Results 101 to 110 of about 3,879 (192)

Composition and activity of the non-canonical Gram-positive SecY2 complex [PDF]

, 2016
The accessory Sec system in Streptococcus gordonii DL1 is a specialized export system that transports a large serine-rich repeat protein, Hsa, to the bacterial surface.
Bandara, Mikaila, Blocker, Ariel J., Collinson, Ian, Corey, Robin A., Jenkinson, Howard F., Martin, Remy, Skehel, J. Mark   +6 more
core   +2 more sources

Redox regulation in chloroplast thylakoid lumen: The pmf changes everything, again

Plant, Cell &Environment, Volume 47, Issue 8, Page 2749-2765, August 2024.
Abstract Photosynthesis is the foundation of life on Earth. However, if not well regulated, it can also generate excessive reactive oxygen species (ROS), which can cause photodamage. Regulation of photosynthesis is highly dynamic, responding to both environmental and metabolic cues, and occurs at many levels, from light capture to energy storage and ...
Donghee Hoh, John E. Froehlich, David M. Kramer   +2 more
wiley   +1 more source

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts [PDF]

, 2016
Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states.
A Basters, A Kriz, AB Rode, AJ Jakobi, B Geering, B Weiss, BM Olivera, C Bieniossek, C Bieniossek, C Romier, C Schaffitzel, CP Chou, CV Robinson, D Busso, D Sari, D Schelshorn, DG Gibson, DJ Pitera, DN Gopaul, F Guo, F Leinert, G Bartani, G Gosset, G Kellenberger, H Alper, H Ye, HO Smith, J Komar, J Quan, J Torella, JA Chemler, JH Park, K Ballmer-Hofer, KB Mullis, LF Estrozi, LR Jarboe, LS Vijayachandran, M Folcher, M Gellert, M Haffke, M Haffke, M Meselson, MCY Chang, ML Diebold, ML Gefter, MZ Li, N Zheng, NR Cozzarelli, O Loeffelholz Von, PE Nisson, PJA Cock, R Patnaik, R Vincentelli, RJ Schulze, S Ausländer, S Cunna, S Linn, S Trowitzsch, S Trowitzsch, SC Almo, SE Luria, SJ Berríos-Rivera, SK Lee, SY Lee, T Maniatis, TC Terwilliger, VC Bode, W Abdulrahman, W Weber, WW Metcalf, Y Nie, Y Zhang   +71 more
core   +3 more sources

Bacterial Glycolipid Acting on Protein Transport Across Membranes

ChemBioChem, Volume 25, Issue 10, May 17, 2024.
In Escherichia coli inner membranes, diacylglycerol (DAG) and a glycolipid, named membrane protein integrase (MPIase), particularly influence membrane protein transport. This review explores the mechanisms of membrane protein integration mediated by these factors, and the methods and results of physicochemical analysis are presented.
Shoko Mori, Masafumi Shionyu, Keiko Shimamoto, Kaoru Nomura   +3 more
wiley   +1 more source

Comparison of Single and Multiple Turnovers of SecYEG in Escherichia coli

Journal of Bacteriology, 2020
Vastly different experimental techniques and conditions have been used to study export in E. coli . We demonstrated that altering experimental conditions can change the step that is observed during study. Investigators should consider specific experimental conditions when comparing data from different laboratories ...
Chunfeng Mao, Priya Bariya, Yuying Suo, Linda L. Randall   +3 more
openaire   +3 more sources

Shaping the lipid composition of bacterial membranes for membrane protein production

Microbial Cell Factories, 2019
Background The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production.
Kerstin Kanonenberg, Jorge Royes, Alexej Kedrov, Gereon Poschmann, Federica Angius, Audrey Solgadi, Olivia Spitz, Diana Kleinschrodt, Kai Stühler, Bruno Miroux, Lutz Schmitt   +10 more
doaj   +1 more source

Bacterial protein translocation requires only one copy of the SecY complex in vivo [PDF]

, 2012
The transport of proteins across the plasma membrane in bacteria requires a channel formed from the SecY complex, which cooperates with either a translating ribosome in cotranslational translocation or the SecA ATPase in post-translational translocation.
Park, Eunyong, Rapoport, Tom A.
core   +1 more source

Weak links: Advancing target‐based drug discovery by identifying the most vulnerable targets

Annals of the New York Academy of Sciences, Volume 1535, Issue 1, Page 10-19, May 2024.
Mycobacterium tuberculosis (TB) is the leading cause of death due to infectious disease. Developing new antibiotics that can shorten TB treatment time will require innovation in target‐based drug discovery in addition to phenotypic screens. In this review, we highlight a novel metric to prioritize essential targets: vulnerability.
Barbara Bosch, Michael A. DeJesus, Dirk Schnappinger, Jeremy M. Rock   +3 more
wiley   +1 more source

HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

eLife, 2019
The bacterial Sec translocon is a multi-protein complex responsible for translocating diverse proteins across the plasma membrane. For post-translational protein translocation, the Sec-channel – SecYEG – associates with the motor protein SecA to mediate ...
Zainab Ahdash, Euan Pyle, William John Allen, Robin A Corey, Ian Collinson, Argyris Politis   +5 more
doaj   +1 more source

Super‐resolution fluorescence microscopy for investigating bacterial cell biology

Molecular Microbiology, Volume 121, Issue 4, Page 646-658, April 2024.
Current developments in super‐resolution fluorescence microscopy are opening up new possibilities in molecular and cellular bacteriology. The latest applications are presented including the use of MINFLUX nanoscopy for research into secretion systems.
Alexander Carsten, Manuel Wolters, Martin Aepfelbacher   +2 more
wiley   +1 more source