Results 41 to 50 of about 1,274,222 (313)

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

STAR Protocols, 2022
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin.
Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo   +3 more
doaj   +1 more source

Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

Viruses, 2016
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease.
Jens Friis-Nielsen, Kristín Rós Kjartansdóttir, Sarah Mollerup, Maria Asplund, Tobias Mourier, Randi Holm Jensen, Thomas Arn Hansen, Alba Rey-Iglesia, Stine Raith Richter, Ida Broman Nielsen, David E. Alquezar-Planas, Pernille V. S. Olsen, Lasse Vinner, Helena Fridholm, Lars Peter Nielsen, Eske Willerslev, Thomas Sicheritz-Pontén, Ole Lund, Anders Johannes Hansen, Jose M. G. Izarzugaza, Søren Brunak   +20 more
doaj   +1 more source

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

STAR Protocols, 2023
Summary: Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences.
Yanyan Wang, Senxin Zhang, Xiaoqi Zheng, Leng-Siew Yeap, Fei-Long Meng   +4 more
doaj   +1 more source

Sequence Alignment in Molecular Biology

, 1998
Molecular biology is becoming a computationally intense realm of contemporary science and faces some of the current grand scientific challenges. In its context, tools that identify, store, compare and analyze effectively large and growing numbers of bio ...
APOSTOLICO, ALBERTO, R. GIANCARLO
core   +1 more source

Genetic Relationships among Reptilian and Mammalian Campylobacter fetus Strains Determined by Multilocus Sequence Typing [PDF]

, 2010
Reptile Campylobacter fetus isolates and closely related strains causing human disease were characterized by multilocus sequence typing. They shared similar to 90% nucleotide sequence identity with classical mammalian C.
Pruckler, J., Dep Infectieziekten Immunologie, Fitzgerald, C., van Bergen, M.A.P., Marcel A. P. van Bergen, Bergen, M.A.P., van, Robert J. Owen, Tu, Z.C., Kate E. Dingle, Janet Pruckler, Blaser, M.J., van Bergen, M.A., Strategic Infection Biology, Dingle, K.E., Zheng-Chao Tu, Collette Fitzgerald, Lawson, A.J., Wagenaar, J.A., Martin J. Blaser, Andrew J. Lawson, Jaap A. Wagenaar, Owen, R.J.   +21 more
core   +1 more source

Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.

Journal of Molecular Biology, 1999
An improved dynamic programming algorithm is reported for RNA secondary structure prediction by free energy minimization. Thermodynamic parameters for the stabilities of secondary structure motifs are revised to include expanded sequence dependence as ...
D. Mathews, Jeffrey Sabina, M. Zuker, D. Turner   +3 more
semanticscholar   +1 more source

FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

Journal of Immunology Research, 2015
FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity ...
Christian Simon, Ulrich J. Kudahl, Jing Sun, Lars Rønn Olsen, Guang Lan Zhang, Ellis L. Reinherz, Vladimir Brusic   +6 more
doaj   +1 more source

An iterative method for faster sum-of-pairs multiple sequence alignment

, 2000
Reinert K, Stoye J, Will T. An iterative method for faster sum-of-pairs multiple sequence alignment. Bioinformatics.
Stoye, Jens ; https://orcid.org/, Reinert, Knut, Will, Torsten   +2 more
core   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Development of a Biocontained Toluene-Degrading Bacterium for Environmental Protection

Microbiology Spectrum, 2021
Biocontainment is a safeguard strategy for preventing uncontrolled proliferation of genetically engineered microorganisms (GEMs) in the environment. Biocontained GEMs are designed to survive only in the presence of a specific molecule.
Masahito Ishikawa, Takaaki Kojima, Katsutoshi Hori   +2 more
doaj   +1 more source