Results 61 to 70 of about 4,136 (214)

Entering an era of dynamic structural biology…

BMC Biology, 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
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Serial crystallography is just getting started [PDF]

Structural Dynamics
When serial crystallography at XFELs was first being developed, it was unclear how it should best be used. Early experiments included studying single crystals, or weak or small crystals, or sample delivery methods that over used crystal material or under
Aaron S Brewster, Daniel W Paley, David W Mittan-Moreau, Nicholas K Sauter   +3 more
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High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography [PDF]

Science, 2012
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth of large crystals, in part because a sufficient number of molecules must be present in the lattice for the sample to withstand ...
Boutet S., Lomb L., Williams G. J., Barends T. R. M., Aquila A., Doak R. B., Weierstall U., DePonte D. P., Steinbrener J., Shoeman R. L., Messerschmidt M., Barty A., White T. A., Kassemeyer S., Kirian R. A., Seibert M. M., Montanez P. A., Kenney C., Herbst R., Hart P., Pines J., Haller G., Gruner S. M., Philipp H. T., Tate M. W., Hromalik M., Koerner L. J., Van Bakel N., Morse J., Ghonsalves W., Arnlund D., Bogan M. J., Caleman C., Fromme R., Hampton C. Y., Hunter M. S., Johansson L. C., Katona G., Kupitz C., Liang M., Martin A. V., Nass K., Redecke L., Stellato F., Timneanu N., Wang D., Zatsepin N. A., Schafer D., Defever J., Neutze R., Fromme P., Spence J. C. H., Chapman H. N., Schlichting I.   +53 more
openaire   +6 more sources

Cooperative allostery and structural dynamics of streptavidin at cryogenic- and ambient-temperature

Communications Biology, 2022
Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature.
Esra Ayan, Busra Yuksel, Ebru Destan, Fatma Betul Ertem, Gunseli Yildirim, Meryem Eren, Oleksandr M. Yefanov, Anton Barty, Alexandra Tolstikova, Gihan K. Ketawala, Sabine Botha, E. Han Dao, Brandon Hayes, Mengning Liang, Matthew H. Seaberg, Mark S. Hunter, Alexander Batyuk, Valerio Mariani, Zhen Su, Frederic Poitevin, Chun Hong Yoon, Christopher Kupitz, Aina Cohen, Tzanko Doukov, Raymond G. Sierra, Çağdaş Dağ, Hasan DeMirci   +26 more
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Towards the spatial resolution of metalloprotein charge states by detailed modeling of XFEL crystallographic diffraction. [PDF]

, 2020
Oxidation states of individual metal atoms within a metalloprotein can be assigned by examining X-ray absorption edges, which shift to higher energy for progressively more positive valence numbers.
Holton, James M, Kern, Jan, Sauter, Nicholas K, Yano, Junko   +3 more
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Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural Dynamics, 2015
Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy.
Valérie Panneels, Wenting Wu, Ching-Ju Tsai, Przemek Nogly, Jan Rheinberger, Kathrin Jaeger, Gregor Cicchetti, Cornelius Gati, Leonhard M. Kick, Leonardo Sala, Guido Capitani, Chris Milne, Celestino Padeste, Bill Pedrini, Xiao-Dan Li, Jörg Standfuss, Rafael Abela, Gebhard Schertler   +17 more
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High-speed fixed-target serial virus crystallography [PDF]

, 2017
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with
Alonso-Mori, R, Brewster, AS, Damiani, DS, David, C, Duman, R, Fischer, P, Fry, EE, Ginn, HM, Harlos, K, Kelly, J, Kotecha, A, Meents, A, Meyer, J, Michels-Clark, T, Nelson, S, Pakendorf, T, Reime, B, Ren, J, Roedig, P, Rowlands, DJ, Sauter, NK, Sikorsky, M, Stuart, DI, Sutton, G, Vartiainen, I, Wagner, A, Walter, TS, Warmer, M, Young, ID   +28 more
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Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography [PDF]

Cell, 2015
Angiotensin II type 1 receptor (AT(1)R) is a G protein-coupled receptor that serves as a primary regulator for blood pressure maintenance. Although several anti-hypertensive drugs have been developed as AT(1)R blockers (ARBs), the structural basis for AT(1)R ligand-binding and regulation has remained elusive, mostly due to the difficulties of growing ...
Haitao Zhang, Hamiyet Ünal, Cornelius Gati, Gye Won Han, Wei Liu, Nadia A. Zatsepin, Daniel James, Dingjie Wang, Garrett Nelson, Uwe Weierstall, M.R. Sawaya, Qingping Xu, M. Messerschmidt, Garth J. Williams, Sébastien Boutet, Oleksandr Yefanov, Thomas A. White, Chong Wang, Andrii Ishchenko, Kalyan Tirupula, Russell Desnoyer, Jesse Coe, Chelsie E. Conrad, Petra Fromme, Raymond C. Stevens, Vsevolod Katritch, Sadashiva S. Karnik, Vadim Cherezov   +27 more
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An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography. [PDF]

, 2015
SACLAのX線自由電子レーザーを用いた新規タンパク質立体構造決定に世界で初めて成功. 京都大学プレスリリース. 2015-09-14.Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to ...
Ago, Hideo, Gomi, Keiko, Hatsui, Takaki, Iwata, So, Joti, Yasumasa, Kajiyama, Naoki, Kameshima, Takashi, Kato, Hiroaki, Kodan, Atsushi, Mizohata, Eiichi, Murai, Tomohiro, Nakatsu, Toru, Nango, Eriko, Okuda, Tomohiko, Pan, Dongqing, Park, Jaehyun, Song, Changyong, Sugahara, Michihiro, Suzuki, Mamoru, Tono, Kensuke, Yabashi, Makina, Yamaguchi, Tomohiro, Yamamoto, Masaki, Yamashita, Keitaro   +23 more
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Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nature Communications, 2016
Serial femtosecond crystallography using X-ray free-electron lasers has huge potential for time-resolved structural experiments. Here, the authors present a structure of the light-driven proton pump bacteriorhodopsin using these techniques.
Przemyslaw Nogly, Valerie Panneels, Garrett Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie Conrad, Jesse Coe, Richard Bean, Yun Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, Kenneth R. Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonte, Chufeng Li, Leonardo Sala, Garth J. Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry N. Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard Schertler, Jörg Standfuss   +39 more
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