Viromic analysis of cerebrospinal fluid (CSF) samples identified an unclassified RNA virus exhibiting an atypical genomic architecture. [PDF]
Zhang J +9 more
europepmc +1 more source
ABSTRACT Pangenome can reveal a large number of variations, providing a more comprehensive view of the genetic diversity of species that a single reference genome cannot surpass. Here, we assembled the haplotype telomere‐to‐telomere genome and 10 chromosome‐level genomes, integrated with two previously reported genomes, and constructed a graph ...
Xiao Huang +15 more
wiley +1 more source
The Emergence of a CRISPR-Cas Revolution in Ecology: Applications, Challenges, and an Ecologist's Overview of the Toolbox. [PDF]
Plewnia A +8 more
europepmc +1 more source
LUCID: An Integrative Approach for Target Discovery and dsRNA Design in Plant Fungal Pathogens
LUCID: A computational pipeline for RNAi‐based biofungicide design. ABSTRACT Phytopathogenic fungi pose an escalating threat to global food security and ecosystem stability, as resistance and environmental concerns diminish the effectiveness of conventional fungicides.
Lucía Jiménez‐Castro +4 more
wiley +1 more source
ABSTRACT Efficient viral proliferation within the host is a critical step in pathogenicity and requires adenosine triphosphate (ATP). The replication, movement and immune evasion of many plant viruses within their hosts are associated with phase separation (PS)‐derived aggregates formed by viral components.
Guangcheng Zu +8 more
wiley +1 more source
Molecular characterization of porcine respiratory and reproductive syndrome virus (PRRSV), a member of the arterivirus group [PDF]
Conzelmann, Karl-Klaus +3 more
core
Evaluation of the pathogenicity of a rescued avian metapneumovirus subtype B strain in China. [PDF]
Yu Z +6 more
europepmc +1 more source
ABSTRACT The synthesis and accumulation of active ingredients in medicinal plants are distributed in specific organs, tissues, and cell types, which are important for the exploitation of medicinal plants. However, the fine distribution of active ingredients is difficult to know. Here, the system of mass spectrometry imaging (MSI) integrated with single‐
HaiSheng Zeng +14 more
wiley +1 more source
Analysis of the Characteristics of Measles Virus D8 Genotype Based on the Nucleoprotein (N-450) Gene Region - Gansu Province, China, 2024. [PDF]
Wei H +7 more
europepmc +1 more source
Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew +7 more
wiley +1 more source

