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Standard Sample Preparation for Serial Femtosecond Crystallography [PDF]

Biomolecules
The development of serial crystallography (SX), including serial synchrotron crystallography (SSX) at synchrotron sources and serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs), has facilitated the collection of high ...
Christina Schmidt, Kristina Lorenzen, Joachim Schulz, Huijong Han   +3 more
doaj   +3 more sources

Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE [PDF]

Structural Dynamics
X-ray Free Electron Lasers (XFELs) allow the collection of high-quality serial femtosecond crystallography data. The next generation of megahertz superconducting FELs promises to drastically reduce data collection times, enabling the capture of more ...
Samuel Perrett, Alisia Fadini, Christopher D. M. Hutchison, Sayantan Bhattacharya, Cade Morrison, Oleksii Turkot, Mads Bregenholt Jakobsen, Michael Größler, José Licón-Saláiz, Florian Griese, Samuel Flewett, Joana Valerio, Joachim Schulz, Mykola Biednov, Yifeng Jiang, Huijong Han, Hazem Yousef, Dmitry Khakhulin, Christopher Milne, Anton Barty, Jasper J. van Thor   +20 more
doaj   +4 more sources

Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals [PDF]

IUCrJ, 2021
The crystallization of recombinant proteins in living cells is an exciting new approach in structural biology. Recent success has highlighted the need for fast and efficient diffraction data collection, optimally directly exposing intact crystal ...
J. Mia Lahey-Rudolph, Robert Schönherr, Miriam Barthelmess, Pontus Fischer, Carolin Seuring, Armin Wagner, Alke Meents, Lars Redecke   +7 more
doaj   +6 more sources

Room temperature structures beyond 1.5 Å by serial femtosecond crystallography [PDF]

Structural Dynamics, 2015
About 2.5 × 106 snapshots on microcrystals of photoactive yellow protein (PYP) from a recent serial femtosecond crystallographic (SFX) experiment were reanalyzed to maximum resolution.
Marius Schmidt, Kanupriya Pande, Shibom Basu, Jason Tenboer   +3 more
doaj   +5 more sources

Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography [PDF]

Applied Sciences, 2022
Serial femtosecond crystallography (SFX) using an X-ray free-electron laser (XFEL) can be applied to determine the room-temperature structure of target molecules while minimizing radiation damage and visualizing molecular dynamics.
Keondo Lee, Donghyeon Lee, Jaehyun Park, Jong-Lam Lee, Wan Kyun Chung, Yunje Cho, Ki Hyun Nam   +6 more
doaj   +2 more sources

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation [PDF]

IUCrJ, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall   +26 more
doaj   +9 more sources

A fixed-target platform for serial femtosecond crystallography in a hydrated environment. [PDF]

IUCrJ, 2020
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while ...
Shelby ML, Gilbile D, Grant TD, Seuring C, Segelke BW, He W, Evans AC, Pakendorf T, Fischer P, Hunter MS, Batyuk A, Barthelmess M, Meents A, Coleman MA, Kuhl TL, Frank M.   +15 more
europepmc   +3 more sources

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin [PDF]

eLife, 2021
Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as ...
Kazumasa Oda, Takashi Nomura, Takanori Nakane, Keitaro Yamashita, Keiichi Inoue, Shota Ito, Johannes Vierock, Kunio Hirata, Andrés D Maturana, Kota Katayama, Tatsuya Ikuta, Itsuki Ishigami, Tamaki Izume, Rie Umeda, Ryuun Eguma, Satomi Oishi, Go Kasuya, Takafumi Kato, Tsukasa Kusakizako, Wataru Shihoya, Hiroto Shimada, Tomoyuki Takatsuji, Mizuki Takemoto, Reiya Taniguchi, Atsuhiro Tomita, Ryoki Nakamura, Masahiro Fukuda, Hirotake Miyauchi, Yongchan Lee, Eriko Nango, Rie Tanaka, Tomoyuki Tanaka, Michihiro Sugahara, Tetsunari Kimura, Tatsuro Shimamura, Takaaki Fujiwara, Yasuaki Yamanaka, Shigeki Owada, Yasumasa Joti, Kensuke Tono, Ryuichiro Ishitani, Shigehiko Hayashi, Hideki Kandori, Peter Hegemann, So Iwata, Minoru Kubo, Tomohiro Nishizawa, Osamu Nureki   +47 more
doaj   +2 more sources

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography [PDF]

Nature Communications, 2021
MAL and MyD88 are downstream adaptors of Toll-like receptors (TLR) and the MAL TIR domain forms filaments in vitro, which in turn nucleate the assembly of crystalline arrays of the MyD88 TIR domain.
Max T. B. Clabbers, Susannah Holmes, Timothy W. Muusse, Parimala R. Vajjhala, Sara J. Thygesen, Alpeshkumar K. Malde, Dominic J. B. Hunter, Tristan I. Croll, Leonie Flueckiger, Jeffrey D. Nanson, Md. Habibur Rahaman, Andrew Aquila, Mark S. Hunter, Mengning Liang, Chun Hong Yoon, Jingjing Zhao, Nadia A. Zatsepin, Brian Abbey, Emma Sierecki, Yann Gambin, Katryn J. Stacey, Connie Darmanin, Bostjan Kobe, Hongyi Xu, Thomas Ve   +24 more
doaj   +2 more sources

Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre. [PDF]

Acta Crystallogr D Struct Biol, 2022
Båth P, Banacore A, Börjesson P, Bosman R, Wickstrand C, Safari C, Dods R, Ghosh S, Dahl P, Ortolani G, Björg Ulfarsdottir T, Hammarin G, García Bonete MJ, Vallejos A, Ostojić L, Edlund P, Linse JB, Andersson R, Nango E, Owada S, Tanaka R, Tono K, Joti Y, Nureki O, Luo F, James D, Nass K, Johnson PJM, Knopp G, Ozerov D, Cirelli C, Milne C, Iwata S, Brändén G, Neutze R.   +34 more
europepmc   +3 more sources