Results 91 to 100 of about 3,720,026 (349)

Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination [PDF]

, 2011
Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3-5-kb DNA fragment are not easy to identify and pose ...
Deforce, Dieter, Gaasterland, Terry, Head, Steven R, Ledesma, Jessica, Ordoukhanian, Phillip, Thompson, Ryan C, Van Nieuwerburgh, Filip   +6 more
core   +2 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Genome sequencing data for wild and cultivated bananas, plantains and abacá

Data in Brief, 2020
We performed shotgun genome sequencing on a total of 19 different Musa genotypes including representatives of wild banana species Musa acuminata and M. balibisiana, allopolyploid bananas and plantains, Fe'i banana, pink banana (also known as hairy banana)
Christine Sambles, Lakshmipriya Venkatesan, Olanrewaju M. Shittu, James Harrison, Karen Moore, Leena Tripathi, Murray Grant, Rachel Warmington, David J. Studholme   +8 more
doaj   +1 more source

DeepSNVMiner: a sequence analysis tool to detect emergent, rare mutations in subsets of cell populations [PDF]

PeerJ, 2016
Background. Massively parallel sequencing technology is being used to sequence highly diverse populations of DNA such as that derived from heterogeneous cell mixtures containing both wild-type and disease-related states.
T. Daniel Andrews, Yogesh Jeelall, Dipti Talaulikar, Christopher C. Goodnow, Matthew A. Field   +4 more
doaj   +2 more sources

EFFICIENT PROPAGATION OF ARCHETYPE JC POLYOMAVIRUS IN COS-7 CELLS: EVALUATION OF REARRANGEMENTS WITHIN NCCR STRUCTURAL ORGANIZATION DURING TRANSFECTION. [PDF]

, 2017
John Cunningham virus (JCPyV) is an ubiqui-tous human pathogen that causes disease in immunocom-promised patients. The JCPyV genome is composed of an early region and a late region, which are physically sepa-rated by the ...
Anzivino, Elena, Bellizzi, Anna, Palamara, ANNA TERESA, Pietropaolo, Valeria Antonietta, Prezioso, Carla, Rodio, DONATELLA MARIA, Scribano, D, Trancassini, Maria   +7 more
core   +1 more source

Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice

FEBS Letters, EarlyView.
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász, Albert Bálint Papp, Lukács Sándor Lesinszki, Attila Mócsai, Tamás Varga, László Nagy, Péter Gogolák   +6 more
wiley   +1 more source

Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali. [PDF]

PLoS ONE, 2011
Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis.
Takeshi Terahara, Seinen Chow, Hiroaki Kurogi, Sun-Hee Lee, Katsumi Tsukamoto, Noritaka Mochioka, Hideki Tanaka, Haruko Takeyama   +7 more
doaj   +1 more source

Mechanistic studies on DNA damage by minor groove binding copper–phenanthroline conjugates [PDF]

, 2005
Copper–phenanthroline complexes oxidatively damage and cleave nucleic acids. Copper bis-phenanthroline and copper complexes of mono- and bis-phenanthroline conjugates are used as research tools for studying nucleic acid structure and binding interactions.
Bales, Brian C., Greenberg, Marc M., Kodama, Tetsuya, Meunier, Bernard, Pitié, Marguerite, Weledji, Yvonne N.   +5 more
core   +2 more sources

Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution

FEBS Letters, EarlyView.
The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai, Richard J. McDowell, Robert J. Lucas   +2 more
wiley   +1 more source

Structure of 5S rDNA sequence of the gibel carp (Carassius gibelio) with 2n=100 chromosomes

Frontiers in Marine Science, 2015
In vertebrate, the 5S ribosomal DNA (rDNA) consists of a conserved coding (transcriptional) unit of 120 bp (5S), which is separated from the next unit by a nontranscribed spacer (NTS). The coding sequence and the NTS together form a repeat unit which can
Lech Kirtiklis
doaj   +1 more source