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Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers
Analytical Chemistry, 2023Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the enrichment factors of three library-immobilization SELEX methods (capture-SELEX, GO-SELEX, and gold-SELEX).
Yuzhe Ding, Juewen Liu
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Accounts of Chemical Research, 2008
Aptamers are non-naturally occurring structured oligonucleotides that may bind to small molecules, peptides, and proteins. Typically, aptamers are generated by an in vitro selection process referred to as SELEX (systematic evolution of ligands by exponential enrichment).
Steven M, Shamah +2 more
exaly +3 more sources
Aptamers are non-naturally occurring structured oligonucleotides that may bind to small molecules, peptides, and proteins. Typically, aptamers are generated by an in vitro selection process referred to as SELEX (systematic evolution of ligands by exponential enrichment).
Steven M, Shamah +2 more
exaly +3 more sources
Non-SELEX Selection of Aptamers
Journal of the American Chemical Society, 2006Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification. Here we report, for the first time, non-SELEX selection of aptamers-a process that involves repetitive steps of partitioning with no amplification between them.
Maxim V Berezovski +2 more
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SELEX with modified nucleotides
Current Opinion in Chemical Biology, 2008Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucleotide libraries containing modified nucleotides are ...
Anthony D, Keefe, Sharon T, Cload
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Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)
2009SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers.
Renee K, Mosing, Michael T, Bowser
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Introduction of Aptamer, SELEX, and Different SELEX Variants
2021In recent years, molecular diagnostic and therapeutic techniques in the field of medical science have been developed rapidly. It is necessary and significant to generate molecular recognition elements with high sensitivity and specificity thereof. Aptamer is an important class of molecular recognition elements, which can be used for disease diagnosis ...
Liling Hao, Huajie Gu
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DNA aptamers selection for Staphylococcus aureus cells by SELEX and Cell-SELEX
Molecular Biology Reports, 2022Staphylococcus aureus is the most common bacteria found in skin, soft tissues, bone, and bone prostheses infections. The aim of this study was to select DNA aptamers for S. aureus to be applied in the diagnosis of bacteria.We used SELEX (Systematic Evolution of Ligands by EXponencial Enrichment) for peptidoglycan followed by cell-SELEX with S.
Mariane Izabella Abreu de Melo +3 more
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Post-SELEX optimization of aptamers
Analytical and Bioanalytical Chemistry, 2016Aptamers are functional single-stranded DNA or RNA oligonucleotides, selected in vitro by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which can fold into stable unique three-dimensional structures that bind their target ligands with high affinity and specificity.
Shunxiang Gao, Binghua Jiao
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A microfluidic SELEX prototype
Analytical and Bioanalytical Chemistry, 2005Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential ...
Glen, Hybarger +4 more
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Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment
Biotechnology Advances, 2022Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized libraries, presenting high affinity and specificity toward targets with similar to antibodies. Recently, aptamers have demonstrated their outstanding application potential ranging from biosensors, bioimaging, and therapeutics due to various advantages including low ...
Shuo, Qi +6 more
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