Semi-rational design and molecular dynamics simulations study of the thermostability enhancement of cellobiose 2-epimerases. [PDF]
Int J Biol Macromol, 2020 Directed evolution using random mutation in vast sequence space leads to the low probability of obtaining target proteins. Emerging engineering strategies with computational tools are developed for more trustable outcomes. We used some semi-rational design methods to modify an industrial enzyme, namely cellobiose 2-epimerase (CE). A mutant was selected
Chen Q +4 more
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Structure-guided semi-rational design of an imine reductase for enantio-complementary synthesis of pyrrolidinamine. [PDF]
Chem Sci, 2023 In this study, engineered imine reductases (IREDs) of IRED M5, originally fromActinoalloteichus hymeniacidonis, were obtained through structure-guided semi-rational design.
Zhang J +6 more
europepmc +3 more sources
Semi-rational design of nitroarene dioxygenase for catalytic ability toward 2,4-dichloronitrobenzene. [PDF]
Appl Environ MicrobiolABSTRACT Rieske non-heme dioxygenase family enzymes play an important role in the aerobic biodegradation of nitroaromatic pollutants, but no active dioxygenases are available in nature for initial reactions in the degradation of many refractory pollutants like 2,4-dichloronitrobenzene (24DCNB).
Xu J, Li T, Huang WE, Zhou N-Y.
europepmc +3 more sources
Rational Design of Topical Semi-Solid Dosage Forms-How Far Are We?
Pharmaceutics, 2023 Specific aspects of semi-solid dosage forms for topical application include the nature of the barrier to be overcome, aspects of susceptibility to physical and chemical instability, and a greater influence of sensory perception. Advances in understanding
Michael E. Herbig +3 more
doaj +3 more sources
Enhancing the imidase activity of BpIH toward 3-isobutyl glutarimide via semi-rational design. [PDF]
Appl Microbiol Biotechnol(R)-3-Isobutylglutarate monoamide (R-IBM) is a key intermediate in the synthesis of the analgesic drug pregabalin. Recently, the imidase BpIH derived from Burkholderia phytofirmans was identified as a promising catalyst for the industrial production of R-IBM. Notably, this catalyst has the distinct advantage of achieving a 100% theoretical yield from 3-
Qin W, Xu L, Cheng K, Lu Y, Yang Z.
europepmc +3 more sources
Enhancing the Catalytic Activity of Type II L-Asparaginase from Bacillus licheniformis through Semi-Rational Design. [PDF]
Int J Mol Sci, 2022 Low catalytic activity is a key factor limiting the widespread application of type II L-asparaginase (ASNase) in the food and pharmaceutical industries. In this study, smart libraries were constructed by semi-rational design to improve the catalytic activity of type II ASNase from Bacillus licheniformis.
Zhou Y +7 more
europepmc +3 more sources
Development of thermostable sucrose phosphorylase by semi-rational design for efficient biosynthesis of alpha-D-glucosylglycerol. [PDF]
Appl Microbiol Biotechnol, 2021 Abstract Sucrose phosphorylase (SPase) can specifically catalyze transglycosylation reactions and can be used to enzymatically synthesize α-D-glycosides. However, the low thermostability of SPase has been a bottleneck for its industrial application. In this study, a SPase gene from Leuconostoc mesenteroides ATCC 12,291 (
Xia Y +7 more
europepmc +4 more sources
Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase [PDF]
AMB Express, 2017 Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production.
Kyung-Chul Shin +3 more
doaj +2 more sources
Enhanced catalytic activity of a novel trypsin by semi-rational design with mechanistic insights from molecular simulations [PDF]
Food Chemistry: Molecular SciencesTrypsin is widely used in the food industry for meat processing, dairy production and seafood treatment. However, the industrial application of trypsin is constrained by the pathogenic risks associated with animal-derived trypsin and the low enzymatic ...
Jia Chen +2 more
doaj +2 more sources
Beyond directed evolution—semi-rational protein engineering and design [PDF]
Current Opinion in Biotechnology, 2010 Over the past two decades, directed evolution has transformed the field of protein engineering. The advances in understanding protein structure and function, in no insignificant part a result of directed evolution studies, are increasingly empowering scientists and engineers to device more effective methods for manipulating and tailoring biocatalysts ...
Stefan Lutz
openaire +4 more sources