Results 11 to 20 of about 3,879 (192)

The protein-conducting channel SecYEG [PDF]

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2004
In bacteria, the translocase mediates the translocation of proteins into or across the cytosolic membrane. It consists of a membrane embedded protein-conducting channel and a peripherally associated motor domain, the ATPase SecA. The channel is formed by
Driessen, AM, van der Does, C, Veenendaal, AKJ, Veenendaal, Andreas K.J.   +3 more
core   +13 more sources

The Dynamic SecYEG Translocon [PDF]

Frontiers in Molecular Biosciences, 2021
The spatial and temporal coordination of protein transport is an essential cornerstone of the bacterial adaptation to different environmental conditions.
Julia Oswald, Robert Njenga, Robert Njenga, Ana Natriashvili, Ana Natriashvili, Pinku Sarmah, Pinku Sarmah, Hans-Georg Koch   +7 more
doaj   +4 more sources

YidC and SecYEG form a heterotetrameric protein translocation channel [PDF]

Scientific Reports, 2017
The heterotrimeric SecYEG complex cooperates with YidC to facilitate membrane protein insertion by an unknown mechanism. Here we show that YidC contacts the interior of the SecY channel resulting in a ligand-activated and voltage-dependent complex with ...
Ilie Sachelaru, Lukas Winter, Denis G. Knyazev, Mirjam Zimmermann, Andreas Vogt, Roland Kuttner, Nicole Ollinger, Christine Siligan, Peter Pohl, Hans-Georg Koch   +9 more
doaj   +4 more sources

The TerC family metal chaperone MeeY enables surfactin export in Bacillus subtilis [PDF]

Journal of Bacteriology
TerC family proteins are widely conserved integral membrane proteins with functions related to metal transport. In Bacillus subtilis, the TerC proteins MeeF and MeeY play overlapping roles in the metalation of manganese-requiring membrane and ...
Bixi He, Ankita J. Sachla, Sadie B. Ruesewald, Daniel B. Kearns, John D. Helmann   +4 more
doaj   +2 more sources

MultiPassMerger: Automated data processing for multipass cyclic ion mobility HDX-MS. [PDF]

Protein Sci
Abstract Hydrogen/deuterium exchange‐mass spectrometry (HDX‐MS) is a powerful tool for studying protein structure and dynamics. As a bottom‐up LC‐MS technique, its success largely depends on peptide identifications made by peptide mapping prior to HDX measurements.
Duerr V, Griffiths D, Politis A.
europepmc   +2 more sources

SecYEG-mediated translocation in a model synthetic cell. [PDF]

Synth Biol (Oxf)
Abstract Giant unilamellar vesicles (GUVs) provide a powerful model compartment for synthetic cells. However, a key challenge is the incorporation of membrane proteins that allow for transport, energy transduction, compartment growth and division.
Schoenmakers LLJ, den Uijl MJ, Postma JL, van den Akker TAP, Huck WTS, Driessen AJM.   +5 more
europepmc   +6 more sources

The Multifunctional Preprotein Binding Domain of SecA. [PDF]

Chembiochem
SecA protein has evolved to perform multiple tasks. Its Preprotein Binding Domain (PBD) is a key module, adopting three conformational states. The PBD's inter‐ and intra‐protomeric interactions, along with biophysical data, reveal the influence of these states on the translocation process.
Giotas E, Aikaterini Kaplani S, Eleftheriadis N.   +2 more
europepmc   +2 more sources

High Efficacy of Rose Bengal in Reducing the Pathogenicity of <i>Escherichia coli</i> Isolated From Diarrheal Infections. [PDF]

Int J Microbiol
Diarrheal infections, a leading cause of global morbidity and mortality, are frequently attributed to pathogenic Escherichia coli strains. The rise of antibiotic resistance among these pathogens necessitates the exploration of alternative therapeutic agents.
Malanda CDB, Kayath CA, Nieko NPMD, Okouakoua FY, Lambi NN, Niangui DHK, Bissoko SPJ, Kinavouidi DJK.   +7 more
europepmc   +2 more sources

Interaction between glycolipid MPIase and proteinaceous factors during protein integration into the cytoplasmic membrane of E. coli

Frontiers in Molecular Biosciences, 2022
Protein integration into biomembranes is an essential biological phenomenon common to all organisms. While various factors involved in protein integration, such as SRP, SecYEG and YidC, are proteinaceous, we identified a glycolipid named MPIase (Membrane
Hanako Nishikawa, Katsuhiro Sawasato, Shoko Mori, Kohki Fujikawa, Kaoru Nomura, Keiko Shimamoto, Ken-Ichi Nishiyama   +6 more
doaj   +1 more source

Opening the SecYEG Protein Translocon [PDF]

Biophysical Journal, 2009
The Sec61/SecYEG translocon is the central component of the major biosynthetic pathway for membrane and secreted proteins. To incorporate membrane proteins into the endoplasmic reticulum membrane of eukaryotes (Sec61), or into the plasma membrane of prokaryotes (SecYEG), the translocon relies on the opening of a lateral helical gate formed by ...
Bondar, Ana Nicoleta, Tobias, Douglas J, White, Stephen H   +2 more
openaire   +2 more sources