Results 71 to 80 of about 3,190,493 (303)
BARCOSEL: a tool for selecting an optimal barcode set for high-throughput sequencing
BMC Bioinformatics, 2018 Background Current high-throughput sequencing platforms provide capacity to sequence multiple samples in parallel. Different samples are labeled by attaching a short sample specific nucleotide sequence, barcode, to each DNA molecule prior pooling them ...
Panu Somervuo +5 more
doaj +1 more source
Sequencing of a central nervous system tumor demonstrates cancer transmission in an organ transplant
Life Science Alliance, 2021 This study uses DNA sequencing to trace a donor organ transplant–mediated cancer transmission and illustrates how precise molecular pathology profiles might reduce future risk for transplant recipients.
Marie-Claude Gingras +15 more
doaj +1 more source
Mapping the evolution of mitochondrial complex I through structural variation
FEBS Letters, EarlyView.Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin +2 more
wiley +1 more source
Efficient Method for Molecular Characterization of the 5′ and 3′ Ends of the Dengue Virus Genome
Viruses, 2020 Dengue is a mosquito-borne disease that is of major importance in public health. Although it has been extensively studied at the molecular level, sequencing of the 5′ and 3′ ends of the untranslated regions (UTR) commonly requires specific approaches for
Alicia Rosales-Munar +4 more
doaj +1 more source
Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy [PDF]
PeerJ, 2019 Polymerase chain reaction (PCR) amplification of complex microbial genomic DNA templates with degenerate primers can lead to distortion of the underlying community structure due to inefficient primer-template interactions leading to bias.
Ankur Naqib +2 more
doaj +2 more sources
Nanopore sequencing in human genetic studies: application to structural variant detection [PDF]
, 2019 Background: Nanopore sequencing is a recent technology which allows direct real-time sequencing of DNA or RNA molecules and production of read lengths as long as the size of the original fragments.
Silva, Catarina, Vieira, Luís
core
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
npj MicrogravityThe surge in commercial and civilian spaceflight enables the systematic and longitudinal, large-scale biospecimen collection to understand the prospective effects of space travel on human health.
Aparna Krishnavajhala +17 more
doaj +1 more source
Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics [PDF]
, 2014 We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ).
Boyden, Edward S +11 more
core
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source