Results 71 to 80 of about 2,098,916 (269)

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

ParMap, an Algorithm for the Identification of Complex Genomic Variations in Nextgen Sequencing Data

, 2010
Next-generation sequencing produces high-throughput data, albeit with greater error and shorter reads than traditional Sanger sequencing methods. This complicates the detection of genomic variations, especially, small insertions and deletions.
Raul Rabadan, Adolfo A. Ferrando, Teresa Palomero, Hossein Khiabanian, Pieter Van Vlierberghe   +4 more
core  

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples. [PDF]

, 2012
peer-reviewedBackground: The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an ...
Magee, David A, Mullen Michael P, Creevey Christopher J, Park, Stephen D, Killeen Aideen P, Donagh P Berry, Killeen, Aideen P, Park Stephen D, Howard, Dawn J, McGettigan, Paul A., Christopher J Creevey, Creevey, Christopher J.; id_orcid, Matt C Lucy, Michael P Mullen, Waters, Sinead M., Howard Dawn J, David A Magee, Howard, Dawn J., Lucy Matt C, Mullen, Michael P, Berry Donagh P, Berry, Donagh P., McCabe, Matt S., Park, Stephen D., Magee, David A., McCabe Matt S, Dawn J Howard, Creevey, Christopher J., Matt S McCabe, Lucy, Matt C., Waters, Sinead M, McCabe, Matt S, Killeen, Aideen P., Lucy, Matt C, Magee David A, McGettigan Paul A, Paul A McGettigan, Waters Sinead M, MacHugh, David E, MacHugh David E, David E MacHugh, Aideen P Killeen, MacHugh, David E., Park, Stephen D. E., McGettigan, Paul A, McGettigan, Paul, Mullen, Michael P., McCabe, Matthew, Creevey, Christopher J, Berry, Donagh P, Sinead M Waters, Stephen D Park, Berry, Donagh   +52 more
core   +1 more source

The sequence memoizer

Communications of the ACM, 2011
Probabilistic models of sequences play a central role in most machine translation, automated speech recognition, lossless compression, spell-checking, and gene identification applications to name but a few. Unfortunately, real-world sequence data often exhibit long range dependencies which can only be captured by computationally challenging, complex ...
Wood, Frank, Gasthaus, Jan, Archambeau, Cedric, James, Lancelot, Teh, Yee Whye   +4 more
openaire   +2 more sources

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform [PDF]

, 2014
Background Many high throughput sequencing (HTS) approaches, such as the Roche/454 platform, produce sequences in which the quality of the sequence (as measured by a Phred-like quality scores) decreases linearly across a sequence read.
Grace P McCormack, Lubinsky, Baruch, Bansode, Vijay B., Mónica BJ Moinz, McCormack, Grace P., Vijay B Bansode, Travers, Simon A., Simon A Travers, Ram Shrestha, Baruch Lubinsky, Shrestha, Ram, Moinz, Mónica B. J.   +11 more
core   +1 more source

Ubiquitination of secretory granules promotes their crinophagic degradation in Drosophila

FEBS Letters, EarlyView.
Ubiquitination of secretory granules in Drosophila larval salivary glands is a critical molecular trigger for crinophagy, the lysosomal degradation of unreleased, or low‐quality granules. The E3 ubiquitin ligase Cnot4 is recruited to the surface of secretory granules to induce crinophagy.
Tamás Csizmadia, Anna Dósa, Asha Kiran Maddali, András Jipa, Hajnalka Laczkó‐Dobos, Péter Lőw, Gábor Juhász   +6 more
wiley   +1 more source

Bioinformatics' approaches to detect genetic variation in whole genome sequencing data

, 2010
Current genetic marker repositories are not sufficient or even are completely lacking for most farm animals. However, genetic markers are essential for the development of a research tool facilitating discovery of genetic factors that contribute to ...
Kerstens, H.H.D.
core