Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks [PDF]
BMC Microbiology, 2007 Background Shiga toxins 1 and 2 (Stx1 and Stx2) are bacteriophage-encoded proteins that have been associated with hemorrhagic colitis, hemolytic uremic syndrome and other severe disease conditions.
Farrell Larry D +5 more
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Intimin, Tir, and Shiga Toxin 1 Do Not Influence Enteropathogenic Responses to Shiga Toxin-Producing Escherichia coli in Bovine Ligated Intestinal Loops [PDF]
Infection and Immunity, 2002 ABSTRACT Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors ...
Stevens, Mark P. +7 more
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Molecular Damage and Induction of Proinflammatory Cytokines in Human Endothelial Cells Exposed to Shiga Toxin 1, Shiga Toxin 2, and α-Sarcin [PDF]
Infection and Immunity, 2007 ABSTRACT Treatment of human endothelial cells with Shiga toxin 1 and 2 leads to the upregulation of genes encoding proinflammatory molecules involved in the pathogenesis of hemolytic-uremic syndrome. The paradoxical effect of inhibitors of mRNA translation, such as Shiga toxins, that at the same time induce protein expression was investigated
BRIGOTTI, MAURIZIO +7 more
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The Effect of Mitomycin C on Induction of Shiga Toxin Production in Clinical STEC Isolates
ToxinsEarly determination of the Shiga toxin type of Shiga toxin-producing Escherichia coli (STEC) is crucial for guiding STEC-infected patients for proper and timely treatment and patient care.
Surangi H. Thilakarathna +2 more
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, 2014 Hemolytic uremic syndrome (HUS) is a potential life-threatening condition caused by infection with Shiga toxin-producing Escherichia coli O157:H7. There are two major types of Shiga toxins, namely, Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2), of which, Stx2 is clinically most closely associated with enterohemorrhagic E. coli O157:H7-mediated HUS. The
Jacobson, Jared M
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Protection against Shiga Toxin 1 Challenge by Immunization of Mice with Purified Mutant Shiga Toxin 1 [PDF]
Infection and Immunity, 2003 ABSTRACT Shiga toxin 1 (Stx1) of enterohemorrhagic Escherichia coli O157:H7 was cloned, and four mutant Stx1s were constructed by site-directed mutagenesis with PCR. The wild-type and mutant Stx1s with amino acid replacements at positions 167 and 170 of the A subunit were purified by one-step affinity ...
Satoshi, Ishikawa +7 more
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Shiga Toxin 2–Specific but Not Shiga Toxin 1–Specific Human Monoclonal Antibody Protects Piglets Challenged with EnterohemorrhagicEscherichia coliProducing Shiga Toxin 1 and Shiga Toxin 2 [PDF]
The Journal of Infectious Diseases, 2010 Escherichia coli strains that produce Shiga toxin 2 (Stx2) are isolated from hemolytic-uremic syndrome (HUS) cases more frequently than are strains that produce both Shiga toxin 1 (Stx1) and Stx2, whereas strains that produce only Stx1 are rarely isolated from HUS cases.
Kwang-Il, Jeong +2 more
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Characterization of Shigella dysenteriae 1 (Shiga) toxin purified by anti-Shiga toxin affinity chromatography [PDF]
Infection and Immunity, 1980 Shigella dysenteriae 1 (Shiga) toxin was purified from whole-cell lysates by antitoxin affinity column chromatography, radioiodination, and Sephacryl S-200 gel filtration of 125I-labeled affinity column eluates. Two chromatographic peaks were observed. The percentage of radioactivity in peak I samples immunoprecipitated with antitoxin ranged from 95 to
A D, O'Brien +3 more
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Immunochemical and cytotoxic activities of Shigella dysenteriae 1 (shiga) and shiga-like toxins [PDF]
Infection and Immunity, 1982 Toxins in culture supernatants and bacterial lysates of S. dysenteriae 1 and S. flexneri were quantitated by a cytotoxicity assay and a newly developed radioimmunoassay. Cytotoxin titers paralleled toxin antigen levels. Thus, variations in cytotoxicity among shigellae probably reflect differences in toxin yield rather than specific activity ...
A D, O'Brien, G D, Laveck
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In Vivo Transduction with Shiga Toxin 1-Encoding Phage [PDF]
Infection and Immunity, 1998 ABSTRACT To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn 10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken.
D W, Acheson +5 more
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