Results 51 to 60 of about 5,999 (124)

Multi‐Targeting Non‐Specific Genome Engineering in Bacteria

Advanced Science, EarlyView.
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun, Ruixiang You, Yiwen Zhou, Huiyan He, Wenfang Wang, Xudong Qu, Yinhua Lu, Lei Li   +7 more
wiley   +1 more source

Ultrasound‐Responsive Engineered Bacteria for Targeted Cancer Therapy: Strategies, Mechanisms, and Applications

Advanced Science, EarlyView.
This review elucidates URB design strategies, including genetic and physicochemical modifications, while dissecting the synergistic mechanisms governing spatiotemporal control. It further evaluates applications in targeted drug delivery, gene modulation, and immunotherapy, finally offering perspectives on overcoming translational barriers for clinical ...
Xueyao Wang, Wenjing Xu, Yafang Lu, Huaibin Yu, Zhengbao Zha, Xijun Zhang   +5 more
wiley   +1 more source

Adaptation of the invasive pest Drosophila suzukii to a specialized nutritional niche

Insect Science, EarlyView.
Unlike most Drosophila larvae that feed on spoiled food, Drosophila suzukii larvae thrive on ripening fruits and consequently face a low‐protein, high‐carbohydrate nutritional challenge. Comparisons of growth among D. suzukii, D. biarmipes, and D. melanogaster larvae across diets with varying protein‐to‐carbohydrate ratios demonstrate that D.
Yan Hou, Ying Zhen
wiley   +1 more source

Therapeutic Gene Editing: DNA Repair Pathways, Emerging Editors, and Clinical Progress

iNew Medicine, Volume 2, Issue 1, March 2026.
ABSTRACT The field of gene editing has evolved rapidly over the past decade, progressing from programmable zinc‐finger nucleases (ZFNs) and transcription activator‐like effector nucleases (TALENs) to the widespread adoption of CRISPR‐Cas systems. First‐generation editors catalyzed genome engineering by introducing targeted double‐strand breaks (DSBs ...
Li‐Kuang Tsai, Youcai Xiong, Dongqi Liu, Dongshan Yang, Jifeng Zhang, Jie Xu, Renzhi Han, Y. Eugene Chen   +7 more
wiley   +1 more source

Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages

mBio, 2020
Temperate bacteriophages are common and establish lysogens of their bacterial hosts in which the prophage is stably inherited. It is typical for such prophages to be integrated into the bacterial chromosome, but extrachromosomally replicating prophages ...
Katherine S. Wetzel, Haley G. Aull, Kira M. Zack, Rebecca A. Garlena, Graham F. Hatfull   +4 more
doaj   +1 more source

Mycochemical Diversity and Therapeutic Potential of Hymenochaetoid Fungi from Central Asia: Regional and Global Perspectives

New Zealand Journal of Botany, Volume 64, Issue 1, March 2026.
Central Asia harbors a rich yet understudied assemblage of wood‐inhabiting Hymenochaetoid fungi. This review delivers the first comprehensive synthesis of 43 poroid species representing 18 genera documented across montane forests, steppes, and xeric habitats.
Yusufjon Gafforov, Milena Rašeta, Sylvie Rapior, Manzura Yarasheva, Oksana Mykchaylova, Rustamjon Allaberdiev, Şule İnci, Dilfuza Berdieva, Arshad Mehmood Abbasi, Shikhnazar Khodjaev, Dian‐Ming Hu, Weijun Yang, Abudujilili Abuduaini, Maria Letizia Taddei, Mengcen Wang, Islomjon Urinboev, Jiajia Chen   +16 more
wiley   +1 more source

The mutation of Transportin 3 gene that causes limb girdle muscular dystrophy 1F induces protection against HIV-1 infection.

PLoS Pathogens, 2019
The causative mutation responsible for limb girdle muscular dystrophy 1F (LGMD1F) is one heterozygous single nucleotide deletion in the stop codon of the nuclear import factor Transportin 3 gene (TNPO3).
Sara Rodríguez-Mora, Flore De Wit, Javier García-Perez, Mercedes Bermejo, María Rosa López-Huertas, Elena Mateos, Pilar Martí, Susana Rocha, Lorena Vigón, Frauke Christ, Zeger Debyser, Juan Jesús Vílchez, Mayte Coiras, José Alcamí   +13 more
doaj   +1 more source

Diversity of FAAL enzymes and prediction of their substrate specificity using FAALPred

Protein Science, Volume 35, Issue 2, February 2026.
Abstract FAALs (fatty acyl‐AMP ligases) recruit and incorporate fatty acids during the biosynthesis of secondary metabolites. Their diversity, distribution, and substrate specificity remain poorly understood, which limits functional predictions from sequence data.
Anne Liong, Leandro de Mattos Pereira, Pedro N. Leão   +2 more
wiley   +1 more source

Diversity, Function and Activity of DNA Viruses in the Qiangyong Proglacial Lake Sediment, the Tibetan Plateau

Environmental Microbiology Reports, Volume 18, Issue 1, February 2026.
This study identified DNA viruses from Qiangyong proglacial lake sediments on the Tibetan Plateau, revealing a highly diverse and previously uncharacterized viral community. These viruses influence microbial community structure and function by infecting key nutrient‐transforming taxa and encoding auxiliary metabolic genes that modulate host metabolism.
Yang Zhao, Meiling Feng, Hongfei Chi, Keshao Liu, Rong Wen, Weizhen Zhang, Pengfei Liu   +6 more
wiley   +1 more source

Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP Sites

G3: Genes, Genomes, Genetics, 2018
Precise modification of sequences in the Drosophila melanogaster genome underlies the powerful capacity to study molecular structure-function relationships in this model species.
Beatriz Blanco-Redondo, Tobias Langenhan
doaj   +1 more source