Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec translocon [PDF]
The EMBO Journal, 2023 The Sec translocon is a highly conserved membrane assembly for polypeptide transport across, or into, lipid bilayers. In bacteria, secretion through the core channel complex—SecYEG in the inner membrane—is powered by the cytosolic ATPase SecA.
Joel A Crossley +7 more
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Voltage Sensing in Bacterial Protein Translocation [PDF]
Biomolecules, 2020 The bacterial channel SecYEG efficiently translocates both hydrophobic and hydrophilic proteins across the plasma membrane. Translocating polypeptide chains may dislodge the plug, a half helix that blocks the permeation of small molecules, from its ...
Denis G. Knyazev +5 more
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A unifying mechanism for protein transport through the core bacterial Sec machinery [PDF]
Open Biology, 2023 Encapsulation and compartmentalization are fundamental to the evolution of cellular life, but they also pose a challenge: how to partition the molecules that perform biological functions—the proteins—across impermeable barriers into sub-cellular ...
William J. Allen, Ian Collinson
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Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins. [PDF]
PLoS Computational BiologyAtomic Force Microscopy (AFM) can create images of biomolecules under near-native conditions but suffers from limited lateral resolution due to the finite AFM tip size and recording frequency.
Creighton M Lisowski +2 more
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Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation [PDF]
International Journal of MicrobiologyWith Bacillus species, about 30% of extracellular proteins are translocated through the cytoplasmic membrane, coordinated by the Sec translocase. This system mainly consists of the cytoplasmic ATPase SecA and the membrane-embedded SecYEG channel.
Frédéric Yannick Okouakoua +5 more
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Charge Neutralization During Peptide Transport in the Bacterial SecYEG Translocon [PDF]
BiomoleculesThe driving force behind protein translocation across the cell membrane is not yet fully understood. In bacteria, there is an electrochemical potential across the cell membrane, which can interact with charged residues in the translocation substrate.
Laura Nübl +2 more
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AFM observation of protein translocation mediated by one unit of SecYEG-SecA complex [PDF]
Nature CommunicationsProtein translocation across cellular membranes is an essential and nano-scale dynamic process. In the bacterial cytoplasmic membrane, the core proteins in this process are a membrane protein complex, SecYEG, corresponding to the eukaryotic Sec61 complex,
Yui Kanaoka +11 more
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Dynamic action of the Sec machinery during initiation, protein translocation and termination [PDF]
eLife, 2018 Protein translocation across cell membranes is a ubiquitous process required for protein secretion and membrane protein insertion. In bacteria, this is mostly mediated by the conserved SecYEG complex, driven through rounds of ATP hydrolysis by the ...
Tomas Fessl +9 more
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B. subtilis Sec and Srp Systems Show Dynamic Adaptations to Different Conditions of Protein Secretion [PDF]
CellsSecA is a widely conserved ATPase that drives the secretion of proteins across the cell membrane via the SecYEG translocon, while the SRP system is a key player in the insertion of membrane proteins via SecYEG. How SecA gains access to substrate proteins
Svenja M. Fiedler, Peter L. Graumann
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Cell Reports, 2023 Summary: Signal-sequence-dependent protein targeting is essential for the spatiotemporal organization of eukaryotic and prokaryotic cells and is facilitated by dedicated protein targeting factors such as the signal recognition particle (SRP).
Pinku Sarmah +8 more
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