fibroblasts in Prostate Cancer Metastasis

Advanced Healthcare Materials, EarlyView.
A superhydrophobic 3D cell culture platform enables rapid and consistent formation of heterotypic tumor–stroma clusters, and reveals how physiological shear conditions influence metastatic signaling. Mechanical stimulation of cancer‐associated fibroblasts promotes sustained cytokine secretion and survival‐promoting pathways, showing how multicellular ...
Alexandria T. Carter, Abigail R. Fabiano, Ehsan Aalaei, Jason Deng, Dakota E. Rostant, Michael R. King   +5 more
wiley   +1 more source

RETRACTED ARTICLE: Human fetal skin-derived stem cell secretome enhances radiation-induced skin injury therapeutic effects by promoting angiogenesis

Stem Cell Research & Therapy, 2019
Background Radiation dermatitis is a refractory skin injury caused by radiotherapy. Human fetal skin-derived stem cell (hFSSC) is a preferable source for cell therapy and skin tissue regeneration.
Xiaoli Rong, Jiannan Li, Yanyan Yang, Liyan Shi, Tiechao Jiang   +4 more
doaj   +1 more source

Hepatocyte growth factor, a key tumor-promoting factor in the tumor microenvironment [PDF]

, 2017
The tumor microenvironment plays a key role in tumor development and progression. Stromal cells secrete growth factors, cytokines and extracellular matrix proteins which promote growth, survival and metastatic spread of cancer cells.
Galemmo, Robert, Janetka, James, Klampfer, Lidija, Owusu, Benjamin Yaw   +3 more
core   +2 more sources

3D Soft Hydrogels Induce Human Mesenchymal Stem Cells “Deep” Quiescence

Advanced Healthcare Materials, EarlyView.
Three‐dimensional soft hydrogels mimicking the bone marrow niche induce deep quiescence in human mesenchymal stem cells. Unlike 2D culture, 3D matrices halt proliferation, regulate cell‐cycle and quiescence markers, and downregulate mTORC1 signaling, preserving stem cell phenotype and therapeutic potential ex vivo.
David Boaventura Gomes, Timo Rademakers, Ana Filipa Henriques Lourenço, Andrea Calore, Clarissa Tomasina, Denis van Beurden, Jinjie Venema, Bryan Chömpff, Hong Liu, Nicole Bouvy, Sandra Camarero‐Espinosa, Lorenzo Moroni   +11 more
wiley   +1 more source

Argonaute2 Regulates the Pancreatic β-Cell Secretome [PDF]

Molecular & Cellular Proteomics, 2013
Argonaute2 (Ago2) is an established component of the microRNA-induced silencing complex. Similar to miR-375 loss-of-function studies, inhibition of Ago2 in the pancreatic β-cell resulted in enhanced insulin release underlining the relationship between these two genes.
S. G. Tattikota, M. D. Sury, T. Rathjen, H.-H. Wessels, A. K. Pandey, X. You, C. Becker, W. Chen, M. Selbach, M. N. Poy   +9 more
openaire   +2 more sources