Results 231 to 240 of about 730,822 (283)

Serum-free media for periosteal chondrogenesis in vitro

Journal of Orthopaedic Research, 2004
AbstractOrgan culture studies involving whole explants of periosteum have been useful for studying chondrogenesis, but to date the standard culture model for these explants has required the addition of fetal bovine serum to the media. Numerous investigators have succeeded in culturing chondrocytes and embryonic cells in serum‐free conditions but there ...
James S Fitzsimmons   +2 more
exaly   +3 more sources

Development of Serum-Free Media for Lepidopteran Insect Cell Lines

open access: yes, 2016
Baculovirus-based Insect Cell Technology (ICT) is widely used for the expression of recombinant heterologous proteins and baculovirus bioinsecticides, and has recently gained momentum as a commercial manufacturing platform for human and veterinary vaccines.
Chan, Leslie C. L., Reid, Steven
openaire   +6 more sources

Serum-free media for lymphoid culture

Journal of Biotechnology, 1990
A serum-free medium is described that we have been using since almost ten years when growing a number of lymphoid cell lines. Serum-free media offer several advantages before media containing even well standardized animal sera. As an example of studies where serum-free media offer advantages we present data demonstrating the use of a defined medium ...
exaly   +3 more sources

Serum-free media for cultures of primitive and mature hematopoietic cells

Biotechnology and Bioengineering, 1994
AbstractThe in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum‐containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum‐free media.
William M Miller   +1 more
exaly   +3 more sources

IgG production kinetics in serum free media

Biotechnology Letters, 1986
A murine hybridoma (455) was cultured in four different serum free media formulations, and a newborn calf serum supplemented medium was used as a basis of comparison. The serum supplemented medium supports a higher cell growth rate and results in a higher IgG titer.
Pao C Chau
exaly   +2 more sources

Development of Serum-Free Media for Lepidopteran Insect Cell Lines

open access: yes, 2007
Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part of one of the most successful eukaryotic expression systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring
Spiros N. Agathos, Agathos, Spiros N.
openaire   +3 more sources

Serum-Free Media

1991
Serum, often perceived as necessary for cell growth in vitro, has been used in establishing most conventional primary cell culture systems and continuous cell lines. Serum provides hormones and nutrients which promote cell growth. However cells in vivo are not normally exposed to serum, a result of the clotting of plasma.
Paul Collodi   +2 more
openaire   +3 more sources

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