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Development of Cell-SELEX Technology and Its Application in Cancer Diagnosis and Therapy

International Journal of Molecular Sciences, 2016
SELEX (systematic evolution of ligands by exponential enrichment) is a process involving the progressive isolation of high selective ssDNA/RNA from a combinatorial single-stranded oligonucleotide library through repeated rounds of binding, partitioning ...
Yuanyuan Yu, Chao Liang, Aiping Lu
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DNA aptamers selection for Staphylococcus aureus cells by SELEX and Cell-SELEX

Molecular Biology Reports, 2022
Staphylococcus aureus is the most common bacteria found in skin, soft tissues, bone, and bone prostheses infections. The aim of this study was to select DNA aptamers for S. aureus to be applied in the diagnosis of bacteria.We used SELEX (Systematic Evolution of Ligands by EXponencial Enrichment) for peptidoglycan followed by cell-SELEX with S.
Mariane Izabella Abreu de Melo, Pricila da Silva Cunha, Iêda Mendes Ferreira, Antero Silva Ribeiro de Andrade   +3 more
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A microfluidic SELEX prototype

Analytical and Bioanalytical Chemistry, 2005
Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential ...
Glen, Hybarger, Joseph, Bynum, Robert F, Williams, James J, Valdes, James P, Chambers   +4 more
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Complex Target SELEX

Accounts of Chemical Research, 2008
Aptamers are non-naturally occurring structured oligonucleotides that may bind to small molecules, peptides, and proteins. Typically, aptamers are generated by an in vitro selection process referred to as SELEX (systematic evolution of ligands by exponential enrichment).
Steven M, Shamah, Judith M, Healy, Sharon T, Cload   +2 more
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A Computational Study of Alternate SELEX

Bulletin of Mathematical Biology, 2014
Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure for identifying nucleic acid (NA) molecules with affinities for specific target species, such as proteins, peptides, or small organic molecules. Here, we extend the work in Seo et al. (Bull Math Biol 72:1623-1665, 2010) (multiple-target SELEX or positive SELEX) and examine
Seo, Yeon-Jung, Nilsen-Hamilton, Marit, Levine, Howard A.   +2 more
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Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)

2009
SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers.
Renee K, Mosing, Michael T, Bowser
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Non-SELEX Selection of Aptamers

Journal of the American Chemical Society, 2006
Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification. Here we report, for the first time, non-SELEX selection of aptamers-a process that involves repetitive steps of partitioning with no amplification between them.
Maxim, Berezovski, Michael, Musheev, Andrei, Drabovich, Sergey N, Krylov   +3 more
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Post-SELEX optimization of aptamers

Analytical and Bioanalytical Chemistry, 2016
Aptamers are functional single-stranded DNA or RNA oligonucleotides, selected in vitro by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which can fold into stable unique three-dimensional structures that bind their target ligands with high affinity and specificity.
Shunxiang, Gao, Xin, Zheng, Binghua, Jiao, Lianghua, Wang   +3 more
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RNA–RNA SELEX

2014
Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
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Patents on SELEX and Therapeutic Aptamers

Recent Patents on DNA & Gene Sequences, 2008
Aptamers, the oligonucleotides (DNA/RNA) that bind to target molecules with high specificity and affinity, have been a focus of therapeutic research for the last two decades. The magnitude of scientific and commercial interest shown for aptamers is not surprising because aptamers have several advantages over other curative modalities, especially ...
Pooja, Dua, Soyoun, Kim, Dong-Ki, Lee
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