Results 41 to 50 of about 4,136 (214)

Polyacrylamide injection matrix for serial femtosecond crystallography. [PDF]

Sci Rep, 2019
AbstractSerial femtosecond crystallography (SFX) provides opportunities to observe the dynamics of macromolecules without causing radiation damage at room temperature. Although SFX provides a biologically more reliable crystal structure than provided by the existing synchrotron sources, there are limitations due to the consumption of many crystal ...
Park J, Park S, Kim J, Park G, Cho Y, Nam KH.   +5 more
europepmc   +4 more sources

Weakly supervised learning for pattern classification in serial femtosecond crystallography

Optics Express, 2023
Serial femtosecond crystallography at X-ray free electron laser facilities opens a new era for the determination of crystal structure. However, the data processing of those experiments is facing unprecedented challenge, because the total number of diffraction patterns needed to determinate a high-resolution structure is huge.
Jianan Xie, Ji Liu, Chi Zhang, Xihui Chen, Ping Huai, Jie Zheng, Xiaofeng Zhang   +6 more
openalex   +4 more sources

Lipidic phase membrane protein serial femtosecond crystallography [PDF]

Nature Methods, 2012
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center ...
Johansson L. C., Arnlund D., White T. A., Katona G., Deponte D. P., Weierstall U., Doak R. B., Shoeman R. L., Lomb L., Malmerberg E., Davidsson J., Nass K., Liang M., Andreasson J., Aquila A., Bajt S., Barthelmess M., Barty A., Bogan M. J., Bostedt C., Bozek J. D., Caleman C., Coffee R., Coppola N., Ekeberg T., Epp S. W., Erk B., Fleckenstein H., Foucar L., Graafsma H., Gumprecht L., Hajdu J., Hampton C. Y., Hartmann R., Hartmann A., Hauser G., Hirsemann H., Holl P., Hunter M. S., Kassemeyer S., Kimmel N., Kirian R. A., Maia F. R. N. C., Marchesini S., Martin A. V., Reich C., Rolles D., Rudek B., Rudenko A., Schlichting I., Schulz J., Seibert M. M., Sierra R. G., Soltau H., Starodub D., Stellato F., Stern S., Strulder L., Timneanu N., Ullrich J., Wahlgren W. Y., Wang X., Weidenspointner G., Wunderer C., Fromme P., Chapman H. N., Spence J. C. H., Neutze R.   +67 more
openaire   +8 more sources

Nanoflow electrospinning serial femtosecond crystallography [PDF]

Acta Crystallographica Section D Biological Crystallography, 2012
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or ...
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J.   +37 more
openaire   +2 more sources

Serial X-ray Crystallography

Crystals, 2022
Serial crystallography (SX) is an emerging technique to determine macromolecules at room temperature. SX with a pump–probe experiment provides the time-resolved dynamics of target molecules.
Ki Hyun Nam
doaj   +1 more source

The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals. [PDF]

Curr Opin Struct Biol, 2019
Zatsepin NA, Li C, Colasurd P, Nannenga BL.   +3 more
europepmc   +3 more sources

Serial femtosecond crystallography datasets from G protein-coupled receptors [PDF]

Scientific Data, 2016
AbstractWe describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with
Thomas A. White, Anton Barty, Wei Liu, Andrii Ishchenko, Haitao Zhang, Cornelius Gati, Nadia A. Zatsepin, Shibom Basu, Dominik Oberthür, Markus Metz, Kenneth R. Beyerlein, Chun Hong Yoon, Oleksandr Yefanov, Daniel James, Dingjie Wang, M. Messerschmidt, Jason E. Koglin, Sébastien Boutet, Uwe Weierstall, Vadim Cherezov   +19 more
openalex   +5 more sources

3D printed devices and infrastructure for liquid sample delivery at the European XFEL

Journal of Synchrotron Radiation, 2022
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Mohammad Vakili, Johan Bielecki, Juraj Knoška, Florian Otte, Huijong Han, Marco Kloos, Robin Schubert, Elisa Delmas, Grant Mills, Raphael de Wijn, Romain Letrun, Simon Dold, Richard Bean, Adam Round, Yoonhee Kim, Frederico A. Lima, Katerina Dörner, Joana Valerio, Michael Heymann, Adrian P. Mancuso, Joachim Schulz   +20 more
doaj   +1 more source

Serial Femtosecond Crystallography of G Protein-Coupled Receptors. [PDF]

Annu Rev Biophys, 2018
G protein–coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in
Stauch B, Cherezov V.
europepmc   +6 more sources

Serial Femtosecond Crystallography of G Protein–Coupled Receptors [PDF]

Science, 2013
G Structures G protein–coupled receptors (GPCRs) are eukaryotic membrane proteins that have a central role in cellular communication and have become key drug targets. To overcome the difficulties of growing GPCRs crystals, Liu et al.
W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. A. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. H. Spence, R. C. Stevens, V. Cherezov   +32 more
openaire   +3 more sources