Results 151 to 160 of about 14,594 (219)

Three-step evolutionary enhanced capillary electrophoresis-SELEX for aptamer selection of exosome vesicles.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2023
As various disease biomarkers, drugs/biomolecules carriers and physiological function regulator, exosomes play significant roles in various physiological and pathological process.
Liping Zhao, Ge Yang, Chao Zhu, Linsen Li, Yi Zhao, Yunxia Luan, Feng Qu   +6 more
semanticscholar   +1 more source

Non-immobilized GO-SELEX of aptamers for label-free detection of thiamethoxam in vegetables.

Analytica Chimica Acta, 2022
Due to the massive use of thiamethoxam (TMX) pesticide and the accumulated potential hazards exposure, the detection of TMX is of great significance to food and ecological safety.
Qianqian Kong, Fengli Yue, Mengyue Liu, Jingcheng Huang, Fengzhen Yang, Junjie Liu, Jiansen Li, Falan Li, Xia Sun, Yemin Guo, Yelong Zhu   +10 more
semanticscholar   +1 more source

In silico SELEX screening and statistical analysis of newly designed 5mer peptide-aptamers as Bcl-xl inhibitors using the Taguchi method

Comput. Biol. Medicine, 2022
Drug development for cancer treatment is a complex process that requires special efforts. Targeting crucial proteins is the most essential purpose of drug design in cancers.
Alireza Poustforoosh, Sanaz Faramarz, M. Nematollahi, H. Hashemipour, M. Negahdaripour, A. Pardakhty   +5 more
semanticscholar   +1 more source

SELEX with modified nucleotides

Current Opinion in Chemical Biology, 2008
Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucleotide libraries containing modified nucleotides are ...
Anthony D, Keefe, Sharon T, Cload
openaire   +2 more sources

Non-SELEX Selection of Aptamers

Journal of the American Chemical Society, 2006
Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification. Here we report, for the first time, non-SELEX selection of aptamers-a process that involves repetitive steps of partitioning with no amplification between them.
Maxim V Berezovski, Michael U Musheev, Andrei Drabovich   +2 more
exaly   +3 more sources

GO-SELEX-enhanced dual-recognition sensor for highly specific detection of azamethiphos.

Journal of Hazardous Materials
Organophosphorus pesticides (OPs) have attracted attention due to their widespread application in agriculture and public health sector. Among them, azamethiphos (AZA) may pose risks to non-target organisms and human health through food chain accumulation.
Lingjun Geng, Jingcheng Huang, H. Dong, Haifang Wang, Rui Xu, Zhiping Yu, Ibrahim A. Darwish, Yemin Guo, Xia Sun   +8 more
semanticscholar   +1 more source

Bio-Layer Interferometry-Based SELEX and Label-Free Detection of Patulin Using Generated Aptamer.

Journal of Agricultural and Food Chemistry, 2022
This study reports a novel bio-layer interferometry (BLI)-based SELEX for generation of high affinity aptamers against patulin. Unlike conventional SELEX, the present method enabled real-time monitoring of increasing affinity of the oligonucleotides to ...
M. Mukherjee, Prakruthi Appaiah, S. Sistla, Bettadaiah Bk, P. Bhatt   +4 more
semanticscholar   +1 more source

Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)

2009
SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers.
Renee K, Mosing, Michael T, Bowser
openaire   +2 more sources

Introduction of Aptamer, SELEX, and Different SELEX Variants

2021
In recent years, molecular diagnostic and therapeutic techniques in the field of medical science have been developed rapidly. It is necessary and significant to generate molecular recognition elements with high sensitivity and specificity thereof. Aptamer is an important class of molecular recognition elements, which can be used for disease diagnosis ...
Liling Hao, Huajie Gu
openaire   +1 more source

From cell-SELEX to tissue-SELEX for targeted drug delivery and aptamer nanomedicine.

Advanced Drug Delivery Reviews
Aptamers are nucleic acid-based ligands that can selectively bind to target molecules. Because of their unique target-binding properties, the use of aptamers for targeting cell surface molecules has attracted broad research interest.
Ka-Ying Wong, M. Wong, Jung Heon Lee, Juewen Liu   +3 more
semanticscholar   +1 more source