Results 51 to 60 of about 5,890,604 (383)

Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation

open access: yesiScience, 2023
Summary: Copper is an essential component in the mitochondrial respiratory chain complex IV (cytochrome c oxidases). However, whether any nucleolar factor(s) is(are) involved in regulating the mitochondrial copper homeostasis remains unclear.
Jinsong Wei   +9 more
doaj   +1 more source

Biopython: freely available Python tools for computational molecular biology and bioinformatics

open access: yesBioinform., 2009
Summary: The Biopython project is a mature open source international collaboration of volunteer developers, providing Python libraries for a wide range of bioinformatics problems. Biopython includes modules for reading and writing different sequence file
P. Cock   +10 more
semanticscholar   +1 more source

The Technology and Biology of Single-Cell RNA Sequencing [PDF]

open access: yesMolecular Cell, 2015
The differences between individual cells can have profound functional consequences, in both unicellular and multicellular organisms. Recently developed single-cell mRNA-sequencing methods enable unbiased, high-throughput, and high-resolution transcriptomic analysis of individual cells. This provides an additional dimension to transcriptomic information
Kolodziejczyk, Aleksandra A.   +4 more
openaire   +2 more sources

Natural variability of Kozak sequences correlates with function in a zebrafish model. [PDF]

open access: yesPLoS ONE, 2014
In eukaryotes, targeting the small ribosomal subunit to the mRNA transcript requires a Kozak sequence at the translation initiation site. Despite the critical importance of the Kozak sequence to regulation of gene expression, there have been no ...
Steven J Grzegorski   +4 more
doaj   +1 more source

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

open access: yesSTAR Protocols, 2022
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin.
Ratklao Siriwach   +3 more
doaj   +1 more source

Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

open access: yesViruses, 2016
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease.
Jens Friis-Nielsen   +20 more
doaj   +1 more source

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences

open access: yesSTAR Protocols, 2023
Summary: Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences.
Yanyan Wang   +4 more
doaj   +1 more source

BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis [PDF]

open access: yes, 2004
Plant steroid hormones, brassinosteroids (BRs), are perceived by the plasma membrane-localized leucine-rich-repeat-receptor kinase BRI1. Based on sequence similarity, we have identified three members of the BRI1 family, named BRL1, BRL2 and BRL3.
Caño Delgado, Ana   +8 more
core   +1 more source

Applications of next‐generation sequencing in plant biology [PDF]

open access: yesAmerican Journal of Botany, 2012
The last several years have seen revolutionary advances in DNA sequencing technologies with the advent of next‐generation sequencing (NGS) techniques. NGS methods now allow millions of bases to be sequenced in one round, at a fraction of the cost relative to traditional Sanger sequencing.
Ashley N, Egan   +2 more
openaire   +2 more sources

FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

open access: yesJournal of Immunology Research, 2015
FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity ...
Christian Simon   +6 more
doaj   +1 more source

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