Results 121 to 130 of about 3,653 (216)

Unraveling the active site cover of coproheme decarboxylase from Listeria monocytogenes

The FEBS Journal, EarlyView.
During heme biosynthesis in Gram‐positive bacteria, coproheme decarboxylase (ChdC) catalyzes the conversion of four‐propionate substrate coproheme to the two propionate product heme b. Its active site is universally covered by a flexible linking loop. This study identifies an important histidine residue, which stabilizes the loop in a ChdC homolog.
Nikolaus Falb, Dominik Hörtnagel, Chris Oostenbrink, Paul G. Furtmüller, Stefan Hofbauer   +4 more
wiley   +1 more source

A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst

Nature Communications
With the advent of serial X-ray crystallography on microfocus beamlines at free-electron laser and synchrotron facilities, the demand for protein microcrystals has significantly risen in recent years.
Robert Schönherr, Juliane Boger, J. Mia Lahey-Rudolph, Mareike Harms, Jacqueline Kaiser, Sophie Nachtschatt, Marla Wobbe, Rainer Duden, Peter König, Gleb Bourenkov, Thomas R. Schneider, Lars Redecke   +11 more
doaj   +1 more source

Experimental phasing with serial crystallography at XFEL and synchrotron radiation [PDF]

Acta Crystallographica Section A Foundations and Advances, 2016
Keitaro Yamashita, Kazuya Hasegawa, Dongqing Pan, Tomohiro Murai, Kunio Hirata, Go Ueno, Toru Nakatsu, Hideo Ago, Takashi Kumasaka, Masaki Yamamoto   +9 more
openaire   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

Defining the role of αC helix interactions in the activation of the integrin αI domain

The FEBS Journal, EarlyView.
The α2I domain of the α2β1 integrin contains a distinctive αC helix and a conserved Arg288‐Glu318 ion pair that stabilizes the closed state of the domain. Disruption of this ion pair in the activated α2I variant unwinds the αC helix, which allows interaction with additional collagen‐binding sites increasing collagen binding.
Liisa Pösö, Tomi T. Airenne, Jarmo Käpylä, Hongmin Tu, Eva Bligt‐Lindén, Vimal Parkash, Jyrki Heino, Tiina A. Salminen   +7 more
wiley   +1 more source

Latest advances in serial crystallography at XFELs and synchrotron sources [PDF]

Acta Crystallographica Section A Foundations and Advances, 2019
Jose Martin-Garcia, John Spence, Petra Fromme   +2 more
openaire   +1 more source

Structural insights into xyloglucan recognition by an ABC transporter from a Gram‐positive, thermophilic bacterium

The FEBS Journal, EarlyView.
Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo, Virginia Jiang, Philip D. Jeffrey, Angela Zhu, A. James Link, Jerelle A. Joseph, Jonathan M. Conway   +6 more
wiley   +1 more source

When biology meets materials science – Interdisciplinary applications of electron microscopy

Journal of Microscopy, EarlyView.
Abstract Research at the interface between biology and materials science creates challenges for electron microscopists. Everything from the sample preparation to the choice of imaging and analytical techniques and the interpretation of the resulting data refuses to sit comfortably within the domain of one discipline or the other.
Martin Saunders, Peta L. Clode, Enoch V. S. Wong, Anna‐Lee Jessop, Gerd E. Shröder‐Turk, Felix de Tombeur   +5 more
wiley   +1 more source

Novel Amino Acid Changes Increase Phosphoenolpyruvate Carboxylase Output in planta

Plant, Cell &Environment, EarlyView.
ABSTRACT Characterization of previously reported Arabidopsis mutants with increased aluminum (Al) resistance (alr) resulting from greater release of Al‐chelating malate identified three unique amino acid substitutions that each impacts PHOSPHOENOLPYRUVATE CARBOXYLASE 1, a key anaplerotic carbon fixation enzyme.
Jinrui Sheng, Kevin Frausto, Sarah Haesaerts, Taylor J. Meyer, Maruša Prolič‐Kalinšek, Seán E. O'Leary, Remy Loris, Paul B. Larsen   +7 more
wiley   +1 more source

Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography. [PDF]

Nat Commun
Bosman R, Ortolani G, Ghosh S, James D, Norder P, Hammarin G, Úlfarsdóttir TB, Ostojić L, Weinert T, Dworkowski F, Tomizaki T, Standfuss J, Brändén G, Neutze R.   +13 more
europepmc   +1 more source