Results 11 to 20 of about 5,999 (124)
Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]
Synthetic and Systems Biotechnology, 2020 Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Hong Gao +4 more
doaj +4 more sources
The site-specific integration reaction of
Mobile DNA, 2013 Background A large subfamily of serine recombinases contains long polypeptide segments appended to the C-terminal end of the conserved catalytic domain.
Mandali Sridhar +4 more
doaj +5 more sources
SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems. [PDF]
Nucleic Acids Res, 2022 AbstractSerine integrases are emerging as one of the most powerful biological tools for synthetic biology. They have been widely used across genome engineering and genetic circuit design. However, developing serine integrase-based tools for directly/precisely manipulating synthetic biobricks is still missing.
Ba F, Liu Y, Liu WQ, Tian X, Li J.
europepmc +4 more sources
Serine Integrase attP Binding and Specificity. [PDF]
J Mol Biol, 2018 Serine integrases catalyze the site-specific insertion of viral DNA into a host's genome. The minimal requirements and irreversible nature of this integration reaction have led to the use of serine integrases in applications ranging from bacterial memory storage devices to gene therapy.
Li H +4 more
europepmc +4 more sources
Coiled-coil interactions mediate serine integrase directionality. [PDF]
Nucleic Acids Res, 2017 Serine integrases are bacteriophage enzymes that carry out site-specific integration and excision of their viral genomes. The integration reaction is highly directional; recombination between the phage attachment site attP and the host attachment site attB to form the hybrid sites attL and attR is essentially irreversible.
Gupta K +4 more
europepmc +4 more sources
Phage-encoded Serine Integrases and Other Large Serine Recombinases [PDF]
Microbiology Spectrum, 2015 ABSTRACT The large serine recombinases (LSRs) are a family of enzymes, encoded in temperate phage genomes or on mobile elements, that precisely cut and recombine DNA in a highly controllable and predictable way. In phage integration, the LSRs act at specific sites, the attP site in the phage and the
Pallavi, Ghosh +2 more
+9 more sources
The ins and outs of serine integrase site-specific recombination. [PDF]
Curr Opin Struct Biol, 2014 Serine integrases catalyze the integration and excision of phage genomes into and out of bacterial chromosomes in a highly specific and directional manner, making these proteins powerful tools for genome engineering. In 2013, the first structure of a serine integrase-DNA complex was reported. This work revealed how the phage attP sequence is recognized
Rutherford K, Van Duyne GD.
europepmc +4 more sources
A single-input binary counting module based on serine integrase site-specific recombination. [PDF]
Nucleic Acids Res, 2019 A device that counts and records the number of events experienced by an individual cell could have many uses in experimental biology and biotechnology. Here, we report a DNA-based 'latch' that switches between two states upon each exposure to a repeated stimulus.
Zhao J +4 more
europepmc +6 more sources
Control of serine integrase recombination directionality by fusion with the directionality factor. [PDF]
Nucleic Acids Res, 2017 Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL and attR sites.
Olorunniji FJ +5 more
europepmc +7 more sources
Serine Integrases: Advancing Synthetic Biology [PDF]
ACS Synthetic Biology, 2018 Serine integrases catalyze precise rearrangement of DNA through site-specific recombination of small sequences of DNA called attachment (att) sites. Unlike other site-specific recombinases, the recombination reaction driven by serine integrases is highly directional and can only be reversed in the presence of an accessory protein called a recombination
Christine A. Merrick +2 more
openaire +3 more sources