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Bio-Layer Interferometry-Based SELEX and Label-Free Detection of Patulin Using Generated Aptamer.
Journal of Agricultural and Food Chemistry, 2022This study reports a novel bio-layer interferometry (BLI)-based SELEX for generation of high affinity aptamers against patulin. Unlike conventional SELEX, the present method enabled real-time monitoring of increasing affinity of the oligonucleotides to ...
M. Mukherjee +4 more
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Design and application of microfluidics in aptamer SELEX and Aptasensors
Biotechnology AdvancesAptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors.
Nuo Duan, Shijia Wu, Zhouping Wang
exaly +2 more sources
Functional SELEX and Biomedical Applications of Aptamers: Beyond Molecular Recognition.
Angewandte ChemieAptamers, developed through SELEX (systematic evolution of ligands by exponential enrichment), are generally short oligonucleotide molecules with remarkable specificity and binding affinity to diverse biological targets.
Minze He +9 more
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In silico approach for Post-SELEX DNA aptamers: A mini-review.
Journal of Molecular Graphics and Modelling, 2021Aptamers are short oligonucleotides that possess high specificity and affinity against their target. Generated via Systematic Evolution of Ligands by Exponential Enrichment, (SELEX) in vitro, they were screened and enriched.
Nor Azlina Ahmad +3 more
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A Beginners Guide to SELEX and DNA Aptamers.
Analytical BiochemistrySELEX stands for "Systematic Evolution of Ligands by Exponential Enrichment." It is an in vitro, iterative, PCR-based, target-specific selection strategy used to generate single-stranded DNA (ssDNA) aptamers that bind a target of interest.
Cameron Stephens +2 more
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Analytica Chimica Acta
This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX).
Ioana Manea +5 more
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This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX).
Ioana Manea +5 more
semanticscholar +1 more source
Analytica Chimica Acta
BACKGROUND Due to its wide application, procymidone has become one of the pesticides with high detection rates in supervision and sampling. Therefore, it is necessary to establish a rapid and efficient method for the detection of procymidone. However, an
Yemin Guo, Jingcheng Huang, Haowei Dong
exaly +2 more sources
BACKGROUND Due to its wide application, procymidone has become one of the pesticides with high detection rates in supervision and sampling. Therefore, it is necessary to establish a rapid and efficient method for the detection of procymidone. However, an
Yemin Guo, Jingcheng Huang, Haowei Dong
exaly +2 more sources
Journal of Genetic Engineering and Biotechnology
Aptamers are single-stranded oligonucleotide sequences capable of binding to specific ligands with high affinity. In this manner, they are like antibodies but have advantages such as lower manufacturing costs, lower immunogenicity, fewer batch-to-batch ...
D. Chinchilla-Cárdenas +6 more
semanticscholar +1 more source
Aptamers are single-stranded oligonucleotide sequences capable of binding to specific ligands with high affinity. In this manner, they are like antibodies but have advantages such as lower manufacturing costs, lower immunogenicity, fewer batch-to-batch ...
D. Chinchilla-Cárdenas +6 more
semanticscholar +1 more source
Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging.
Analytical Chemistry, 2021Cancer has become one of the most common diseases with high mortality in humans. Early and accurate diagnosis of cancer is of great significance to enhance the survival rate of patients.
Lie Li +7 more
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Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches.
Trends in Biotechnology, 2021Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers' versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic ...
Ricardo Oliveira +5 more
semanticscholar +1 more source