Results 91 to 100 of about 3,879 (192)
ATPase and GTPase Tangos Drive Intracellular Protein Transport [PDF]
, 2016 The GTPase superfamily of proteins provides molecular switches to regulate numerous cellular processes. The ‘GTPase switch’ paradigm, in which external regulatory factors control the switch of a GTPase between ‘on’ and ‘off’ states, has been used to ...
Shan, Shu-ou
core +1 more source
Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon [PDF]
, 2017 A modular approach for balanced overexpression of recombinant multiprotein complexes in E. coli is described, with the prokaryotic protein secretase/insertase complex, the SecYEG-SecDFYajC-YidC holotranslocon (HTL), used as an example. This procedure has been implemented here in the ACEMBL system.
Berger, Imre +4 more
openaire +4 more sources
SecYEG activates GTPases to drive the completion of cotranslational protein targeting [PDF]
Journal of Cell Biology, 2013 Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the translating ribosome or actively regulates this targeting ...
Akopian, David +4 more
openaire +3 more sources
The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
eLife, 2018 Membrane proteins are difficult to work with due to their insolubility in aqueous solution and quite often their poor stability in detergent micelles. Here, we present the peptidisc for their facile capture into water-soluble particles.
Michael Luke Carlson +13 more
doaj +1 more source
Membranes, 2022 Proteins embedded in biological membranes perform essential functions in all organisms, serving as receptors, transporters, channels, cell adhesion molecules, and other supporting cellular roles.
Matthew J. Laurence +5 more
doaj +1 more source
The hessian blob algorithm : precise particle detection in atomic force microscopy imagery [PDF]
, 2018 "Received: 20 October 2017; Accepted: 29 December 2017; Published online: 17 January 2018."Imaging by atomic force microscopy (AFM) offers high-resolution descriptions of many biological systems; however, regardless of resolution, conclusions drawn from ...
Chada, Nagaraju +4 more
core +2 more sources
eLife, 2019 Transport of proteins across membranes is a fundamental process, achieved in every cell by the ‘Sec’ translocon. In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion.
Robin A Corey +8 more
doaj +1 more source
The contribution of mRNA targeting to spatial protein localization in bacteria
The FEBS Journal, Volume 291, Issue 21, Page 4639-4659, November 2024.mRNA‐based targeting serves as a back‐up strategy for the canonical signal sequence‐based protein targeting in bacteria. While signal sequence‐based targeting depends on dedicated protein‐targeting factors, mRNAs encoding inner membrane proteins can bind directly to protein translocases.
Wenkang Shang +4 more
wiley +1 more source
Deregulation of the SecYEG Translocation Channel upon Removal of the Plug Domain [PDF]
Journal of Biological Chemistry, 2007 Previous studies have shown that the SecY plug is displaced from the center of the SecYEG channel during polypeptide translocation. The structural and functional consequences of the deletion of the plug are now examined. Both in vivo and in vitro observations indicate that the plug domain is not essential to the function of the translocon.
Maillard, Antoine P. +4 more
openaire +4 more sources
eLife, 2016 The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the
William John Allen +7 more
doaj +1 more source