Results 31 to 40 of about 470,172 (244)

Sheet-on-sheet fixed target data collection devices for serial crystallography at synchrotron and XFEL sources. [PDF]

J Appl Crystallogr
Fixed targets (‘chips’) offer efficient, high-throughput microcrystal delivery for serial crystallography at synchrotrons and X-ray free-electron lasers (XFELs).
Doak RB, Shoeman RL, Gorel A, Niziński S, Barends TRM, Schlichting I.   +5 more
europepmc   +2 more sources

All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography. [PDF]

Biomicrofluidics, 2023
The development of x-ray free electron laser (XFEL) light sources and serial crystallography methodologies has led to a revolution in protein crystallography, enabling the determination of previously unobtainable protein structures and near-atomic ...
Gu KK, Liu Z, Narayanasamy SR, Shelby ML, Chan N, Coleman MA, Frank M, Kuhl TL.   +7 more
europepmc   +2 more sources

Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography. [PDF]

Nat Commun, 2023
Photopharmacology manipulates the biological activity of small molecules by light. Using an X-ray laser, the authors follow the release of the drug azo-combretastatin A4 from tubulin and the concomitant structural changes over nine orders of magnitude in
Wranik M, Weinert T, Slavov C, Masini T, Furrer A, Gaillard N, Gioia D, Ferrarotti M, James D, Glover H, Carrillo M, Kekilli D, Stipp R, Skopintsev P, Brünle S, Mühlethaler T, Beale J, Gashi D, Nass K, Ozerov D, Johnson PJM, Cirelli C, Bacellar C, Braun M, Wang M, Dworkowski F, Milne C, Cavalli A, Wachtveitl J, Steinmetz MO, Standfuss J.   +30 more
europepmc   +2 more sources

TORO Indexer: a PyTorch-based indexing algorithm for kilohertz serial crystallography. [PDF]

J Appl Crystallogr
Serial crystallography (SX) requires efficient processing of numerous diffraction patterns. TORO Indexer is a high-performance indexing solution that operates across various platforms such as GPUs, CPUs and TPUs, offering high processing speed without ...
Gasparotto P, Barba L, Stadler HC, Assmann G, Mendonça H, Ashton AW, Janousch M, Leonarski F, Béjar B.   +8 more
europepmc   +2 more sources

A user-friendly plug-and-play cyclic olefin copolymer-based microfluidic chip for room-temperature, fixed-target serial crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2023
An improved design of an all-polymer microfluidic ‘chip’ for fixed-target serial crystallography is presented that can easily be fabricated in-house, is inexpensive and is highly modifiable to meet broad user needs for room-temperature serial ...
Liu Z, Gu KK, Shelby ML, Gilbile D, Lyubimov AY, Russi S, Cohen AE, Narayanasamy SR, Botha S, Kupitz C, Sierra RG, Poitevin F, Gilardi A, Lisova S, Coleman MA, Frank M, Kuhl TL.   +16 more
europepmc   +2 more sources

A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography. [PDF]

J Appl Crystallogr, 2023
A versatile approach for the preparation of high-density membrane protein microcrystals in lipidic cubic phase for serial crystallography is described.
Birch J, Kwan TOC, Judge PJ, Axford D, Aller P, Butryn A, Reis RI, Bada Juarez JF, Vinals J, Owen RL, Nango E, Tanaka R, Tono K, Joti Y, Tanaka T, Owada S, Sugahara M, Iwata S, Orville AM, Watts A, Moraes I.   +20 more
europepmc   +2 more sources

Data reduction for X-ray serial crystallography using machine learning. [PDF]

J Appl Crystallogr, 2023
A machine learning method for distinguishing good and bad images in serial crystallography is presented. To reduce the computational cost, this uses the oriented FAST and rotated BRIEF feature extraction method from computer vision to detect image ...
Rahmani V, Nawaz S, Pennicard D, Setty SPR, Graafsma H.   +4 more
europepmc   +2 more sources

Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography. [PDF]

Nat Commun
G protein-coupled receptors (GPCRs) are the largest family of cell surface receptors in humans. The binding and dissociation of ligands tunes the inherent conformational flexibility of these important drug targets towards distinct functional states. Here
Glover H, Saßmannshausen T, Bertrand Q, Trabuco M, Slavov C, Bacchin A, Andres F, Kondo Y, Stipp R, Wranik M, Khusainov G, Carrillo M, Kekilli D, Nan J, Gonzalez A, Cheng R, Neidhart W, Weinert T, Leonarski F, Dworkowski F, Kepa M, Wachtveitl J, Hennig M, Standfuss J.   +23 more
europepmc   +2 more sources

Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome <i>c</i> oxidase. [PDF]

Sci Adv, 2023
Cytochrome c oxidase (CcO) is part of the respiratory chain and contributes to the electrochemical membrane gradient in mitochondria as well as in many bacteria, as it uses the energy released in the reduction of oxygen to pump protons across an energy ...
Safari C, Ghosh S, Andersson R, Johannesson J, Båth P, Uwangue O, Dahl P, Zoric D, Sandelin E, Vallejos A, Nango E, Tanaka R, Bosman R, Börjesson P, Dunevall E, Hammarin G, Ortolani G, Panman M, Tanaka T, Yamashita A, Arima T, Sugahara M, Suzuki M, Masuda T, Takeda H, Yamagiwa R, Oda K, Fukuda M, Tosha T, Naitow H, Owada S, Tono K, Nureki O, Iwata S, Neutze R, Brändén G.   +35 more
europepmc   +2 more sources

A snapshot love story: what serial crystallography has done and will do for us. [PDF]

Acta Crystallogr D Struct Biol
This article gives an overview of current trends and challenges in serial crystallography, with a focus on results from time-resolved experiments.
Henkel A, Oberthür D.
europepmc   +2 more sources