Results 51 to 60 of about 2,098,916 (269)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

SCORM Sequencing Testing for Sequencing Control Mode [PDF]

, 2010
In SCORM 2004 defines the sequencing information that describes how SCORM-conformant content may be sequenced to the learner through a set of learner or system-initiated navigation events.
Chang, Hsuan-Pu; Wang, Chun-Chia; Wang, Te-Hua; Jan, Kuen Han; Shih,Timothy K., Hsuan-Pu Chang; Chun-Chia Wang; Kuen Han Jan;Timothy K. Shih   +1 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Schroth Gary P, Krüger Detlev H., Kentaro Yoshida, Kahl, Guenter, Kahl, G., Fujibe, Takahiro, Krueger, Detlev H, Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Barrero, Roberto A, Yoshida Kentaro, Luo, S., Barrero Roberto A, Terauchi Ryohei, Albertyn Zayed, Luo, Shujun, Shujun Luo, Kimura, Eiji, Barrero, R.A., Schroth, Gary P., Terauchi, Ryohei, Detlev H Krüger, Yoshida Kentaro, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Yoshida, Kentaro, Kahl Günter, Matsumura, Hideo, Luo Shujun, Krueger Detlev H, Albertyn Zayed, Gary P. Schroth, Kahl Guenter, Matsumura, Hideo, Fujibe Takahiro, Matsumura Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Luo Shujun, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Albertyn, Zayed, Matsumura Hideo, Barrero Roberto A., Schroth, Gary P, Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Kimura Eiji, Roberto A. Barrero, Günter Kahl   +68 more
core   +1 more source

Routine use of microbial whole genome sequencing in diagnostic and public health microbiology [PDF]

, 2012
This work is partly supported by funding from the Medical Research Council, the European Developing Country Clinical Trials Partnership, the Global Alliance for TB Drug Development, and the European Union.Whole genome sequencing (WGS) promises to be ...
Holden, MTG, Matthew T G Holden, Mark Farrington (148224), Cartwright, EJP, Matthew T. G. Holden (130258), Brown, Nicholas M., Sharon J Peacock, Bentley, SD, Ellington, Matthew J., Claudio U Köser, Parkhill, J, Stephen D. Bentley (137281), Stephen H Gillespie, Stephen D Bentley, Köser, CU, Bentley, Stephen D., Julian Parkhill (30393), Holden, Matthew T. G., Julian Parkhill, Peacock, Sharon J., Edward J. P. Cartwright (148213), Koeser, Claudio U., Peacock, SJ, Stephen H. Gillespie (148218), Edward J P Cartwright, Gordon Dougan, Matthew J. Ellington (148208), Farrington, M, Cartwright, Edward J. P., Dougan, Gordon, Nicholas M Brown, Parkhill, Julian, Mark Farrington, Gordon Dougan (30395), Farrington, Mark, Claudio U. Köser (148202), Gillespie, Stephen H., Matthew J Ellington, Brown, NM, Sharon J. Peacock (35782), Gillespie, SH, Ellington, MJ, Nicholas M. Brown (148222), Dougan, G   +43 more
core   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

A pattern sequence approach to Stern’s sequence

Discrete Mathematics, 2011
Let w be a binary string and let a_w (n) be the number of occurrences of the word w in the binary expansion of n. As usual we let s(n) denote the Stern sequence; that is, s(0)=0, s(1)=1, and for n >= 1, s(2n)=s(n) and s(2n+1)=s(n)+s(n+1). In this note, we show that s(n) = a_1 (n) + \sum_{w in 1 (0+1)*} s([w bar]) a_{w1} (n) where w bar denotes the ...
Michael Coons, Jeffrey O. Shallit
openaire   +3 more sources

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source