Results 21 to 30 of about 4,136 (214)
Hydroxyethyl cellulose matrix applied to serial crystallography [PDF]
Scientific Reports, 2017 Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the ...Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata +18 moredoaj +2 more sourcesSerial femtosecond crystallography. [PDF]
Nat Rev Methods Primers, 2022 With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing ...Barends TRM, Stauch B, Cherezov V, Schlichting I. +3 moreeuropepmc +5 more sourcesSerial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade [PDF]
Nature Communications, 2020 Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond ...Guillaume Tetreau, Anne-Sophie Banneville, Elena A. Andreeva, Aaron S. Brewster, Mark S. Hunter, Raymond G. Sierra, Jean-Marie Teulon, Iris D. Young, Niamh Burke, Tilman A. Grünewald, Joël Beaudouin, Irina Snigireva, Maria Teresa Fernandez-Luna, Alister Burt, Hyun-Woo Park, Luca Signor, Jayesh A. Bafna, Rabia Sadir, Daphna Fenel, Elisabetta Boeri-Erba, Maria Bacia, Ninon Zala, Frédéric Laporte, Laurence Després, Martin Weik, Sébastien Boutet, Martin Rosenthal, Nicolas Coquelle, Manfred Burghammer, Duilio Cascio, Michael R. Sawaya, Mathias Winterhalter, Enrico Gratton, Irina Gutsche, Brian Federici, Jean-Luc Pellequer, Nicholas K. Sauter, Jacques-Philippe Colletier +37 moredoaj +2 more sourcesPink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser. [PDF]
IUCrJ, 2021 Nass K, Bacellar C, Cirelli C, Dworkowski F, Gevorkov Y, James D, Johnson PJM, Kekilli D, Knopp G, Martiel I, Ozerov D, Tolstikova A, Vera L, Weinert T, Yefanov O, Standfuss J, Reiche S, Milne CJ. +17 moreeuropepmc +3 more sourcesDetection of a Geminate Photoproduct of Bovine Cytochrome c Oxidase by Time-Resolved Serial Femtosecond Crystallography. [PDF]
J Am Chem Soc, 2023 Ishigami I, Carbajo S, Zatsepin N, Hikita M, Conrad CE, Nelson G, Coe J, Basu S, Grant T, Seaberg MH, Sierra RG, Hunter MS, Fromme P, Fromme R, Rousseau DL, Yeh SR. +15 moreeuropepmc +3 more sourcesA novel inert crystal delivery medium for serial femtosecond crystallography [PDF]
IUCrJ, 2015 Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins.Chelsie E. Conrad, Shibom Basu, Daniel James, Dingjie Wang, Alexander Schaffer, Shatabdi Roy-Chowdhury, Nadia A. Zatsepin, Andrew Aquila, Jesse Coe, Cornelius Gati, Mark S. Hunter, Jason E. Koglin, Christopher Kupitz, Garrett Nelson, Ganesh Subramanian, Thomas A. White, Yun Zhao, James Zook, Sébastien Boutet, Vadim Cherezov, John C. H. Spence, Raimund Fromme, Uwe Weierstall, Petra Fromme +23 moredoaj +5 more sourcesMegahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers. [PDF]
Nat Commun, 2022 Holmes S, Kirkwood HJ, Bean R, Giewekemeyer K, Martin AV, Hadian-Jazi M, Wiedorn MO, Oberthür D, Marman H, Adriano L, Al-Qudami N, Bajt S, Barák I, Bari S, Bielecki J, Brockhauser S, Coleman MA, Cruz-Mazo F, Danilevski C, Dörner K, Gañán-Calvo AM, Graceffa R, Fanghor H, Heymann M, Frank M, Kaukher A, Kim Y, Kobe B, Knoška J, Laurus T, Letrun R, Maia L, Messerschmidt M, Metz M, Michelat T, Mills G, Molodtsov S, Monteiro DCF, Morgan AJ, Münnich A, Peña Murillo GE, Previtali G, Round A, Sato T, Schubert R, Schulz J, Shelby M, Seuring C, Sellberg JA, Sikorski M, Silenzi A, Stern S, Sztuk-Dambietz J, Szuba J, Trebbin M, Vagovic P, Ve T, Weinhausen B, Wrona K, Xavier PL, Xu C, Yefanov O, Nugent KA, Chapman HN, Mancuso AP, Barty A, Abbey B, Darmanin C. +67 moreeuropepmc +3 more sourcesSerial femtosecond crystallography structure of cytochrome c oxidase at room temperature [PDF]
Scientific Reports, 2017 Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane.Rebecka Andersson, Cecilia Safari, Robert Dods, Eriko Nango, Rie Tanaka, Ayumi Yamashita, Takanori Nakane, Kensuke Tono, Yasumasa Joti, Petra Båth, Elin Dunevall, Robert Bosman, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén +15 moredoaj +2 more sourcesObservation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography. [PDF]
Commun ChemMalla TN, Aldama L, Leon V, Feliz D, Hu H, Thomas I, Cellini A, Wahlgren WY, Nimmrich A, Botha S, Sierra R, Hunter MS, Poitevin F, Lisova S, Batyuk A, Gate G, Jernigan R, Kupitz CJ, Maj P, Meszaros P, Kurttila M, Monrroy L, Luo F, Owada S, Kang J, Slavov C, Maj M, Gautier C, Kashipathy M, Tolstikova A, Mariani V, Barty A, Moss F, Schwander P, Liu H, Boutet S, Fromme P, Takala H, Ihalainen JA, Weierstall U, Westenhoff S, Stojković EA, Schmidt M. +42 moreeuropepmc +3 more sourcesReal-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography. [PDF]
Nat CommunMaity B, Shoji M, Luo F, Nakane T, Abe S, Owada S, Kang J, Tono K, Tanaka R, Pham TT, Kojima M, Hishikawa Y, Tanaka J, Tian J, Nagama M, Suzuki T, Noya H, Nakasuji Y, Asanuma A, Yao X, Iwata S, Shigeta Y, Nango E, Ueno T. +23 moreeuropepmc +3 more sources
