Results 31 to 40 of about 1,180,805 (214)

The SH2 Domain Interaction Landscape

Cell Reports, 2013
Members of the SH2 domain family modulate signal transduction by binding to short peptides containing phosphorylated tyrosines. Each domain displays a distinct preference for the sequence context of the phosphorylated residue.
Michele Tinti, Lars Kiemer, Stefano Costa, Martin L. Miller, Francesca Sacco, Jesper V. Olsen, Martina Carducci, Serena Paoluzi, Francesca Langone, Christopher T. Workman, Nikolaj Blom, Kazuya Machida, Christopher M. Thompson, Mike Schutkowski, Søren Brunak, Matthias Mann, Bruce J. Mayer, Luisa Castagnoli, Gianni Cesareni   +18 more
doaj   +1 more source

Chronic AMPK inactivation slows SHH medulloblastoma progression by inhibiting mTORC1 signaling and depleting tumor stem cells

iScience, 2023
Summary: We show that inactivating AMPK in a genetic medulloblastoma model depletes tumor stem cells and slows progression. In medulloblastoma, the most common malignant pediatric brain tumor, drug-resistant stem cells co-exist with transit-amplifying ...
Daniel Shiloh Malawsky, Taylor Dismuke, Hedi Liu, Ethan Castellino, Jay Brenman, Biplab Dasgupta, Andrey Tikunov, Timothy R. Gershon   +7 more
doaj   +1 more source

Sequencing Enabling Design and Learning in Synthetic Biology

Current Opinion in Chemical Biology, 2020
The ability to read and quantify nucleic acids such as DNA and RNA using sequencing technologies has revolutionized our understanding of life. With the emergence of synthetic biology, these tools are now being put to work in new ways - enabling de novo biological design.
Gilliot, Pierre-Aurelien M A, Gorochowski, Thomas E   +1 more
openaire   +4 more sources

Biology of Genomes: making sense of sequence [PDF]

Genome Medicine, 2009
A report on the Biology of Genomes meeting held at Cold Spring Harbor Laboratory, NY, USA, 5-9 May 2009.
openaire   +2 more sources

Natural variability of Kozak sequences correlates with function in a zebrafish model. [PDF]

PLoS ONE, 2014
In eukaryotes, targeting the small ribosomal subunit to the mRNA transcript requires a Kozak sequence at the translation initiation site. Despite the critical importance of the Kozak sequence to regulation of gene expression, there have been no ...
Steven J Grzegorski, Estelle F Chiari, Amy Robbins, Phillip E Kish, Alon Kahana   +4 more
doaj   +1 more source

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

STAR Protocols, 2022
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin.
Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo   +3 more
doaj   +1 more source

Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

Viruses, 2016
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease.
Jens Friis-Nielsen, Kristín Rós Kjartansdóttir, Sarah Mollerup, Maria Asplund, Tobias Mourier, Randi Holm Jensen, Thomas Arn Hansen, Alba Rey-Iglesia, Stine Raith Richter, Ida Broman Nielsen, David E. Alquezar-Planas, Pernille V. S. Olsen, Lasse Vinner, Helena Fridholm, Lars Peter Nielsen, Eske Willerslev, Thomas Sicheritz-Pontén, Ole Lund, Anders Johannes Hansen, Jose M. G. Izarzugaza, Søren Brunak   +20 more
doaj   +1 more source

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

STAR Protocols, 2023
Summary: Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences.
Yanyan Wang, Senxin Zhang, Xiaoqi Zheng, Leng-Siew Yeap, Fei-Long Meng   +4 more
doaj   +1 more source

Single-cell sequencing in stem cell biology

Genome Biology, 2016
Cell-to-cell variation and heterogeneity are fundamental and intrinsic characteristics of stem cell populations, but these differences are masked when bulk cells are used for omic analysis. Single-cell sequencing technologies serve as powerful tools to dissect cellular heterogeneity comprehensively and to identify distinct phenotypic cell types, even ...
Wen, Lu, Tang, Fuchou
openaire   +2 more sources

FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

Journal of Immunology Research, 2015
FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity ...
Christian Simon, Ulrich J. Kudahl, Jing Sun, Lars Rønn Olsen, Guang Lan Zhang, Ellis L. Reinherz, Vladimir Brusic   +6 more
doaj   +1 more source