Results 201 to 210 of about 3,647 (218)

Serial Synchrotron X-Ray Crystallography (SSX)

2017
Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set ...
Diederichs K, Wang M
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Room-temperature serial synchrotron crystallography of apo PTP1B

2022
Abstract Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but a common hurdle is obtaining sufficiently large protein crystals. Serial synchrotron crystallography (SSX) helps address this hurdle by allowing the use of many medium- to small-sized crystals.
Shivani Sharma, Ali Ebrahim, Daniel A. Keedy   +2 more
openaire   +1 more source

Liquid application method for time-resolved analyses by serial synchrotron crystallography

Nature Methods, 2019
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times.
Pedram Mehrabi, Eike C. Schulz, Michael Agthe, Sam Horrell, Gleb Bourenkov, David von Stetten, Jan-Philipp Leimkohl, Hendrik Schikora, Thomas R. Schneider, Arwen R. Pearson, Friedjof Tellkamp, R. J. Dwayne Miller   +11 more
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The serial millisecond crystallography instrument at the Australian Synchrotron incorporating the “Lipidico” injector

Review of Scientific Instruments, 2019
A serial millisecond crystallography (SMX) facility has recently been implemented at the macromolecular crystallography beamline, MX2 at the Australian Synchrotron. The setup utilizes a combination of an EIGER X 16M detector system and an in-house developed high-viscosity injector, “Lipidico.” Lipidico uses a syringe needle to extrude the microcrystal ...
P. Berntsen, M. Hadian Jazi, M. Kusel, A. V. Martin, T. Ericsson, M. J. Call, R. Trenker, F. G. Roque, C. Darmanin, B. Abbey   +9 more
openaire   +2 more sources

Processing serial synchrotron crystallography diffraction data with DIALS

This chapter describes additions to the DIALS software package for processing serial still-shot crystallographic data, and the implementation of a pipeline, xia2.ssx, for processing and merging serial crystallography data using DIALS programs. To integrate partial still-shot diffraction data, a 3D gaussian profile model was developed that can describe ...
James, Beilsten-Edmands, James M, Parkhurst, Graeme, Winter, Gwyndaf, Evans   +3 more
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Developing mix-and-inject serial crystallography at synchrotrons

Acta Crystallographica Section A Foundations and Advances, 2023
K. A. Zielinski, L. Pollack
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Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality

2021
For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis
Mehrabi, Pedram, Bücker, Robert, Bourenkov, G., Ginn, H. M., von Stetten, D., Müller-Werkmeister, Henrike (Prof. Dr.), Kuo, A., Morizumi, T., Eger, B. T., Ou, W-L, Oghbaey, S., Sarracini, A., Besaw, J. E., Pare-Labrosse, O., Meier, S., Schikora, H., Tellkamp, F., Marx, A., Sherrell, D. A., Axford, D., Owen, R. L., Ernst, O. P., Pai, E. F., Schulz, Eicke C., Miller, R. J. D.   +24 more
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Development of serial protein crystallography with synchrotron radiation

Développement de la cristallographie sérielle de protéine utilisant le rayonnement synchrotron Le rayonnement synchrotron est l'un des facteurs clés du grand succès de la cristallographie macromoléculaire au cours des dernières décennies.
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Implementation and development of serial synchrotron crystallography at MAX IV

2023
Over the past decade, remarkable advancements in femtosecond X-ray free-electron lasers (XFELs) have brought about a profound transformation in structural biology. These XFELs have opened up exciting opportunities for conducting high-time resolution, room-temperature studies on protein structures and dynamics.
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Living cells: crystallization chambers for serial synchrotron crystallography

Acta Crystallographica Section A Foundations and Advances, 2022
J. Blaha, S. Panneerselvam, D. Schraivogel, B. Ramsz, D. Ordonez, M. Paulsen, T.R. Schneider, L.M. Steinmetz, M. Wilmanns   +8 more
openaire   +1 more source