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Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)
2009SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers.
Renee K, Mosing, Michael T, Bowser
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Introduction of Aptamer, SELEX, and Different SELEX Variants
2021In recent years, molecular diagnostic and therapeutic techniques in the field of medical science have been developed rapidly. It is necessary and significant to generate molecular recognition elements with high sensitivity and specificity thereof. Aptamer is an important class of molecular recognition elements, which can be used for disease diagnosis ...
Liling Hao, Huajie Gu
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DNA aptamers selection for Staphylococcus aureus cells by SELEX and Cell-SELEX
Molecular Biology Reports, 2022Staphylococcus aureus is the most common bacteria found in skin, soft tissues, bone, and bone prostheses infections. The aim of this study was to select DNA aptamers for S. aureus to be applied in the diagnosis of bacteria.We used SELEX (Systematic Evolution of Ligands by EXponencial Enrichment) for peptidoglycan followed by cell-SELEX with S.
Mariane Izabella Abreu de Melo +3 more
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A microfluidic SELEX prototype
Analytical and Bioanalytical Chemistry, 2005Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential ...
Glen, Hybarger +4 more
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Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment
Biotechnology Advances, 2022Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized libraries, presenting high affinity and specificity toward targets with similar to antibodies. Recently, aptamers have demonstrated their outstanding application potential ranging from biosensors, bioimaging, and therapeutics due to various advantages including low ...
Shuo, Qi +6 more
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Post-SELEX optimization of aptamers
Analytical and Bioanalytical Chemistry, 2016Aptamers are functional single-stranded DNA or RNA oligonucleotides, selected in vitro by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which can fold into stable unique three-dimensional structures that bind their target ligands with high affinity and specificity.
Shunxiang, Gao +3 more
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SELEX: Just another separation?
The Analyst, 2005The potential for quickly isolating high affinity, highly selective ligands generated much excitement when SELEX was first described. Fifteen years later, SELEX has still not achieved widespread acceptance due to limitations in aptamer affinity, stability and throughput.
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Aptamers: The “evolution” of SELEX
Methods, 2016It has been more than two decades since the first aptamer molecule was discovered. Since then, aptamer molecules have gain much attention in the scientific field. This increasing traction can be attributed to their many desirable traits, such as 1) their potentials to bind a wide range of molecules, 2) their malleability, and 3) their low cost of ...
Yi Xi, Wu, Young Jik, Kwon
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2014
Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
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Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
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A Computational Study of Alternate SELEX
Bulletin of Mathematical Biology, 2014Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure for identifying nucleic acid (NA) molecules with affinities for specific target species, such as proteins, peptides, or small organic molecules. Here, we extend the work in Seo et al. (Bull Math Biol 72:1623-1665, 2010) (multiple-target SELEX or positive SELEX) and examine
Seo, Yeon-Jung +2 more
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