Results 41 to 50 of about 2,275,975 (294)

Natural variability of Kozak sequences correlates with function in a zebrafish model. [PDF]

PLoS ONE, 2014
In eukaryotes, targeting the small ribosomal subunit to the mRNA transcript requires a Kozak sequence at the translation initiation site. Despite the critical importance of the Kozak sequence to regulation of gene expression, there have been no ...
Steven J Grzegorski, Estelle F Chiari, Amy Robbins, Phillip E Kish, Alon Kahana   +4 more
doaj   +1 more source

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

STAR Protocols, 2022
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin.
Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo   +3 more
doaj   +1 more source

BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis [PDF]

, 2004
Plant steroid hormones, brassinosteroids (BRs), are perceived by the plasma membrane-localized leucine-rich-repeat-receptor kinase BRI1. Based on sequence similarity, we have identified three members of the BRI1 family, named BRL1, BRL2 and BRL3.
Caño Delgado, Ana, Cheng, Jin Chen, Chory, Joanne, Li, Jianming, Mora Garcia, Santiago, Nam, Kyoung Hee, Vafeados, Dionne, Yin, Yanhai, Yu, Cong   +8 more
core   +1 more source

Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

Viruses, 2016
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease.
Jens Friis-Nielsen, Kristín Rós Kjartansdóttir, Sarah Mollerup, Maria Asplund, Tobias Mourier, Randi Holm Jensen, Thomas Arn Hansen, Alba Rey-Iglesia, Stine Raith Richter, Ida Broman Nielsen, David E. Alquezar-Planas, Pernille V. S. Olsen, Lasse Vinner, Helena Fridholm, Lars Peter Nielsen, Eske Willerslev, Thomas Sicheritz-Pontén, Ole Lund, Anders Johannes Hansen, Jose M. G. Izarzugaza, Søren Brunak   +20 more
doaj   +1 more source

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

STAR Protocols, 2023
Summary: Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences.
Yanyan Wang, Senxin Zhang, Xiaoqi Zheng, Leng-Siew Yeap, Fei-Long Meng   +4 more
doaj   +1 more source

More Mouldy Data: Another mycoplasma gene jumps the silicon barrier into the human genome

, 2011
The human genome sequence database contains DNA sequences very like those of mycoplasma molds. It appears such moulds infect not only molecular Biology laboratories but were picked up by experimenters from contaminated samples and inserted into GenBank ...
Arno, M. J., Langdon, W. B.
core   +1 more source

Techniques for RNA in vivo imaging in plants [PDF]

, 2014
Since the discovery of small RNAs and RNA silencing, RNA biology has taken a centre stage in cell and developmental biology. Small RNAs, but also mRNAs and other types of cellular and viral RNAs are processed at specific subcellular localizations.
Tilsner, Jens
core   +1 more source

Applications of next‐generation sequencing in plant biology [PDF]

American Journal of Botany, 2012
The last several years have seen revolutionary advances in DNA sequencing technologies with the advent of next‐generation sequencing (NGS) techniques. NGS methods now allow millions of bases to be sequenced in one round, at a fraction of the cost relative to traditional Sanger sequencing.
Ashley N, Egan, Jessica, Schlueter, David M, Spooner   +2 more
openaire   +2 more sources

FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

Journal of Immunology Research, 2015
FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity ...
Christian Simon, Ulrich J. Kudahl, Jing Sun, Lars Rønn Olsen, Guang Lan Zhang, Ellis L. Reinherz, Vladimir Brusic   +6 more
doaj   +1 more source

Dynamics of Unperturbed and Noisy Generalized Boolean Networks

, 2009
For years, we have been building models of gene regulatory networks, where recent advances in molecular biology shed some light on new structural and dynamical properties of such highly complex systems.
Darabos, Christian, Giacobini, Mario, Tomassini, Marco   +2 more
core   +1 more source