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Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers
Analytical Chemistry, 2023 Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the enrichment factors of three library-immobilization SELEX methods (capture-SELEX, GO-SELEX, and gold-SELEX).
Yuzhe Ding, Juewen Liu
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Analytical and Bioanalytical Chemistry, 2006
SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification.
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SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification.
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, 2013 SELEX is a robust technique for identifying nucleic acid aptamers with selectable characteristics. SELEX is a combinatorially tedious exhaustive search system, typically involving up to 1010 possible aptamers. Fully understanding functional relationship(s) between sequence and binding for each SELEX round would be prohibited by the sheer quantity of
Tanner, JA, Kinghorn, AB
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CELL-SELEX: Novel Perspectives of Aptamer-Based Therapeutics [PDF]
International Journal of Molecular Sciences, 2008 Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications.
Gerhard Ziemer +2 more
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Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine
International Journal of Molecular Sciences, 2012 Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential ...
Mao Ye, Minyuan Peng, Xielan Zhao
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Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment
Biotechnology Advances, 2022Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized libraries, presenting high affinity and specificity toward targets with similar to antibodies. Recently, aptamers have demonstrated their outstanding application potential ranging from biosensors, bioimaging, and therapeutics due to various advantages including low ...
Shuo, Qi +6 more
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SELEX with modified nucleotides
Current Opinion in Chemical Biology, 2008Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucleotide libraries containing modified nucleotides are ...
Anthony D, Keefe, Sharon T, Cload
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Aptamers: The “evolution” of SELEX
Methods, 2016It has been more than two decades since the first aptamer molecule was discovered. Since then, aptamer molecules have gain much attention in the scientific field. This increasing traction can be attributed to their many desirable traits, such as 1) their potentials to bind a wide range of molecules, 2) their malleability, and 3) their low cost of ...
Yi Xi, Wu, Young Jik, Kwon
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SELEX and the evolution of genomes
Current Opinion in Genetics & Development, 1997The interrupted genome structures of complex multicellular organisms have most likely changed the evolution of the regulation of metabolism and development. Wasted intron sequences make regulation of gene expression in (for example) mammals appear to be unnecessarily complicated. The recent discoveries that globular RNA molecules are very much like the
L, Gold, B, Singer, Y Y, He, E, Brody
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Introduction of Aptamer, SELEX, and Different SELEX Variants
2021In recent years, molecular diagnostic and therapeutic techniques in the field of medical science have been developed rapidly. It is necessary and significant to generate molecular recognition elements with high sensitivity and specificity thereof. Aptamer is an important class of molecular recognition elements, which can be used for disease diagnosis ...
Liling Hao, Huajie Gu
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