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[Cultivation of mammalian cells in serum-free media].
Human cell, 1992P3 cell lines can be grown in protein- and lipid-free synthetic medium. Using the P3 cell culture, we have shown that these cells produce autocrine growth factors and cell-substrate attachment factors. Because the cultured cells produce proteinase-inhibitor, spent medium is applicable for inactivating the action of trypsin at the time of cell passage ...
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The development, benefits and disadvantages of serum-free media.
Developments in biological standardization, 1999Serum-free culture is used routinely for many cell types, especially if transformed, e.g. CHO, hybridoma and recombinant myeloma cell lines. Serum-free medium reduces operating costs and process variability, and removes a potential source of infectious agents.
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Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production
International Journal of Pharmaceutics, 2012Maria Elisa Rodrigues +2 more
exaly
Physiological enhancement of immunoglobulin production of hybridomas in serum-free media
Cytotechnology, 1991H, Murakami +4 more
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