Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks [PDF]
BMC Microbiology, 2007 Background Shiga toxins 1 and 2 (Stx1 and Stx2) are bacteriophage-encoded proteins that have been associated with hemorrhagic colitis, hemolytic uremic syndrome and other severe disease conditions.
Farrell Larry D +5 more
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Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2 [PDF]
The International Journal of Biochemistry & Cell Biology, 2011 Shiga toxins produced by E. coli O157:H7 are responsible for food poisoning and hemolytic uremic syndrome (HUS). The A subunits of Shiga toxins (Stx1A and Stx2A) inhibit translation by depurinating a specific adenine in the large rRNA.
Jia-Chi Chiou +4 more
semanticscholar +3 more sources
Trafficking of Shiga toxin/Shiga-like toxin-1 in human glomerular microvascular endothelial cells and human mesangial cells. [PDF]
Kidney International, 2006 This study has determined the intracellular transport route of Shiga-like toxin (Stx) and the highly related Shiga toxin in human glomerular microvascular endothelial cells (GMVECs) and mesangial cells. In addition, the effect of tumor necrosis factor-alpha (TNF-alpha), which contributes to the pathogenesis of hemolytic-uremic syndrome, was evaluated ...
M. Warnier +7 more
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In Vivo Transduction with Shiga Toxin 1-Encoding Phage [PDF]
Infection and Immunity, 1998 ABSTRACT To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn 10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken.
D. Acheson +5 more
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Bovine macrophages sense Escherichia coli Shiga toxin 1 [PDF]
Innate Immunity, 2015 Shiga toxin (Stx)-producing Escherichia coli (STEC) infections in cattle are asymptomatic; however, Stx impairs the initiation of an adaptive immune response by targeting bovine peripheral and intraepithelial lymphocytes. As presumptive bovine mucosal macrophages (Mø) are also sensitive to Stx, STEC may even exert immune modulatory effects by acting ...
C. Menge +5 more
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Protection against Shiga Toxin 1 Challenge by Immunization of Mice with Purified Mutant Shiga Toxin 1 [PDF]
Infection and Immunity, 2003 ABSTRACT Shiga toxin 1 (Stx1) of enterohemorrhagic Escherichia coli O157:H7 was cloned, and four mutant Stx1s were constructed by site-directed mutagenesis with PCR. The wild-type and mutant Stx1s with amino acid replacements at positions 167 and 170 of the A subunit were purified by one-step affinity ...
S. Ishikawa +7 more
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The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1 [PDF]
Infection and Immunity, 2015 ABSTRACT Shiga toxin (Stx)-producing Escherichia coli (STEC) infections can lead to life-threatening complications, including hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS), which is the most common cause of acute renal failure in children in the United States.
D. Basu +5 more
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Shiga Toxin 2–Specific but Not Shiga Toxin 1–Specific Human Monoclonal Antibody Protects Piglets Challenged with EnterohemorrhagicEscherichia coliProducing Shiga Toxin 1 and Shiga Toxin 2 [PDF]
The Journal of Infectious Diseases, 2010 Escherichia coli strains that produce Shiga toxin 2 (Stx2) are isolated from hemolytic-uremic syndrome (HUS) cases more frequently than are strains that produce both Shiga toxin 1 (Stx1) and Stx2, whereas strains that produce only Stx1 are rarely isolated from HUS cases.
Kwang-Il, Jeong +2 more
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Characterization of Shigella dysenteriae 1 (Shiga) toxin purified by anti-Shiga toxin affinity chromatography [PDF]
Infection and Immunity, 1980 Shigella dysenteriae 1 (Shiga) toxin was purified from whole-cell lysates by antitoxin affinity column chromatography, radioiodination, and Sephacryl S-200 gel filtration of 125I-labeled affinity column eluates. Two chromatographic peaks were observed. The percentage of radioactivity in peak I samples immunoprecipitated with antitoxin ranged from 95 to
A D, O'Brien +3 more
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Immunochemical and cytotoxic activities of Shigella dysenteriae 1 (shiga) and shiga-like toxins [PDF]
Infection and Immunity, 1982 Toxins in culture supernatants and bacterial lysates of S. dysenteriae 1 and S. flexneri were quantitated by a cytotoxicity assay and a newly developed radioimmunoassay. Cytotoxin titers paralleled toxin antigen levels. Thus, variations in cytotoxicity among shigellae probably reflect differences in toxin yield rather than specific activity ...
A D, O'Brien, G D, Laveck
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