Results 241 to 250 of about 730,822 (283)
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2020
The rapidly increasing trend to employ baculovirus expression vector system for the expression of foreign genes in insect cells has established a need for serum-free media (SFM) development for use in large-scale expression systems. All cultured insect cell lines up to have been initiated and established in media fortified with fetal bovine sera and ...
Stefan A. Weiss +3 more
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The rapidly increasing trend to employ baculovirus expression vector system for the expression of foreign genes in insect cells has established a need for serum-free media (SFM) development for use in large-scale expression systems. All cultured insect cell lines up to have been initiated and established in media fortified with fetal bovine sera and ...
Stefan A. Weiss +3 more
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Selection of transfored cells in serum-free media
In Vitro Cellular & Developmental Biology, 1985NIH3T3 cells grow in a serum-free basal nutrient medium supplemented with fibronectin, transferrin, insulin, epidermal growth factor (EGF) and high density lipoprotein (HDL). The individual omission from the serum-free medium of insulin, EGF, or HDL results in greatly reduced cell growth.
L C, Chiang +3 more
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Production of Monoclonal Antibodies in Serum-free Media
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2015Four hybridoma cell lines (C4B, 10C2G5, 6C5F4C7, 2D10G11) were adapted to grow in serum-free conditions. Cell proliferation, viability, and antibody production in Nutridoma SP and Ex-Cell HSF 610 media were evaluated and results compared with those obtained using DMEM containing 10% fetal bovine serum (control medium).
Laura, Manna +6 more
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Production of recombinant proteins in serum-free media
Cytotechnology, 1991The advantages of serum-free culture for the manufacture of recombinant biopharmaceuticals from mammalian cells are reviewed. The process favoured is fed-batch serum-free cell culture. This process is applicable to the majority of cell lines, is practical on the large scale, gives the lowest manufacturing cost, and can be carried out without the use of
D, Broad, R, Boraston, M, Rhodes
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Serum-free media for cultivation of spiroplasmas
Canadian Journal of Microbiology, 1989Serum-free media that contained bovine serum albumin and lipids (fatty acids, cholesterol, phosphatidic acid, and phospholipids) to replace serum were formulated for the culture of some fastidious strains of corn stunt spiroplasma, Spiroplasma kunkelii. These media also supported good growth of other spiroplasmas, including epiphytic, entomopathogenic,
I.-M. Lee, R. E. Davis
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1998
The development and routine use of serum-free media (SFM) is a high priority for cell culture from both an industrial and scientific point of view. With the extent of specific nutritional and supplemental requirements exhibited by different cell types and the possible combinations that can be used, the task of finding a selection of factors that can ...
Joanne Keenan +2 more
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The development and routine use of serum-free media (SFM) is a high priority for cell culture from both an industrial and scientific point of view. With the extent of specific nutritional and supplemental requirements exhibited by different cell types and the possible combinations that can be used, the task of finding a selection of factors that can ...
Joanne Keenan +2 more
openaire +1 more source
Strategies for optimising serum-free media
Cytotechnology, 1991Two of the main reasons for developing serum-free medium are to improve the growth and to increase the productivity of a cell. This review has attetmpted to show that these goals can be achieved far more easily than generally realised by utilising the methods that have been developed during the past decade and by using a limited number of standard ...
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Adaptation of Mammalian Cells to Growth in Serum-Free Media
Molecular Biotechnology, 2000A three-step protocol is described for adapting an anchorage-dependent, serum-dependent recombinant mammalian cell lineage to high density serum-free suspension culture. The objective is a cell lineage that is well-suited for the manufacture of a recombinant protein.
M S, Sinacore, D, Drapeau, S R, Adamson
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Culture of cardiac muscle cells in serum-free media
Experimental Cell Research, 1981Abstract Cardiac muscle cells from neonatal rats have been cultured in completely defined serum-free media. The most successful system consists of precoating culture flasks with fibronectin at a concentration of 5 μg/cm2 of surface area and adding fetuin and either dibutyryl cyclic AMP (db-cAMP), cholera toxin, epidermal growth factor or insulin plus
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Serum‐free media in hybridoma culture and monoclonal antibody production
Biotechnology and Bioengineering, 1988AbstractThe replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum‐free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum‐free formulations and selection rules for the serum‐free ingredients. In general,
M C, Glassy, J P, Tharakan, P C, Chau
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