Results 131 to 140 of about 3,653 (216)

Identification of a key water molecule involved in the macrophage migration inhibitory factor‐catalyzed tautomerization of para‐hydroxyphenylpyruvate using neutron crystallography

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Neutron crystallography was used to determine a 2.5‐Å resolution all‐atom structure of macrophage migration inhibitory factor (MIF) interacting with 3‐(4‐hydroxyphenyl)‐pyruvate (HPP). MIF is a pro‐inflammatory, pro‐tumorigenic protein that may be an attractive therapeutic target. MIF catalyzes the interconversion of the keto and enol forms of
Gabriela C. Schröder, Gregg V. Crichlow, Ewelina Jablonowski, Georgios Pantouris, Jay Nix, Naomi Chayen, Flora Meilleur, Elias J. Lolis   +7 more
wiley   +1 more source

Quaternary structures of Streptococcus pneumoniae nucleoside diphosphate kinase: From hexamers to supramolecular assemblies

Protein Science, Volume 35, Issue 9, September 2026.
Abstract The nucleoside diphosphate kinase (NDK) is a key enzyme that controls the balance of nucleotide pools in all living organisms. Beyond this fundamental role, NDKs exert pleiotropic effects in many cellular processes, including cell development, signal transduction, differentiation, tumor metastasis, and gene expression. The quaternary structure
Julie Kerboeuf, Paul Nouri, Frédéric Galisson, Laetitia Daury, Marie‐France Giraud, Olivier Lambert, Cédric Orelle, Lionel Ballut, Elise Kaplan, Jean‐Michel Jault, Cécile Gonzalez   +10 more
wiley   +1 more source

Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography

, 2023
Gotthard G, Mous S, Weinert T, Antonelli Maia RN, James D, Dworkowski F, Gashi D, Furrer A, Ozerov D, Panepucci E, Wang M, Schertler GFX, Heberle J, Standfuss J, Nogly P.   +14 more
europepmc   +1 more source

Total synthesis and structural characterization of a novel protein scaffold from the snail Biomphalaria glabrata

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Disulfide‐rich miniproteins constitute compact and highly stable scaffolds of growing interest for molecular and structural engineering. Schistosomins are ~80‐residue proteins conserved across gastropods that form a long‐standing orphan family whose structure and biological roles have remained unknown.
Oleg Melnyk, Stéphanie Caby, Armelle Vigouroux, Christine Demanche, Rémi Desmet, Magalie Sénéchal, Benoît Snella, Alexandra Mougel, Céline Boidin‐Wichlacz, Aurélie Parmentier, Ugo Pasco, Sonia Cantel, Solange Moréra, Jérôme Vicogne   +13 more
wiley   +1 more source

The C117D oxidation mimic reveals the monomeric structure of SARS‐CoV‐2 main protease

Protein Science, Volume 35, Issue 9, September 2026.
Abstract The SARS‐CoV‐2 main protease (Mpro) is essential for viral replication and functions as a homodimer, with dimerization being critical for catalytic activity. Mpro contains an unusually high number of cysteine residues. Among these, C117 and the catalytic nucleophile C145 can form a reversible disulfide bond under oxidative conditions.
Sam Andress, Matthew J. McLeod, Todd Holyoak   +2 more
wiley   +1 more source

A cryptic allosteric pocket shapes isoform‐selective inhibition of human malic enzymes

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Malic enzymes (ME) regulate central carbon metabolism and cellular redox balance, and the mitochondrial isoform ME2 is frequently upregulated in aggressive cancers to support metabolic flexibility and stress resistance. Isoform‐selective inhibition has remained out of reach because the catalytic machinery is essentially invariant across the ...
Ben A. Krinkel, Yuliana Yosaatmadja, Mark D. Slayton, Anita Krinkel, Jack Copping, Jin Heon Jeon, Joyce Eu, Kerslee Kohagen, Amir Ashoorzadeh, Jeff Smaill, Jack Flanagan, Christopher Walker, Sofia D. Merajver, Kerry Loomes, Christopher J. Squire   +14 more
wiley   +1 more source

Disulfide engineering of the FANCM–MHF complex reveals constraints on crosslink design in symmetric oligomers

Protein Science, Volume 35, Issue 9, September 2026.
Abstract The Fanconi anemia complementation group M protein (FANCM)–MHF complex is required for branched DNA recognition in the Fanconi anemia pathway, but structural analysis of the intact complex has been hindered by dissociation of FANCM from the FANCM‐associated histone fold (MHF) heterotetramer under crystallization conditions.
Sho Ito, Tatsuya Nishino
wiley   +1 more source

Introduction to the virtual thematic issue on room-temperature biological crystallography

IUCrJ, 2023
Roberto A. Steiner
doaj   +1 more source

Data of fixed-target pink-beam serial synchrotron crystallography at the Pohang Light Source II. [PDF]

Data Brief
Kim Y, Nam KH.
europepmc   +1 more source

Structural and Biophysical Characterization of C‐Glycosylic 1,2‐Thiodisaccharides Reveals Determinants of Selective Binding to Galectin‐7 and Galectin‐8N

ChemMedChem, Volume 21, Issue 16, 27 August 2026.
The molecular basis of galectin recognition by C‐glycosylic 1,2‐thiodisaccharides is revealed. While Gly‐1 provides a conserved affinity driving interaction, Gly‐2 governs isoform selectivity through contacts with variable CRD regions. Unique galectin‐8N residues are identified as attractive handles for the development of next‐generation selective ...
Anastasia S. Tsagkarakou, Anastassia L. Kantsadi, Vasiliki I. Theodoridou, Nikolaos Veliotis, László Lázár, János József, László Juhász, George Kontopidis, Hakon Leffler, Ulf J. Nilsson, László Somsák, Demetres D. Leonidas   +11 more
wiley   +1 more source