Results 251 to 260 of about 730,822 (283)
Some of the next articles are maybe not open access.
Development of serum-free media
2000Abstract The removal of serum or other undefined components from cell culture medium involves a multifaceted approach. This is mainly due to the interactive nature of cell culture systems. Cell culture systems require various components: basal nutrients, a set of supplements that convey information, such as growth factors, mitogens ...
openaire +1 more source
The use of hormone-supplemented serum-free media in primary cultures
Experimental Cell Research, 1979Abstract Recent advances in tissue culture and endocrinology have made possible the growth of established cell lines in hormone-supplemented serum-free media. The hormone requirements differ for different cell types but are similar or identical for the same cell types.
Mather, J P, Sato, G H
openaire +2 more sources
Development of preimplantation guinea-pig embryos in serum-free media
Reproduction, Fertility and Development, 1993This study was undertaken to determine how far preimplantation guinea-pig embryos can develop normally in vitro with serum-free media generally used for mouse (Whitten's medium, WM) and rabbit (Kane's medium, KM) embryos. Preimplantation guinea-pig embryos were recovered from naturally mated females at each day of pregnancy between Day 1 and Day 6, and
O, Suzuki +5 more
openaire +2 more sources
Steroidogenesis in Porcine Follicle Cells Cultured in Serum-Free Media
Gynecologic and Obstetric Investigation, 2010The purpose of this study was to determine the steroid production of cultured porcine follicle cells grown in serum-free media. Theca cells (TC) and granulosa cells (GC) from large porcine follicles (greater than 8 mm) were dispersed and plated as monolayer cultures in serum-free media.
A L, Stein, G S, diZerega, S A, Tonetta
openaire +2 more sources
Optimizing Vendor Proprietary Serum-Free Media
1995In an environment of drug cost containment, it may be an efficient use of limited RD (ii) using statistically designed studies; and (iii) using media blends. The example used to illustrate this strategy involves growth and production of a recombinant CHO line in serum and protein-free medium developed by JRH Biosciences.
A. H. Newell +2 more
openaire +1 more source
Two novel serum-free media for the culture of Trichomonas vaginalis
Parasitology Research, 2006Definitive diagnosis of Trichomonas vaginalis, one of the common causes of sexually transmitted diseases in Turkey, relies on the microscopic examination of both fresh preparations and culture material of genital secretions and urine. The aim of this study was to compare the diagnostic efficacies of two culture media, serum-free TB1 and TB2, including ...
M E, Limoncu +5 more
openaire +2 more sources
Cholesterol: An important additive for serum-free media?
1994Synopsis In this study the influence of cholesterol in different form on serum-free cultured hybridoma cells was examined. The cells were able to grow without cholesterol. The addition of free cholesterol leads to an inhibited cell growth and metabolism activity.
H. Büntemeyer, M. Burg, J. Lehmann
openaire +1 more source
Differentiation of Myoblasts in Serum-Free Media: Effects of Modified Media Are Cell Line-Specific
Cells Tissues Organs, 2000Myoblast cell lines are grown and differentiated readily in cell culture. Two cell lines typically used for investigating the growth and differentiation of muscle are the mouse cell line C2C12 and the rat cell line L6. The differentiation of these cells in vitro requires a switch from a serum-rich medium to a less rich medium after the cells have ...
Lawson, M. A., Purslow, P. P.
openaire +2 more sources
Serum-free media for neuronal cell culture
1992Abstract The first reported work on the growth of cells in vitro was in 1907 by Harrison (1) who was able to suspend an explant of the spinal cord of a frog embryo from a microscope slide. This ‘hanging drop technique’ enabled the growth of the neuronal cells to take place within a fibrin matrix resulting from the application of a drop ...
openaire +1 more source