Semi-rational design of an aromatic dioxygenase by substrate tunnel redirection [PDF]
iScienceSummary: Lignin valorization is crucial for achieving economic and sustainable biorefinery processes. However, the enzyme substrate preferences involved in lignin degradation remain poorly understood, and low activity toward specific substrates presents ...
Jiawei Wang +10 more
doaj +3 more sources
Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design [PDF]
AMB Express, 2017 Aldaric acids represent biobased ‘top value-added chemicals’ that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents.
Teresa Roth +3 more
doaj +6 more sources
Enhancing the Catalytic Activity of Glycolate Oxidase from Chlamydomonas reinhardtii through Semi-Rational Design [PDF]
Microorganisms, 2023 Glycolate oxidase is a peroxisomal flavoprotein catalyzing the oxidation of glycolate to glyoxylate and plays crucial metabolic roles in green algae, plants, and animals.
Yingting Feng +9 more
doaj +4 more sources
Advances in semi-rational design of terpene synthases and their modifying enzymes [PDF]
AMB ExpressAs research on natural terpene compounds advances, their pharmacological properties and commercial potential are increasingly recognized. Microbial cell factories offer an efficient means of producing high-value terpene natural products through ...
Xiqin Liang +8 more
doaj +3 more sources
Semi-Rational Design of L-Isoleucine Dioxygenase Generated Its Activity for Aromatic Amino Acid Hydroxylation [PDF]
Molecules, 2023 Fe (II)-and 2-ketoglutarate-dependent dioxygenases (Fe (II)/α-KG DOs) have been applied to catalyze hydroxylation of amino acids. However, the Fe (II)/α-KG DOs that have been developed and characterized are not sufficient.
Jianhong An, Jiaojiao Guan, Yao Nie
doaj +4 more sources
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1 [PDF]
Synthetic and Systems BiotechnologyHydroxysalidroside is an important natural phenylethanoid glycoside with broad application prospects in the food and pharmaceutical fields. However, its low concentration in plants and complex extraction hinder its production.
Xinru Wang +8 more
doaj +4 more sources
Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol in Saccharomyces cerevisiae [PDF]
Synthetic and Systems BiotechnologyTyrosol is an important component of pharmaceuticals, nutraceuticals, and cosmetics, and their biosynthetic pathways are currently a hot research topic.
Na Song +13 more
doaj +4 more sources
Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 As a natural sweetener and sucrose substitute, the biosynthesis and application of steviol glycosides containing the component rebaudioside D have attracted worldwide attention.
Meiqi Chen +6 more
doaj +2 more sources
Enhancing Caffeic Acid Production in Escherichia coli Through Heterologous Enzyme Combinations and Semi-Rational Design [PDF]
MetabolitesBackground/Objectives: Caffeic acid is a hydroxycinnamic acid that has a wide range of applications in the medical field. The synthesis of caffeic acid using microbial fermentation technology is an environmentally friendly method.
Qing Luo +7 more
doaj +2 more sources
Engineering of Laccase CueO for Improved Electron Transfer in Bioelectrocatalysis by Semi-Rational Design. [PDF]
Chemistry, 2020 AbstractCopper efflux oxidase (CueO) from Escherichia coli is a special bacterial laccase due to its fifth copper binding site. Herein, it is discovered that the fifth Cu occupancy plays a crucial and favorable role of electron relay in bioelectrocatalytic oxygen reduction. By substituting the residues at the four coordinated positions of the fifth Cu,
Zhang L +6 more
europepmc +6 more sources