Results 131 to 140 of about 7,535 (227)

Microfluidic rotating-target device capable of three-degrees-of-freedom motion for efficient in situ serial synchrotron crystallography. [PDF]

J Synchrotron Radiat, 2023
Zhao FZ, Wang ZJ, Xiao QJ, Yu L, Sun B, Hou Q, Chen LL, Liang H, Wu H, Guo WH, He JH, Wang QS, Yin DC.   +12 more
europepmc   +1 more source

Structural and functional characterization of the newly identified Photorhabdus laumondii tumor necrosis factor‐like lectin

The FEBS Journal, EarlyView.
We characterize the PLTL lectin from Photorhabdus laumondii, which adopts a unique TNF‐like fold. Binding assays reveal a preference for branched histo‐blood group oligosaccharides. Protein crystallography uncovers a novel hexameric assembly and identifies key residues involved in carbohydrate recognition.
Filip Melicher, Pavel Dobeš, Jan Komárek, Lukáš Faltinek, Marek Korsák, Petra Sýkorová, Josef Houser, Michaela Wimmerová   +7 more
wiley   +1 more source

Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2021
Illava G, Jayne R, Finke AD, Closs D, Zeng W, Milano SK, Huang Q, Kriksunov I, Sidorenko P, Wise FW, Zipfel WR, Apker BA, Thorne RE.   +12 more
europepmc   +1 more source

Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action. [PDF]

, 2018
Bioactive natural products have evolved to inhibit specific cellular targets and have served as lead molecules for health and agricultural applications for the past century1-3.
Bat-Erdene, Undramaa, Gan, Jianhua, Jacobsen, Steven E, Liu, Qikun, Nguyen, Calvin, Tang, Yi, Yan, Yan, Yuan, Shuguang, Zang, Xin, Zhou, Jiahai   +9 more
core  

Anemia‐associated mutations disrupt the CDIN1‐Codanin1 complex in inherited congenital dyserythropoietic anemia I (CDA‐I) disease

The FEBS Journal, EarlyView.
Congenital dyserythropoietic anemia type I (CDA‐I) arises from mutations in Codanin1 and CDIN1. Using quantitative biophysical approaches, we show that disease‐associated mutations disrupt the CDIN1‐Codanin1 complex. Our findings provide critical insights into the molecular mechanism that links protein dysfunction to disturbing chromatin arrangement ...
Martin Stojaspal, Tomas Brom, Ivona Nečasová, Tomáš Janovič, Pavel Veverka, Naina Verma, Lukáš Uhrík, Lenka Hernychova, Ctirad Hofr   +8 more
wiley   +1 more source

Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography data

Structural Dynamics
With the development of serial crystallography at both x-ray free electron laser and synchrotron radiation sources, time-resolved x-ray crystallography is increasingly being applied to study conformational changes in macromolecules.
Adams Vallejos, Gergely Katona, Richard Neutze   +2 more
doaj   +1 more source

Soft x-ray absorption spectroscopy of metalloproteins and high-valent metal-complexes at room temperature using free-electron lasers [PDF]

, 2017
X-ray absorption spectroscopy at the L-edge of 3d transition metals provides unique information on the local metal charge and spin states by directly probing 3d-derived molecular orbitals through 2p-3d transitions.
Agapie, Theodor, Han, Zhiji, Kanady, Jacob S., Kubin, Markus, Suseno, Sandy, Tsui, Emily Y.   +5 more
core   +1 more source

The cytokinesis regulator RacGAP1 is a Rac1‐specific GAP on membranes

Protein Science, Volume 35, Issue 3, March 2026.
Abstract Rho family small GTPases are essential for cytokinesis completion. RacGAP1, a dimeric multidomain protein with a lipid‐binding C1 domain and a GTPase‐activating protein (GAP) domain, is a major regulator in this process. However, despite many cellular and biochemical studies, whether RhoA or Rac1 is the actual substrate inactivated by RacGAP1 ...
Pavlina Dubois, Yann Ferrandez, Clara Rey, Cyril Pages, Agata Nawrotek, Jacqueline Cherfils   +5 more
wiley   +1 more source

Prokaryotic PfaB is a terminal acyltransferase that determines the final PUFA product

Protein Science, Volume 35, Issue 3, March 2026.
Abstract Omega‐3 polyunsaturated fatty acids (PUFAs) are essential for human health due to their numerous beneficial biological properties. These compounds are synthesized in marine bacteria and eukaryotic microalgae by PUFA megasynthases (Pfas), which are evolutionarily related to fatty acid synthases (FAS) and polyketide synthases (PKS). In FAS, PKS,
Nahuel Lofeudo, Aurora Martín, Mateo Jácome, Xia Wan, María Lucas, Gabriel Moncalián   +5 more
wiley   +1 more source

Ultra-fast raster-scanning synchrotron serial micro-crystallography [PDF]

Acta Crystallographica Section A Foundations and Advances, 2019
Martin R. Fuchs, Wuxian Shi, Yuan Gao, Babak Andi, Jean Jakoncic, Edwin O. Lazo, Alexei Soares, Stuart F. Myers, John Skinner, Qun Liu, Herbert J. Bernstein, Evgeny Nazaretski, Sean McSweeney   +12 more
openaire   +1 more source