Results 41 to 50 of about 5,999 (124)
SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells
Nature Communications, 2023 Chromatin boundary elements contribute to the partitioning of mammalian genomes into topological domains to regulate gene expression. Certain boundary elements are adopted as DNA insulators for safe and stable transgene expression in mammalian cells ...
Meng Zhang +5 more
doaj +1 more source
Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology
Microorganisms, 2019 Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research.
Grant A. R. Gale +5 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2021 Bacteriophages (phages) are viruses that infect bacteria. They are the most abundant biological entity on Earth (current estimates suggest there to be perhaps 1031 particles) and are found nearly everywhere.
Antonio J. Martín-Galiano +2 more
doaj +1 more source
Variable orthogonality of RDF – large serine integrase interactions within the ϕC31 family
AbstractLarge serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage DNA into the host genome.
MacDonald AI +6 more
europepmc +3 more sources
Site‐specificity of serine integrase demonstrated by the attB sequence preference of ɸ
FEBS Letters, 2018 Serine integrases mediate site‐specific recombination and are extensively applied in genetic engineering and synthetic biology. However, which regions of the attachment sites determine site‐specificity and how these regions function in recombination remain elusive.
Xiaolai, Lei +3 more
openaire +2 more sources
Critical involvement of the ATM-dependent DNA damage response in the apoptotic demise of HIV-1-elicited syncytia. [PDF]
PLoS ONE, 2008 DNA damage can activate the oncosuppressor protein ataxia telangiectasia mutated (ATM), which phosphorylates the histone H2AX within characteristic DNA damage foci.
Jean-Luc Perfettini +20 more
doaj +1 more source
Applications of Serine Integrases in Synthetic Biology over the Past Decade
SynBio, 2023 Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology. In this review, we aim to systematically summarize the various studies ranging from structure and the catalytic mechanism to genetic design ...
Fang Ba +4 more
openaire +1 more source
Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay
Advanced Science, EarlyView.This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao +6 more
wiley +1 more source
Advanced Science, EarlyView.Emerging evidence suggests that intraneuronal Aβ accumulation represents an early pathogenic event in Alzheimer's disease (AD). Using Drosophila AD model, this study shows that a nonsecreted serine protease Yip7 physically interacts with Aβ. This causes intraneuronal Aβ accumulation but surprisingly reduces the associated neurotoxicity, arguing that ...
Jingyun Su +4 more
wiley +1 more source
Frontiers in Microbiology, 2020 It is well known that bacteriophages play crucial roles in many aspects, such as controlling the number and the diversity of bacteria and participating in horizontal gene transfer, which is a key process in the evolution of bacteria. However, so far, the
Xinyan Yu +5 more
doaj +1 more source