Results 11 to 20 of about 4,136 (214)

Double-flow focused liquid injector for efficient serial femtosecond crystallography [PDF]

Scientific Reports, 2017
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment.
Beyerlein, Kenneth R., Bushnell, David A., Knoska, Juraj, Kovaleva, Elena G. [u.v.m.], Oberthuer, Dominik, Szczepek, Michael, Wiedorn, Max O.   +6 more
core   +9 more sources

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase [PDF]

IUCrJ, 2015
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage.
Raimund Fromme, Andrii Ishchenko, Markus Metz, Shatabdi Roy Chowdhury, Shibom Basu, Sébastien Boutet, Petra Fromme, Thomas A. White, Anton Barty, John C. H. Spence, Uwe Weierstall, Wei Liu, Vadim Cherezov   +12 more
doaj   +6 more sources

Experimental phasing of serial femtosecond crystallography data [PDF]

IUCrJ, 2017
A synopsis of and prospects for de novo phasing using diffraction data collected at X-ray free-electron lasers are given.
Ilme Schlichting
doaj   +6 more sources

Asymmetry in serial femtosecond crystallography data. [PDF]

Acta Crystallogr A Found Adv, 2017
Serial crystallography is an increasingly important approach to protein crystallography that exploits both X-ray free-electron laser (XFEL) and synchrotron radiation. Serial crystallography recovers complete X-ray diffraction data by processing and merging diffraction images from thousands of randomly oriented non-uniform microcrystals, of which all ...
Sharma A, Johansson L, Dunevall E, Wahlgren WY, Neutze R, Katona G.   +5 more
europepmc   +6 more sources

A Bright Future for Serial Femtosecond Crystallography with XFELs. [PDF]

Trends Biochem Sci, 2017
X-ray free electron lasers (XFELs) have the potential to revolutionize macromolecular structural biology due to the unique combination of spatial coherence, extreme peak brilliance, and short duration of X-ray pulses. A recently emerged serial femtosecond (fs) crystallography (SFX) approach using XFEL radiation overcomes some of the biggest hurdles of ...
Johansson LC, Stauch B, Ishchenko A, Cherezov V.   +3 more
europepmc   +6 more sources

Serial femtosecond crystallography: the first five years [PDF]

IUCrJ, 2015
Protein crystallography using synchrotron radiation sources has had a tremendous impact on biology, having yielded the structures of thousands of proteins and given detailed insight into their mechanisms.
Ilme Schlichting
doaj   +4 more sources

Chemical crystallography by serial femtosecond X-ray diffraction [PDF]

Nature, 2022
AbstractInorganic–organic hybrid materials represent a large share of newly reported structures, owing to their simple synthetic routes and customizable properties1. This proliferation has led to a characterization bottleneck: many hybrid materials are obligate microcrystals with low symmetry and severe radiation sensitivity, interfering with the ...
Elyse A. Schriber⧓, Daniel W. Paley⧓, Robert Bolotovsky, Daniel J. Rosenberg, Raymond G. Sierra, Andrew Aquila, Derek Mendez, Frédéric Poitevin, Johannes Blaschke, Asmit Bhowmick, Ryan P. Kelly, Mark S. Hunter, Brandon Hayes, Derek Popple, Matthew Yeung, Carina Pareja‐Rivera, Stella Lisova, Kensuke Tono, Michihiro Sugahara, Shigeki Owada, Tevye Kuykendall, Kaiyuan Yao, P. James Schuck, Diego Solís-Ibarra, Nicholas K. Sauter, Aaron S. Brewster, J. Nathan Hohman   +26 more
  +8 more sources

Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography [PDF]

IUCrJ
Serial femtosecond crystallography (SFX) exploits extremely brief X-ray free-electron laser pulses to obtain diffraction data before destruction of the crystal.
Lewis J. Williams, Amy J. Thompson, Philipp Dijkstal, Martin Appleby, Greta Assmann, Florian S. N. Dworkowski, Nicole Hiller, Chia-Ying Huang, Tom Mason, Samuel Perrett, Eduard Prat, Didier Voulot, Bill Pedrini, John H. Beale, Michael A. Hough, Jonathan A. R. Worrall, Robin L. Owen   +16 more
doaj   +2 more sources

Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide [PDF]

IUCrJ
Time-resolved X-ray crystallography is undergoing a renaissance due to the development of serial crystallography at synchrotron and XFEL beamlines. Crucial to such experiments are efficient and effective methods for uniformly initiating time-dependent ...
Peter Smyth, Sofia Jaho, Lewis J. Williams, Gabriel Karras, Ann Fitzpatrick, Amy J. Thompson, Sinan Battah, Danny Axford, Sam Horrell, Marina Lučić, Kotone Ishihara, Machika Kataoka, Hiroaki Matsuura, Kanji Shimba, Kensuke Tono, Takehiko Tosha, Hiroshi Sugimoto, Shigeki Owada, Michael A. Hough, Jonathan A.R. Worrall, Robin L. Owen   +20 more
doaj   +2 more sources

Single-molecule imaging with longer X-ray laser pulses [PDF]

IUCrJ, 2015
During the last five years, serial femtosecond crystallography using X-ray laser pulses has been developed into a powerful technique for determining the atomic structures of protein molecules from micrometre- and sub-micrometre-sized crystals. One of the
Andrew V. Martin, Justine K. Corso, Carl Caleman, Nicusor Timneanu, Harry M. Quiney   +4 more
doaj   +6 more sources