RPA primers and gRNAs for SFTSV DETECTR.
, 2022 RPA primers and gRNAs for SFTSV DETECTR.
Misun Kim (3089739) +5 more
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SFTSV NSs is involved in the retention of SAFA.
, 2021 (A) MEF cells were transfected with Flag-tagged SFTSV NSs for 24 h. The nuclear and cytoplasmic protein was separated. SAFA, Lamin A, and Rab5 protein levels were analyzed by Western blot.
Xue-jie Yu (523802) +2 more
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Application of SFTSV DETECTR to clinical samples.
, 2022 (A) RNA was extracted from patient plasma samples and subjected to RT-PCR for SFTSV detection. PCR products (124 bp) were visualized using gel electrophoresis. (B, C) HUDSON-treated patient plasma samples were used to amplify viral nucleic acids using RT-
Misun Kim (3089739) +5 more
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Ultra-rapid detection of nuclear protein of severe fever with thrombocytopenia syndrome virus by colloidal gold immunochromatography assay [PDF]
PeerJIn 2009, severe fever with thrombocytopenia syndrome virus (SFTSV), also known as the Dabie bandavirus (DBV), was first discovered in Henan, China. It is a tick-borne zoonotic virus with a fatality rate ranging from 6% to 30%. Currently, we lack safe and
Zhiwei Huang +13 more
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Two Different Strains of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) in North and South Osaka by Phylogenetic Analysis of Evolutionary Lineage: Evidence for Independent SFTSV Transmission [PDF]
Viruses, 2021 Severe fever with thrombocytopenia syndrome (SFTS) is a novel tick-borne infectious disease, therefore, the information on the whole genome of the SFTS virus (SFTSV) is still limited. This study demonstrates a nearly whole genome of the SFTSV identified in Osaka in 2017 and 2018 by next-generation sequencing (NGS).
Ryo Ikemori +8 more
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Optimization of the multiplex SFTSV/OT/IC LAMP primer set.
, 2022 (A) Different concentration ratios of SFTSV, OT, and IC primer sets (1.5:2:0.6, 1.5:1.5:0.6, and 2:1.5:0.6, respectively) for SFTSV, OT, and IC plasmid mixtures (1:1:1). (B) Temperature gradient tests (59–65°C) of the multiplex SFTSV/OT/IC LAMP assay.
Chae Seung Lim (1888042) +9 more
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Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China. [PDF]
PLoS ONE, 2014 Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the ...
Lei Zhang +9 more
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Knockout of CLTC gene reduces but not completely block SFTSV infection
PLOS ONE, 2023 Clathrin is a key protein for viruses to enter host cells. Previous studies often use clathrin inhibitors or gene knockdown technology to partially inhibit the function of clathrin, but whether SFTSV can infect host cells without clathrin expression remains unclear.
Tiezhu Liu +11 more
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The increased SAFA expression and cell viability under SFTSV infection.
, 2021 (A) MEF cells were infected with SFTSV (MOI = 10) for 12, 24, or 48 h. Cell viability was analyzed using Cell Counting Kit-8 (CCK8). (B) MEF cells were infected with SFTSV (MOI = 0, 1, 5, 10) for 48 h. Cell viability was analyzed using CCK8.
Xue-jie Yu (523802) +2 more
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Knockout of CLTC caused a decrease infection of SFTSV.
, 2023 A Comparison of SFTSV nucleoprotein in supernatant for CLTC-KO and wild-type cells. Culture supernatants were collected at 0h, 24h, 36h and 48h after infection, and subjected for ELISA detection for SFTSV nucleoprotein.
Jiajia Li (184267) +11 more
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