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Liquid application method for time-resolved analyses by serial synchrotron crystallography
Nature Methods, 2019We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times.
Pedram Mehrabi +11 more
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Room-temperature serial synchrotron crystallography of apo PTP1B
2022AbstractRoom-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but a common hurdle is obtaining sufficiently large protein crystals. Serial synchrotron crystallography (SSX) helps address this hurdle by allowing the use of many medium- to small-sized crystals. We have used a recently introduced serial
Shivani Sharma +2 more
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Serial Synchrotron X-Ray Crystallography (SSX)
2017Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set ...
Kay, Diederichs, Meitian, Wang
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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
Journal of Visualized Experiments, 2020A facility for performing serial crystallography measurements has been developed at the Australian synchrotron. This facility incorporates a purpose built high viscous injector, Lipidico, as part of the macromolecular crystallography (MX2) beamline to measure large numbers of small crystals at room temperature.
Peter, Berntsen +4 more
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Processing serial synchrotron crystallography diffraction data with DIALS
This chapter describes additions to the DIALS software package for processing serial still-shot crystallographic data, and the implementation of a pipeline, xia2.ssx, for processing and merging serial crystallography data using DIALS programs. To integrate partial still-shot diffraction data, a 3D gaussian profile model was developed that can describe ...James, Beilsten-Edmands +3 more
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Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality
2021For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis
Mehrabi, Pedram +24 more
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Developing mix-and-inject serial crystallography at synchrotrons
Acta Crystallographica Section A Foundations and Advances, 2023K. A. Zielinski, L. Pollack
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Living cells: crystallization chambers for serial synchrotron crystallography
Acta Crystallographica Section A Foundations and Advances, 2022J. Blaha +8 more
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UV-related cataract formation: insights from serial synchrotron crystallography
Acta Crystallographica Section A Foundations and Advances, 2022Jake Hill, Yvonne Nyathi, Briony Yorke
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