Results 131 to 140 of about 3,647 (218)

MIDAS: a methodological framework for high‐speed high‐energy diffraction microscopy data reduction. Part I: methodology

Acta Crystallographica Section A, EarlyView.
This paper details the complete methodological framework implemented in the MIDAS software for processing high‐energy diffraction microscopy (HEDM) data. We describe the specific algorithms, coordinate systems and physical models used for both far‐field and near‐field HEDM analysis.
Hemant Sharma, Jun-Sang Park, Peter Kenesei   +2 more
wiley   +1 more source

Crystallography in Open Science and its open educational resources

Acta Crystallographica Section A, EarlyView.
Open Science is the movement to make scientific research, the data and their dissemination available to any member of an inquiring society, from professionals to citizens, irrespective of their economic situation. The IUCr provides fully open educational resources to Global South and Global North readers and authors.This article presents a review of ...
John R. Helliwell
wiley   +1 more source

Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography. [PDF]

Nat Commun
Bosman R, Ortolani G, Ghosh S, James D, Norder P, Hammarin G, Úlfarsdóttir TB, Ostojić L, Weinert T, Dworkowski F, Tomizaki T, Standfuss J, Brändén G, Neutze R.   +13 more
europepmc   +1 more source

Assessing protein‐specific radiation damage in time‐resolved X‐ray solution‐scattering experiments at high‐brilliance synchrotrons using fast detector readout

Acta Crystallographica Section D, EarlyView.
Adenylate kinase was used to quantify how experimental parameters influence radiation damage during time‐resolved X‐ray solution scattering (TR‐XSS) at a multipurpose SAXS beamline. The study provides general guidelines for minimizing radiation artifacts in TR‐XSS.Time‐resolved X‐ray solution scattering (TR‐XSS) enables tracking of protein structural ...
Fatemeh Sabzian-Molaei, Tomás S. Plivelic, Magnus Andersson   +2 more
wiley   +1 more source

Biochemical and structural characterization of a tail‐spike protein with depolymerase activity identified in a marine podovirus

Acta Crystallographica Section D, EarlyView.
The marine tail‐spike protein Dpo31 degrades the exopolysaccharide of its host and has structural features similar to those of other members of this protein class, despite similarity not being detected at the sequence level.Marine phages are, through the infection of their bacterial hosts, key regulators of microbiome and carbon fluxes in the ocean ...
Serena Sirigu, Thomas Roret, Pierre-Yves Mocaër, Robert Larocque, Diane Jouanneau, Pierre Legrand, Anne-Claire Baudoux, Mirjam Czjzek   +7 more
wiley   +1 more source

Structural basis of regioselective double halogenation of the β‐carboline tryptoline by the single‐component halogenase AetF

Acta Crystallographica Section D, EarlyView.
A structure of the flavin‐dependent single‐component tryptophan halogenase AetF bound to the non‐native tricyclic substrate tryptoline reveals a binding pose analogous to l‐tryptophan that directs C6‐first halogenation. Product analysis identifies 6,8‐dibromotryptoline as the final product.Flavin‐dependent halogenases (FDHs) offer a biocatalytic route ...
Simon Bork, Hendrik J. Horstmeier, Bjarne Scharkowski, Hartmut H. Niemann   +3 more
wiley   +1 more source

Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography

, 2023
Gotthard G, Mous S, Weinert T, Antonelli Maia RN, James D, Dworkowski F, Gashi D, Furrer A, Ozerov D, Panepucci E, Wang M, Schertler GFX, Heberle J, Standfuss J, Nogly P.   +14 more
europepmc   +1 more source

Anemia‐associated mutations disrupt the CDIN1‐Codanin1 complex in inherited congenital dyserythropoietic anemia I (CDA‐I) disease

The FEBS Journal, EarlyView.
Congenital dyserythropoietic anemia type I (CDA‐I) arises from mutations in Codanin1 and CDIN1. Using quantitative biophysical approaches, we show that disease‐associated mutations disrupt the CDIN1‐Codanin1 complex. Our findings provide critical insights into the molecular mechanism that links protein dysfunction to disturbing chromatin arrangement ...
Martin Stojaspal, Tomas Brom, Ivona Nečasová, Tomáš Janovič, Pavel Veverka, Naina Verma, Lukáš Uhrík, Lenka Hernychova, Ctirad Hofr   +8 more
wiley   +1 more source

Water, water, every where—the advent of hydrated pili structure

The FEBS Journal, EarlyView.
Protein structure is defined by its surrounding water. Naturally, this is also true for the gigantic protein filaments that pathogenic bacteria use to attach to and colonize their host cells. Structures of these filaments for which water can be identified with high confidence have stayed elusive for the longest time.
Hiroya Oki, Hideki Shigematsu, Kazuki Kawahara, Christoph Gerle   +3 more
wiley   +1 more source

Unraveling the active site cover of coproheme decarboxylase from Listeria monocytogenes

The FEBS Journal, EarlyView.
During heme biosynthesis in Gram‐positive bacteria, coproheme decarboxylase (ChdC) catalyzes the conversion of four‐propionate substrate coproheme to the two propionate product heme b. Its active site is universally covered by a flexible linking loop. This study identifies an important histidine residue, which stabilizes the loop in a ChdC homolog.
Nikolaus Falb, Dominik Hörtnagel, Chris Oostenbrink, Paul G. Furtmüller, Stefan Hofbauer   +4 more
wiley   +1 more source