Results 41 to 50 of about 2,264,396 (196)

Natural variability of Kozak sequences correlates with function in a zebrafish model. [PDF]

PLoS ONE, 2014
In eukaryotes, targeting the small ribosomal subunit to the mRNA transcript requires a Kozak sequence at the translation initiation site. Despite the critical importance of the Kozak sequence to regulation of gene expression, there have been no ...
Steven J Grzegorski, Estelle F Chiari, Amy Robbins, Phillip E Kish, Alon Kahana   +4 more
doaj   +1 more source

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

STAR Protocols, 2022
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin.
Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo   +3 more
doaj   +1 more source

Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

Viruses, 2016
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease.
Jens Friis-Nielsen, Kristín Rós Kjartansdóttir, Sarah Mollerup, Maria Asplund, Tobias Mourier, Randi Holm Jensen, Thomas Arn Hansen, Alba Rey-Iglesia, Stine Raith Richter, Ida Broman Nielsen, David E. Alquezar-Planas, Pernille V. S. Olsen, Lasse Vinner, Helena Fridholm, Lars Peter Nielsen, Eske Willerslev, Thomas Sicheritz-Pontén, Ole Lund, Anders Johannes Hansen, Jose M. G. Izarzugaza, Søren Brunak   +20 more
doaj   +1 more source

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

STAR Protocols, 2023
Summary: Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences.
Yanyan Wang, Senxin Zhang, Xiaoqi Zheng, Leng-Siew Yeap, Fei-Long Meng   +4 more
doaj   +1 more source

An improved Plasmodium cynomolgi genome assembly reveals an unexpected methyltransferase gene expansion. [PDF]

, 2017
Background: Plasmodium cynomolgi, a non-human primate malaria parasite species, has been an important model parasite since its discovery in 1907. Similarities in the biology of P.
Berriman, Matt, Böhme, Ulrike, Kocken, Clemens H.M., Otto, Thomas Dan, Pasini, Erica M., Rutledge, Gavin G., Sanders, Mandy, Voorberg-Van der Wel, Annemarie   +7 more
core   +2 more sources

Single-cell sequencing in stem cell biology

Genome Biology, 2016
Cell-to-cell variation and heterogeneity are fundamental and intrinsic characteristics of stem cell populations, but these differences are masked when bulk cells are used for omic analysis. Single-cell sequencing technologies serve as powerful tools to dissect cellular heterogeneity comprehensively and to identify distinct phenotypic cell types, even ...
Wen, Lu, Tang, Fuchou
openaire   +2 more sources

FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

Journal of Immunology Research, 2015
FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity ...
Christian Simon, Ulrich J. Kudahl, Jing Sun, Lars Rønn Olsen, Guang Lan Zhang, Ellis L. Reinherz, Vladimir Brusic   +6 more
doaj   +1 more source

Techniques for RNA in vivo imaging in plants [PDF]

, 2014
Since the discovery of small RNAs and RNA silencing, RNA biology has taken a centre stage in cell and developmental biology. Small RNAs, but also mRNAs and other types of cellular and viral RNAs are processed at specific subcellular localizations.
Tilsner, Jens
core   +1 more source

Decoding Sequence Classification Models for Acquiring New Biological Insights [PDF]

, 2010
Classifying biological sequences is one of the most important tasks in computational biology. In the last decade, support vector machines (SVMs) in combination with sequence kernels have emerged as a de-facto standard.
Andreas Kothmeier, Carsten Mahrenholz, Ingrid Abfalter, Sepp Hochreiter, Ulrich Bodenhofer, Ulrich Bodenhofer   +5 more
core   +2 more sources

Building Block and Building Rule: Dual Descriptor Method for Biological Sequence Analysis [PDF]

, 2008
The emergence of “Systems Biology” in recent years highlights the systematic viewpoint of bio-system modeling. Building on such a background, Dual Descriptor Method, a generic methodology for biological sequence analysis is proposed. From a
Bin-Guang Ma
core   +1 more source