Results 81 to 90 of about 3,879 (192)
Lipids Activate SecA for High Affinity Binding to the SecYEG Complex [PDF]
Journal of Biological Chemistry, 2016 Protein translocation across the bacterial cytoplasmic membrane is an essential process catalyzed predominantly by the Sec translocase. This system consists of the membrane-embedded protein-conducting channel SecYEG, the motor ATPase SecA, and the heterotrimeric SecDFyajC membrane protein complex.
Koch, Sabrina +7 more
openaire +3 more sources
Structure of the substrate-engaged SecA-SecY protein translocation machine. [PDF]
, 2019 The Sec61/SecY channel allows the translocation of many proteins across the eukaryotic endoplasmic reticulum membrane or the prokaryotic plasma membrane.
Catipovic, Marco +7 more
core
Structure of the SecY channel during initiation of protein translocation [PDF]
, 2014 Many secretory proteins are targeted by signal sequences to a protein-conducting channel, formed by prokaryotic SecY- or eukaryotic Sec61-complexes, and are translocated across the membrane during their synthesis1,2.
Akey, Christopher W. +7 more
core +1 more source
Defining the heterogeneous composition of Arabidopsis thylakoid membrane
The Plant Journal, Volume 121, Issue 3, February 2025.Significance Statement Thylakoid membrane of flowering plants exhibits extreme heterogeneity in function, biogenesis, assembly and repair, as well as in the localization of proteins and protein complexes in appressed grana, unstacked stroma lamellae (SL), connecting (CD) and curvature domains.
Andrea Trotta +5 more
wiley +1 more source
Protein Translocation: The Sec61/SecYEG Translocon Caught in the Act [PDF]
Current Biology, 2014 The Sec61/SecYEG complex mediates both the translocation of newly synthesized proteins across the membrane and the integration of transmembrane segments into the lipid bilayer. New cryo-electron microscopy studies show ribosome-channel complexes in action and reveal their repertoire of conformational states.
openaire +4 more sources
Molecular Mimicry of SecA and Signal Recognition Particle Binding to the Bacterial Ribosome
mBio, 2019 Bacteria execute a variety of protein transport systems for maintaining the proper composition of their different cellular compartments. The SecYEG translocon serves as primary transport channel and is engaged in transporting two different substrate ...
Lara Knüpffer +5 more
doaj +1 more source
Escherichia coli EHEC Germany outbreak preliminary functional annotation using BG7 system [PDF]
, 2011 We have annotated the European outbreak E. coli EHEC genome sequenced by BGI (6-2-2011) and assembled with MIRA by Nick Loman (6-2-2011 ). Our system BG7, Bacterial Genome annotation of Era7 Bioinformatics, predicts ORFs and annotates them based on ...
Eduardo Pareja +4 more
core +2 more sources
Journal of Molecular Recognition, Volume 38, Issue 1, January 2025.ABSTRACT The Sec61 translocon mediates the translocation of numerous, newly synthesized precursor proteins into the lumen of the endoplasmic reticulum or their integration into its membrane. Recently, structural biology revealed conformations of idle or substrate‐engaged Sec61, and likewise its interactions with the accessory membrane proteins Sec62 ...
Nidhi Sorout, Volkhard Helms
wiley +1 more source
β-lactamase (Bla) Reporter-based System to Study Flagellar Type 3 Secretion in Salmonella
Bio-Protocol, 2023 Export of type 3 secretion (T3S) substrates is traditionally evaluated using trichloroacetic acid (TCA) precipitation of cultured cell supernatants followed by western blot analysis of the secreted substrates.
Fabienne Chevance, Kelly Hughes
doaj +1 more source
Role of a Conserved Glutamate Residue in the \u3cem\u3eEscherichia coli\u3c/em\u3e SecA ATPase Mechanism [PDF]
, 2005 Escherichia coli SecA uses ATP to drive the transport of proteins across cell membranes. Glutamate 210 in the “DEVD” Walker B motif of the SecA ATP-binding site has been proposed as the catalytic base for ATP hydrolysis (Hunt, J. F., Weinkauf, S., Henry,
Antony, Edwin +4 more
core +1 more source